...few months: Lentiviral preps for expressing SARS-CoV-2 proteins ChR2 for targeted expression DREADDs GFP...2xStrep-IRES-Puro: Expression of the SARS-CoV-2 ORF3a Lentiviral preps for expressing the E protein (pLVX-EF1alpha-SARS-CoV...inventory of ready-to-use viral vectors. Here are some of the AAV and lentiviral preps we have released in ... and FLPo And more!  New lentiviral preps SARS-CoV-2 protein expression The Krogan lab quickly shared...plasmids into lentiviral preps to speed up COVID-19 research even further. The following lentiviral preps are...pAAV-Ef1a-fDIO mCherry Karl Deisseroth 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP Brandon...pAAV-hSyn-DIO-mCherry Bryan Roth 50457 AAV1 pAAV-hSyn-DIO-EGFP Bryan Roth 114472 AAVrg pAAV-hSyn-mCherry Karl...

...transgene expression across different cell types (Aschauer et al., 2013). Expression of hM4D(Gi)-mCherry from...stable Cas9-expressing cell line and then transduce this line with lentiviruses expressing various gRNAs...blast LentiCas9-blast is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute... is a dual-vector system, meaning it is typically used in conjunction with a second vector that delivers...lentiGuide-Puro. The alternative single-vector systems typically express Cas9 and a gRNA from the same plasmid...transductions with a single-vector system). Tips for using this plasmid Cas9 expression is typically cytotoxic...-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs from...

... with mCherry and you already have a transgenic mouse line that contains a Cre-dependent mCherry cassette...with mCherry controlled by a neuron specific promoter or you could breed your Cre-dependent mCherry line...your Cre-dependent mCherry mouse with the pmSyn-EBFP-Cre AAV. Because Cre expression is controlled by the...synapsin promoter, it and therefore mCherry will only be expressed in neurons. Indeed, this AAV provides... and other viral vector tools in the years to come. We’ll make many more viral vector tools and resources... A Versatile Tool for Gene Expression in Mammals Subscribe to Viral Vector Blog Posts Additional Resources... Service Page Browse Additional Viral Vectors Find Viral Vector Protocols ...

...in the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...protocols. Browse our AAV vector collection. Lentivirus Production Lentiviral preparations ("-LV” catalog... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Transducibility Some viral vectors are tested in vitro and in vivo for gene expression and/or function. These...Empty vector particles can be identified after negative staining and appear darker than full vector particles...

...pHAGE-TO-dCas9-3XmCherry . 72 h post transduction, cells were assayed for mCherry expression using fluorescence...marker. Determining the titer of your lentiviral vector allows you to control the multiplicity of infection...this protocol was developed using low titer lentiviral vectors (10 5 TU/mL). If you anticipate that your...Fluorescence Titering Assay for Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol ...rapidly thaw the lentiviral aliquot on ice prior to use. Prepare dilutions of the lentivirus into DMEM complete...Protocol that can be used to titer lentiviral preparations carrying a fluorescence marker. Protocols...Introduction This protocol can be used to titer lentiviral preparations that carry a fluorescence marker...

...available in a mammalian expression vector (pcDNA3) and a bacterial expression vector (pRSETB). Fabritius...like APOBEC. To achieve this they created a lentiviral vector system that monitors both APOBEC- and Cas9...reporter constitutively expresses eGFP to indicate reporter abundance, and mCherry fluorescence is activated...activity edits out a frame-shift mutation within the mCherry reporter. Quantification of editing efficiencies...efficiencies simply involves tracking the ratio of mCherry-positive to eGFP positive cells through fluorescence...both halves of the modified EB1 protein are overexpressed in cells, microtubule growth can be switched...

...create a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed with...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ...bacterial expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...collection of plasmids comprising the multiple lentiviral expression (MuLE) system from the laboratory of Ian... SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive (H1...-miR30 (CMV-TO) expression, or a promoterless construct. Pre-constructed Entry vectors containing Cas9...

... for each gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins...cells that are expressing your gene of interest at high levels. Unlike vectors that express screenable or...them. Why Use multicistronic vectors? Detecting cells that are expressing your gene, especially if you...want to express multiple genes in the same cell. Although it is possible to drive co-expression by using... with multiple, individual expression cassettes, having the genes expressed from the same cassette is ...relative expression levels between two or more genes is important. How do multicistronic vectors work? Scientists...transfer vectors with limited packaging capacity. Additionally, it may not be feasible to express more than...

...different vectors and flow cytometry is used to sort populations by mCherry and GFP expression to check...of robust expression of high G+C content genes, as validated through monitoring the expression of fluorescent...designed by putting together the coding sequence of a mCherry fluorescent protein followed by a stop codon and... be seen through the success of the change in expression of GFP, for the stop codon should have changed... 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker...

...p2A-ferritin-p2A-mCherry Mammalian Expression 74308 pcDNA3.0-Magneto2.0-p2A-mCherry Mammalian Expression 74334...protein expression constructs fused with epitopes or fluorophores, generate expression vectors with various...AAV transfer vector for in-vivo expression and plasmids optimized for dCas9-VPR expression in drosophila...parasites that constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides ... HEK293FT cells expressing a single gRNA targeting a single gene. The gRNA expressing cells were then ... to test multiple expression systems before they achieve the appropriate expression level for their gene...various promoters suitable for in vivo expression, and/or produce vectors to make mutant cell lines or transgenic...

.... This dual lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific... Dual lentivirus system with ubiquitous expression of sgRNA (top) and neuron-specific expression of dCas9... (i.e., how many sgRNA expression cassettes will fit). Cre-dependent expression of the SPH activator paired...the Day Lab, who developed neuron-optimized lentiviral vectors for in vitro and in vivo CRISPRa (Savell ...without inducing expression of the non-targeted variant. Moreover, isoform-specific expression of Bdnf resulted...physiology. The Day Lab’s neuron-optimized lentiviral vectors can be found here. High-throughput screening...differentially expressed genes between Bdnf I-overexpressing neurons and Bdnf IV-overexpressing neurons. Among...

...infecting cells with sgRNAiBAR library containing lentiviral vectors the iBARs can be detected by next generation...the stop cassette is removed, the effector is expressed. By truncating one of the recombination sites ...calcineurin-dependent response elements (CDREs) and drives gene expression. Because calcineurin is required for virulence...4X CDRE repeat. In addition to showing that this vector is suitable for flow cytometry and fluorescence...plasmids for purification of tTALE-eGFP and tTALE-mCherry. In comparison to intercalating fluorescent dyes... single molecules in real-time. Find plasmids expressing tTALE-FP Shin et al., Sci. Rep. 2019. https:/...

...engineering from a single lentiviral vector Mammalian Lentiviral expression of Cas9, dCas9, or dCas9-VP64... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 ...size and allow for expression of more sgRNAs in a single vector. sgRNAs are expressed as polycistronic ... pyogenes mCherry Luikart pDECKO_mCherry 78534 Mammalian hU6 none S. pyogenes Puro, mCherry Guigo BPK3079...gRNA expression vectors for insertion of custom gRNA target sequences. CRISPR...CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base...

...17408 Puro.Cre empty vector Cre, Puro resistance and miRNA expression PGK Lentiviral Jacks 17736 pOG231 ...sgRNA expression hSyn AAV Zhang 60820 pSECC Cre, Cas9 and sgRNA expression EF-1 alpha Lentiviral Jacks... Fuc-T2A-cre mCherry and Cre Lentiviral Luikart 66691 FUG-T2A-cre GFP and Cre Lentiviral Luikart 66692...Hirrlinger 107312 AAV-hSyn-mCherry-P2A-Cre-WPRE mCherry and Cre; expressed in neurons hSyn AAV Yang 107313...107313 AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA mCherry and Cre; expressed in excitatory neurons aCamKII...components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector Cbh Lentiviral Eisenhoffer 132551 AAV-U6-gRNA-TnT-Cre...Stoplight 2.4 Zsgreen is expressed in cells without Cre, mCherry is expressed with Cre Mammalian Hughes...

...endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90% in human cell...assess Cas9-PAGE editing with viral sgRNA targeting mCherry reporter (mChe), with co-incubation with assist...structured illumination microscopy in HeLa cells expressing COX8a-mStayGold. Scale bars 10 μm (main), 1 μm...developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene...bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters in green monkeys and cultured...cultured human neurons, with broader neuronal expression than AAV9. This new capsid will be a valuable ...N.C., Goeden, N. et al. Adeno-associated viral vectors for functional intravenous gene transfer throughout...

... you’ll need to express both the EB3N-VVDfast-iLID anchor module and the SspB-mCherry-p60 katanin module... activity. EB3N-VVDfast-iLID (anchor) and SspB-mCherry-p60 (katanin) are recruited to microtubules under...module, which are also available as lentiviral plasmids if you want to make a stable cell line. This design...specific area at the plasma membrane (Figure 2). Just express the plasma membrane-localized iLID along with your...Opto-RhoGEF design and activity. B) OptoTIAM (magenta) expressed in a monolayer of endothelial cells, showing cell...either CRY2 or CIB1 to your protein of interest and express the other separately, then use blue light to generate...probe GFP-PASS. B) Time series of HEK 293T cells expressing plasma membrane-targeted SuperPLDx30 (magenta...

...reporter vector expressing firefly luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter...2A-MCS-Hygro - Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter... are listed below: pBOBI-FLuc - Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - ...Retroviral reporter vector expressing firefly luciferase. pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter...reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro...reporter vector expressing firefly luciferase with neomycin resistance. Other Resources Protocols Bloom lab ...Viral Vectors 101: Pseudotyping Antibody Neutralization Response Against Pseudoviruses Expressing SARS-...

...in the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR... . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core (Link ...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Transducibility Some viral vectors are tested in vitro and in vivo for gene expression and/or function. These...Empty vector particles can be identified after negative staining and appear darker than full vector particles...

...Viral Vectors Lentivirus Oka Lab Lentiviral Vectors Baylor College of Medicine Gene Vector Core Lentiviral...series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Viral...and Adenoviral Vectors You may also like... Lentiviral Guide Adenoviral Guide Viral Vector Plasmids and ...Addgene Blog: Your Lentiviral Plasmid FAQs Biosafety Resource Guide Lentiviral Transfer Vectors Adenoviral Vectors... Protocol Lentiviral Vector ID Purpose pCDH-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter ...promoter when Cre is expressed by another vector pCDH-EF1-DIO-tdTomato 72254 Expresses tdTomato under EF-1 promoter...

...resistance. Wiederschain 36084 pLV-mCherry 3rd CMV driven expression of mCherry fusion. See plasmids 36083 and...Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G N/A Envelope VSV-G-expressing envelope plasmid...pCPRDEnv 2nd Packaging part of the FELIX vector system, expresses Gag-Pol and Rev Nolan 35617 pCAG-Eco N...MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG N/A Envelope VSV-G-expressing envelope...