Special Delivery: Fluorophore Targeting for FRET Studies
Type
Blog Post
Published
July 19, 2016, 2:30 p.m.
... dyes are now commercially available as Lumio Green and Lumio Red from Invitrogen (now ThermoFisher Scientific...Acceptor
Tag
R0 (Å)a
Range (Å)b
Applications
CFP
Lumio Green
Tc: CCPGCC
50
40-72
Intact Cells
Cy3NTAc...Cy5NTAc
32
25-45
GFP (fluorescein is similar)
Lumio Red
Tc: CCPGCC
48
38-70
Intact Cells
Cy3NTAc
Poly...permeabilized cells
Cy5NTAc
43
34-62
YFP
Lumio Red
Tc: CCPGCC
54
42-78
Intact Cells
Cy3NTAc
Poly...