Plasmids 101: Cre-lox
Type
Blog Post
...proceeds. One popular lox-stop-lox plasmid is from Tyler Jack's lab: Lox-Stop-Lox TOPO.
Cre-dependent ...tissue-specific cell types: Cre-lox recombination.
What is Cre-lox?
The Cre-lox system is a technology that...translocation event is generated at the loxP sites.
How can I use Cre-lox?
The Cre/lox system is a well-established...termination features with loxP sites on either side (often called a “lox-stop-lox” or “LSL” cassette) upstream...Among these are loxN (GtATACcT), lox2272 (GgATACtT), and lox511 (GtATACAT). These variant lox sites undergo...Cre and loxP, respectively. The FLP-FRT technology can be an effective alternative to Cre-lox, and has...embryonic lethality.
To facilitate the use of Cre-lox technology, transgenic mice have been constructed...