...decrease in red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe that...molecule of GFP-tagged LC3 for every one molecule of RFP-tagged LC3∆G. Like the unmodified LC3, GFP-LC3 is conjugated...autophagy biosensor is the GFP-LC3-RFP-LC3ΔG probe. It’s similar to the mRFP-GFP-LC3 Tandem Fluorescent Protein...Addgene! mRFP-GFP-LC3 tandem fluorescent protein This autophagy biosensor contains mRFP and GFP fused to...chain 3B, or LC3. LC3 is a commonly used marker for measuring autophagic flux. mRFP-GFP-LC3 functions as...decrease in autophagic flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky, Daniel...same mechanism as Rosella: GFP is quenched in acidic environments, while mRFP is relatively stable and ...

... Gerlich 25999 LV-GFP Chromatin H2B GFP Elaine Fuchs 11680 H2B-GFP Chromatin H2B GFP Geoff Wahl 21210 ...Autophagosome LC3 EGFP Tamotsu Yoshimori 24920 pEGFP-LC3 (human) Autophagosome LC3 EGFP Toren Finkel 14435...Rubinsztein 11546 EGFP-LC3 Autophagosome LC3 EGFP Karla Kirkegaard 61205 pCMB-AUTr Autophagosome LC3(ATG8) mCherry...Autophagosome LC3 mCherry, GFP Anne Brunet 40827 mCherry-hLC3B-pcDNA3.1 Autophagosome LC3 mCherry David Rubinsztein...14437 mRFP-Rab5 Early endosomes RAB5A mRFP Ari Helenius 61801 GFP-Rab4B Early endosomes Rab4B AcGFP Gia ...61803 GFP-Rab7A Late endosomes Rab7a AcGFP Gia Voeltz 12605 GFP-rab7 WT Late endosomes RAB7 GFP Richard...components. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI 110060 FUW mCherry-GFP-LC3 Autophagosome...

... Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service: AAV Biosensors...troponin C Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin Protein) for imaging...FLAMP1 and FLAMP2 Chu Lab FLAMP Plasmids Jun Chu cGMP (cyclic GMP) FlincG GFP-based cGMP sensor Differential...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ...autophagic flux by pH-sensitive fluorescence (pMRX-IP-GFP-LC3-RFP) An Autophagic Flux Probe that Releases an ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Autophagy Autophagosome maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation process...