Special Delivery: Fluorophore Targeting for FRET Studies
Type
Blog Post
... dyes are now commercially available as Lumio Green and Lumio Red from Invitrogen (now ThermoFisher Scientific...Acceptor
Tag
R0 (Å)a
Range (Å)b
Applications
CFP
Lumio Green
Tc: CCPGCC
50
40-72
Intact Cells
Cy3NTAc...Cy5NTAc
32
25-45
GFP (fluorescein is similar)
Lumio Red
Tc: CCPGCC
48
38-70
Intact Cells
Cy3NTAc
Poly...permeabilized cells
Cy5NTAc
43
34-62
YFP
Lumio Red
Tc: CCPGCC
54
42-78
Intact Cells
Cy3NTAc
Poly...