...Submit your grant early! First off, and perhaps the most important bit of advice I can suggest, is to give... your application and give you feedback. They’ve most likely been through the process themselves many ...Originality is essential for grant success The two most important things reviewers will be looking for in... worth knowing? This can sometimes be tricky, as most of our research builds on ideas and findings from...explanation of your research for non-scientists. Most fellowships are funded by the general public (either...

...service interaction - one that gives our customers the most utility in the least amount of time. Though I now...accumulated wisdom so that you, the customer, can get the most out of your email or phone call. Though my experience...times, I’m pleasantly surprised to discover that the most crucial information about a product is printed right...asked by customers (and I expect this is the case at most companies). Always go back to the source. Although...Following the tips above should help you get the most out of your interaction.   Resources on the Addgene...

...antagonistic and impolite, Complainers, who spend most of their time moaning about their troubles, Silent... are a few principles that will help you resolve most conflicts, even with difficult people. If I had ... Put important agreements in writing One of the most frequent sources of conflict is miscommunication...or just an inexperienced manager, you can resolve most conflicts if you communicate your ideas assertively...www.FinishYourThesis.com.   Additional Resources Getting the Most Out of Supervisor Meetings by Joanne Kamens on  ...

...fragments together in a one-tube reaction. Even though most MoClo reactions use Golden Gate cloning, not all...are defined DNA sequences with specific functions. Most individual parts have characterized activities with... with predictable behaviors. While a gene is the most obvious example of an individual part, this category...review a list of MoClo applications. Genetic circuits Most things in biology are not so simple, and engineering...delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled and delivered virally...

...interesting. If people could be against GMOs as food but support GMOs that result in a new utility, was there...presence. They are genetically modified organisms, and GMOs have a bad reputation. The public is can be fearful...comments were more often along the lines of “I hate GMOs, but what you are doing makes sense. I can support...will need, and the business training to make the most of it. It has also allowed us to really analyze ...

...capable of spreading through wild populations. Whereas most genomic changes impose a fitness cost and are eliminated...heterozygotes, the endonuclease cuts the wild-type chromosome and its own gene is used as a repair template...experiments attempting to build gene drives in tropical mosquito vectors of diseases such as malaria and dengue...don't have resident populations of the relevant mosquito species. Reproductive confinement involves working...Control of Insect Populations: I. Cage Studies of Chromosome Replacement by Compound Autosomes in Drosophila...

...hood is in use. Wipe the hood with a disinfectant (most people use 70% ethanol, but you can also use Incidin...contamination. This sounds rather trivial, but often solves most problems before they occur. With proper planning...contact with the cells should already be at 37 °C. Most labs have a water bath constantly at 37°C for this... to form a routine. Twice a week often works for most fast-growing cell lines (such as HEK293, which multiplies... for experiments requiring reproducible results. Most cell culture media contain the pH indicator phenol...

...bacterial host cell is almost always the answer. E. coli is definitely one of the most popular hosts for protein... that make up the primary structure of proteins. Most proteins are then modified via an array of post-...post-translation modifications for proper protein function. Most DNA constructs designed for mammalian expression...used for protein expression, S. cerevisiae, is the most reliable and frequently used species due to its ...

...plant research here and validating them, it made the most sense to us to enable other people to do that too...expression in plant cells. Sylvester and I chose the 96 most useful things for day-to-day life in a plant lab...will work in any dicot, but they were developed mostly with model plants in mind, Arabidopsis and Nicotiana...with MoClo? Patron: The parts will be familiar to most plant scientists. It’s really a matter of choosing.... It’s also a great communication tool for work. Most Sainsbury scientists are active on Twitter. Sometimes...

...over time that the most successful computer languages are free to use. Of the 15 most popular computer ... operators are so useful they are implemented in most materials that have been engineered. For example...it's free to use and that tends to be true about most successful human-to-human languages. Who owns computer-programming...bioeconomy it's less clear, but the way I'd leave it is mostly to the imagination. The question is: what can you...

...for 10 years. In that time, scientists have used almost all available approaches for generating iPSCs. ...cut and paste” mechanism between a vector and chromosomal DNA. PB has a cloning capacity of ~9-14 kb. This...assists in tethering the vector to the cell’s chromosomes. With the addition of a selectable marker, this...EBNA1 plasmid to be maintained as a stable extrachromosomal piece of DNA. Reprogramming efficiency is ...three germ layers. Choosing a reprogramming method Most iPSC research falls into two categories: 1) studies...PMCID: 5072705. 2. Sommer, Cesar A., and Gustavo Mostoslavsky. "The evolving field of induced pluripotency...

...they lead Zimmer to one of his most critical observations: “Today the most successful scientists have outgoing...is arguably the most subjective chapter of the book, but that’s unlikely to be foremost in readers’ minds...memory lane for me. A brief window in time “The most obvious future consequence of the undermining of...

...plasmid-based expression of Cas9/gRNA and can be used for most of the current genome engineering applications of...be amenable to plasmid based methods. One of the most common techniques for delivery of RNPs is electroporation...up to the researcher to determine which method is most appropriate for a given experiment. Considerations...that may arise when using plasmid based systems, most notably the ability to perform CRISPR studies in...PMC5253684. 5. Harrington, Lucas B., et al. "A thermostable Cas9 with increased lifetime in human plasma...

...these vectors. Read on to find the answers to our most frequently asked lentiviral questions. This useful...Second-generation vectors are likely sufficient for most experiments; however, the 3rd-generation packaging...answer the questions those of us at Addgene get asked most often: Q1: Are retroviruses and lentiviruses the...plasmids replication competent? What is SIN? A6: Most (if not all) Addgene transfer plasmids are replication-deficient...ClonTracr Library Resources on Addgene.org Addgene's most popular lentiviral plasmids Overview of Lentiviral...

...personal 10 commandments for making it all work – most of the time. #1 Thou shalt find enjoyment in your... to 6 and 8 to 10 or whatever works for you, but most days go home at the same time. Use time management...to save a step! There is more than one way to do most tasks – try to resist the impulse to control by ...also have fun I’m really busy, but I go to the gym almost every morning. Of course, this is more than just...Building your plan to live a good life may be the most challenging assay you will ever have the opportunity...

...waiting for an indication of what (most of) Harvard, Johns Hopkins, (most of) MIT and the University of Colorado...having a major effect on scientists within academia, most particularly postdocs working in the U.S., whose...from the NSF’s 2014 Graduate Student Survey data). Most institutions suggest that postdocs should be paid...mid-career scientists/junior faculty will all be most/least likely to have to get rid of postdocs; that...

...formation by most, if not all, of the FP fusions. Our moxFPs currently represent one of the most inert solutions...function in the environment where it will localize [4]. Most investigators are surprised to learn that FPs are...environments. However, approximately 40% of human (and most eukaryotic) proteins localize to chemically distinct...assays. However, there has been a pervasive view that most FPs in a cell will fold and that the fluorescence...

...then choose the most efficient approach for your own system. The simplest and the most popular one is ...and others. CFP-YFP is still one of the best and most used pairs to measure FRET. The table below lists...that have a similar brightness - even though in the most popular pair, CFP has five-fold less brightness ...FLIM has been developed more recently and is the most rigorous method for measuring FRET. FLIM measures...

...researchers are optimistic that this can be done for most, if not all, of these new experimental species. ...to create a different protein. Did you know that most cephalopods die after they lay their 1 clutch of...the smallest photosynthetic cell in existence, but most abundant cell on earth (twice the human biomass)...Manihot esculenta (Blake Meyers) Cassava is the fifth most grown crop in the world so it feeds a lot people...arthropod germline by injection into adult female mosquitoes. Now they are trying to replace embryo injection...

... primarily office based, and email/phone are the most popular ways to communicate with customers. Online...to run experiments with/for them. Though not the most prestigious role in a company, Technical Support...insights into how other scientists think and work. Most certainly there won’t be a shortage of amusing reports...questions. Identifying and solving problems are the most rewarding parts of the job. Solutions may also be...technical support for the long term). First and foremost, technical support gives you the opportunity to...