...Both loxP and lox511 are recognized by Cre but lox511 sites can only recombine with other lox511 sites... the identical loxP or lox511 sites (Figure 1, bottom). Since only one loxP and lox511 site will remain...with one loxP site and one lox511 site in the same orientation, and placing the additional loxP and lox511...binds loxP sites, while FLP binds FRT sites. When a DNA sequence is flanked by target sites (floxed) in ...one wild-type pair (loxP, black arrowheads) and one mutated pair (for example- lox511, white arrowheads)...sites, not with loxP sites. Alternatively, you could use a pair of loxP sites and a pair of FRT sites and... proceed either by first utilizing the loxP sites or the lox551 sites. Regardless, the first recombination...

...allele The design for a floxed conditional allele requires you to target 2 loxP sites to intronic regions...original design for creating a conditional allele, or floxed mouse, from Yang et al. requires two gRNAs targeting... and two repair templates in order to insert two loxP sites. Since two double-strand breaks are made, ...variety of other undesired outcomes, including one loxP site insertion and one NHEJ-repaired indel, often... - 0.8 kb apart. In the example below, we’ll add loxP sites for conditional deletion of exon 6 of Syt1...homology on each side. For optimal repair, place the loxP sites precisely at the cut site for each guide. ...efficiency of Easi-CRISPR for simple knock-ins and floxed models varies from 8.5-100% of pups born, with ...

...technology (Cre/loxP) to remove the donor cassette. While this method can be effective, a loxP site is left...Grégoire and M. Kmita, “Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides...

...pAAV-mDlx-NLS-mRuby2 112677 AAV1, AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 27056 AAVrg pAAV-Ef1a-DIO EYFP ... mCherry 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 50457 AAV2 pAAV-hSyn-DIO-EGFP ...

...easy to remove after injection, they added flanking loxP sites and a heat-shock inducible Cre to generate...schematic below.) After self-excision, the resulting loxP scar is located in this synthetic intron, and only...

...GABAergic interneurons. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5 and 112677-AAVrg): The...)-mCherry (26976-AAV5) pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5) Calcium Sensors pGP-AAV-syn-jGCaMP7b-WPRE...

... of gene-specific 1 kb homology arms flanking a FLOXed neoR gene. pAAV-SEPT-Acceptor, the preferred vector...Then, if necessary for subsequent experiments, the floxed neoR gene is removed via infection with adeno-cre...

...Lab used this backbone to target a CAG-driven loxP-stop-loxP cassette into the Rosa26 locus, allowing for...Commonly used sequences, including fluorescent tags, a floxed Cbr-unc-119 selectable marker, and “connectors”...

... AV-1-PV2699 100030-AAV1 pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40 Lawrence Cohen AV-1-PV3509...Svoboda AV-1-20297P 20297-AAV1 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Karl Deisseroth...Deisseroth AV-1-20298P 20298-AAV1 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA Karl Deisseroth AV-1-20938M...Deisseroth AV-5-20297P 20297-AAV5 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Karl Deisseroth...Deisseroth AV-5-20298P 20298-AAV5 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA Karl Deisseroth AV-5-20938M...Svoboda AV-9-20297P 20297-AAV9 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Karl Deisseroth...Deisseroth AV-9-20298P 20298-AAV9 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA Karl Deisseroth AV-9-20938M...

...Combinations of classical genetic tools, for example Cre/loxP or Flp/FRT, with AAV vectors are common approaches...

...stochastic multicolor labeling, Brainbow used the Cre-loxP system to express one of the three fluorescent protein...

...pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double floxed human muscarinic receptor 4 (Gαi-coupled) DREADD...

...popular method uses Cre recombinase on transgenic flox animals to introduce modifications at the DNA level...

...these needs is the lentiviral-based expression of floxed dCas9 so that it may later be excised by Cre recombinase...

...nonfluorescent (Halo, SNAP) tags, a selectable marker (floxed Cbr-unc-119) for easy screening of the insertion...

...N. K., Lee, C. K., Abu Bakar, S., AbuBakar, S., Hollox, E. J., & Boon Peng, H. (2015). A comparison of...

...the cap gene. In this special CREATE cap plasmid, loxP sites flank the downstream polyA sequence. When ...

...-made AAV vectors which can be used in numerous floxed animal species for behavioral tests.    Forced ...

...(1979) Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method...

...Also, drugs like BM Cyclin, fluoroquinolone ciprofloxacin, ciprobay, zagam, baytril, tetracycline, etc...