...efficiency than in iPSCs, but with the caveat of mosaicism. To circumvent this problem, they co-injected ...Indeed, they observed that 41/42 embryos were non-mosaic at 3 days post fertilization. Since Ma et al. designed...term ‘editing.’ While repair using endogenous chromosomal DNA is appealing for heterozygous mutations, ...needed to define whether off-target editing or mosaicism would occur. Although Ma et al. looked at various...this detailed analysis is not possible with IVF, as most of the cells are needed for proper development. .... Ma et al.’s experiments showed a 3% mosaic rate, and this rate would need to be much, much lower before...

...fast-tracked development of diagnostics. Mostly transmitted by Aedes mosquitoes (aegypti and albopictus) and, aided...virus had received little attention and, for the most part, had been associated with low morbidity and...conducive to the spread of the virus by the Aedes mosquito (Messina et al, 2016). We quickly assembled a ...true team effort and many late nights, not unlike most research, but the urgent need for the test did catalyze...community when we needed additional expertise. Perhaps most striking is that there were seven institutions involved...

... be the most common poster size, but this can vary as well.  Sections of your poster In almost all scientific...be as concise as possible, communicating only the most important results to your audience. With a poster...Adobe also offers free poster templates, although most are vertical.  Large central box. Another commonly... of this style is that it will display your data most prominently, making it the star of your presentation...navigating the poster session Find tips to make the most of your conference attendance Read all of our blog...

...preparation and day-of game plan will ensure you have the most beneficial scientific conference experience possible...your poster as a visual aid, not a crutch. Even the most prepared of us can find ourselves talking to our...navigate questions Answering questions is arguably the most difficult part of a poster session, but with a little...which posters you are interested in viewing. For most larger conferences, poster titles and abstracts ...to the session, so bookmark which posters are of most interest to you a few days in advance. If you don...website. Time permitting, you can go back to the most interesting and popular posters at the end of the...

... to correct only a minority of those variants in most cell types. A new technique from David Liu’s lab...binding site, increase processivity, or improve thermostability. What came out on top? The Cas9 nickase fused...efficiency of HDR is relatively low, meaning that most Cas9-induced DSBs are repaired by non-homologous... end joining. As a result, Cas9 treatment causes most products to be indels while the efficiency of HDR...Leukemia Virus reverse transcriptase increase thermostability through tighter binding to template-primer....Generation and characterization of new highly thermostable and processive M-MuLV reverse transcriptase ...

...retroviruses have been developed into some of the most widely used tools in molecular biology. “Retroviruses...of their more complicated genomes. Here, we will mostly use the gamma-retroviruses as a simple spokesmodel...molecular biologists working with vectors are, in most cases, interested in transferring genes to mammalian...double stranded DNA is integrated into the host chromosome, and this is where viral vectors and natural ...express. In the case of a natural virus, this new chromosomal locus is considered “proviral DNA” (Rowe et al...Resources on addgene.org Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide ...

...change, the safety of genetically modified organisms (GMOs), the absence of a link between vaccines and autism...Association for the Advancement of Science) believe GMOs are safe for human consumption in comparison to ...active research scientists believe climate change is mostly due to human activity while much less of the general... of the word ‘fact’. Policy must be based on our most accurate interpretation of the world, and this interpretation...the following subjects: Climate change Vaccines GMOs Transgender issues Reproductive health Immigration...paired with long-term science outreach, may have the most profound impact (15). Diversity and inclusion ...

... expect: Search bar The biggest change is to our most frequently used feature: the search bar. We’ve changed... easy-to-use depositing guide, with links to our most helpful depositing articles. If you’re depositing...Figure 2: The new deposit materials bar New menu Most common activities can be done straight from the ...educational resources page, and previews for our three most recent blog posts. Finally, you’ll find everyone...discover as you browse. You may find one of your most frequently used buttons stands out better on the...

...questions can elicit the most valuable insights you will get from the interaction. Most people like to help...help us learn about the nonacademic sphere where most of us will be working.  Check out Joanne's Reddit... lists of questions on the internet. To make the most of the opportunity, use a written list and take ...stories. Thanks to the Benjamin Franklin Effect, most people actually enjoy helping (just ignore the people...

...candidate at the University of Cambridge. One of the most important steps in the CRISPR experimental process... a variety of options for CRISPR genotyping. The most common options include mismatch cleavage assays,...genome and sgRNA. This is a good starting point for most researchers as it provides a list of putative off-target...effects in the clinic. NGS-based methods provide the most complete information profile regarding putative ...comprehensive list of off-target editing events. Two of the most common cell-based methods are genome-wide, unbiased...using just genome-wide unbiased methods. However, most investigators use single cell clones for in vitro...

... job opportunities in academia - the main reason most people enter postdoctoral training. Similarly, there...example). This blog post is about how to get the most out of your postdoctoral training experience. If... biomedical postdoc fellowship lasts five years. Most universities and institutes annually advertise for...you how to write as well as mentor. Yet, even the most dedicated mentor is not always available for mentees... you or the audience. Ask yourself: What is the most exciting story I can tell with my data?  Can I make...this blog add up to a substantial amount of time- most of your waking hours. This is doable and it's a ...

...your experiments, I've described below some of the most frequently requested vectors in our repository and... consider as you make your choice. The first and most important thing you need to know is your expression...or a fusion protein. Mammalian vectors One of our most popular mammalian expression vectors, c-Flag pcDNA3... YGOI in an isolated, controlled environment. In most cases bacterial expression vectors are inducible... We have a variety of other types of vectors for most applications used to study gene function. From making...

...replicate the plasmid in addition to its own chromosomal DNA, costing additional resources to maintain... have also been used to disrupt genes at the chromosomal level. Scientists introduce an antibiotic resistance...Tips and tricks from the bench Use fresh stocks. Most antibiotics are stable in powder form, but quickly...and avoiding repeated freeze/thaw cycles will keep most antibiotics viable for at least 6 months. Ampicillin...Ampicillin and can be used in place of Ampicillin in most applications. Antibiotics vary in their sensitivitly...

...unusual ploidy and are considered hypotriploid: most chromosomes are represented by two to three copies, but...but several have four. No Y chromosome is present in this line, making it genetically female. HEKs are...suspension. Of the many derivatives of HEK-293, the most commonly used line expresses SV40 large T antigen...transcriptome studies of the cell line have determined it is most likely adrenal in origin. (Anatomy refresher: the...

...which is the primary distinction between two of the most frequently used yeast strains: S. pombe (fission...pombe or S. cerevisiae. Actually, it’s neither. The most commonly used yeast by biotech and pharmaceutical...Facultative anaerobe Obligate aerobe Number of chromosomes 16 3 4 Preferred carbon source Glucose...media buck - it grows at higher cell densities than most of its counterparts, yielding higher production ...lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic for human...

...Curious which viruses researchers have found the most useful so far? We crunched the numbers on our Viral...Service (and then we crunched them again) to find the most requested lentivirus and AAV of 2016. The top viruses...drumroll please)... Top Lentivirus: lentiCas9-Blast Most of Addgene’s lentiviral inventory is based on CRISPR...non-CRISPR related viral preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your...Gi)-mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs from Bryan...

...students. I believe that communication is the single most important skill that scientists need to succeed ... that you can succeed in communicating. Even the most rigorous, insightful, and novel scientific research... skill in science communication over time.  Like most skills, great science communication comes with practice...process of writing papers and proposals.  To get the most out of these interactions, ask yourself how every...you intended. 3. Join the scicomm community Like most aspects of science, communication isn't easy.  But...

...here are the basic requirements of use. First, and most obviously, you need a non-canonical amino acid, ...codon used is often one of the three stop codons ­– most commonly the UAG codon – in some systems, rare codons... with systems and ncAAs that were, in practice, mostly accessible to experts. That many GCE technologies...accessible to your lab. The solution GCE provides almost endless possibilities for researchers, from selective...own labs. They provide resources, knowledge, and most importantly, validated protocols (using readily ...

...Congratulations Mark Mellett! Siddharth Prakash: X chromosome genes involved in Turner syndrome Turner syndrome... a developmental disorder where one of the X chromosomes is partially or completely missing in women. ... cells from women who are missing an entire X chromosome (TS iPSCs), the lab observed abnormal cell migration...developmental defects. Eight genes from the X chromosome are expressed at significantly reduced levels...reagents will facilitate our aims to correct X chromosome gene dosage in TS cells as a first step to reverse...

...As a scientist, perhaps the most important part of your research is communicating the results of the ...different amounts of access or feedback. One of the most significant shifts in scientific communication is...part of their scientific communication needs. The most often-cited reasons researchers might not post a...biases or errors, with a lack of transparency in most review processes. Preprints and their feedback, ...discussed and addressed by the scientific community. Most preprints are eventually published in journals after...