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We narrowed to 9 results for: nuclear bfp

Showing: 1 - 9 of 9 results
  1. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ... range).  By mutating GFP, the variants blue FP (BFP), cyan FP (CFP), and yellow FP (YFP) were derived...will pass blue light to the detector/camera, then BFPs are of no use to you. When using more than one FP...imaging technologies to study gene expression, nuclear localization, and dynamics” Curr. Opin. Cell. Biol... light. Photostability can be as short as 100ms (EBFP) or as long as 1 hour (mAmetrine1.2). However, for...
  2. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...chromosomes and how these arrangements may affect nuclear processes. The latest of these visualization tools...that each recruits a different fluorescent protein (BFP, GFP, or RFP) to the genomic loci of interest. Pairs...
  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...mCherry-LaminA-C-18 Nuclear envelope LaminA1 mCherry* Michael Davidson 98830 mTurquoise2-LaminB Nuclear envelope ... Snapp 68126 ERoxBFP Endoplasmic reticulum ER retention signal oxBFP Erik Snapp 49150 BFP-KDEL Endoplasmic...Gia Voeltz 49151 mito-BFP Mitochondria Mitochondrial targeting signal (COX4) TagBFP Gia Voeltz 55102 mCherry-Mito...) Rab5 mCherry Gia Voeltz 49147 BFP-Rab5 Endosomes (early) Rab5 TagBFP Gia Voeltz 61802 GFP-Rab5B Endosomes... James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc Endosomes (early) Rab5a TagBFP James Johnson 13050 DsRed-Rab5... mCherry Gia Voeltz 49154 BFP-Sec61 beta Endoplasmic reticulum Sec61 TagBFP Gia Voeltz 231776 pCMV-mGold2t-Calnexin-N...James Johnson 79805 pTag-BFP-C-h-Rab11a-c-Myc Endosomes (recycling) Rab11a TagBFP James Johnson 79800 pTag-RFP-C-h-Rab4a-c-Myc...
  4. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...pDAS12222_U6-pegRNA-BFP Mammalian hU6 pegRNA BpiI for pegRNA, Esp31 for PBS-RT No BFP Ervin Welker Return...containing RT optimized for human codons, additional nuclear localization signals, and two mutations in Cas9...
  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61 beta BFP-KDEL Rtn4a-GFP (tubular ER) Microtubules...-Rab5 BFP-Rab5 GFP-Rab5B Vacuolar & Budding Compartment GFP-Rab4B Mitochondria mito-BFP mCherry-Drp1...actively shuttled out of the nucleus due to the nuclear export sequence. Treatment with rapamycin induces...dCas9s with one of three fluorescent proteins (GFP, BFP and mCherry), the authors were able to visualize ...genome and to determine, for instance, the intra-nuclear distance between loci on different chromosomes....
  6. Brain Initiative Collection

    Type
    Collection
    ...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons...pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons...
  7. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...including mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs ...luciferase (NanoLuc), fluorescent proteins (TagGFP2, TagBFP, TagRFP, and T2A-TurboGFP-PEST), and small epitope...
  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...using selection by Puromycin, Zeocin®, or nuclear expression of BFP2.     Figure 1: CROPseq-multi ...
  9. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Guide and the Michael Davidson FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid...myc-nuc - pEntry vector that adds a C-terminal nuclear localization signal pGADCg - Gateway yeast expression..., T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector with C-terminal mTagBFP2 tag pNIC-GST-TEV-...plasmid expressing mCherry pBad-EBFP2 - Bacterial expression of EBFP2 blue fluorescent protein 35S-eGFP-nosT...mCherry selectable marker pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1...
Showing: 1 - 9 of 9 results