Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 48 results for: pSC

Showing: 1 - 20 of 48 results

  1. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...available approaches for generating iPSCs. The generation of iPSCs is relatively simple in concept: ectopically...differentiation has been linked to tumor formation in iPSC-derived and iPSC-transplanted mice. Cre-deletable or Tet-...these experiments. Assays for iPSCs Characterization Once generating iPSC clones, it’s important to determine...chimaeras to produce all-iPSC derived offspring demonstrates the ability of the iPSCs to form functional germ... mouse iPSCs, tetraploid complementation, involves injecting tetraploid blastocysts with iPSCs and measuring...The field of induced pluripotent stem cells (iPSCs) has been around for 10 years. In that time, scientists...Key Features of Different Methods for Generating iPSCs. One important note: in this table, reprogramming...

  3. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...revert back to a pluripotent stage (iPSC formation) [2, 3]. The iPSCs then proliferate and redifferentiate...list of methods and the species they were used in. iPSCs have now been generated from many different types...isolated somatic cells that have been reprogrammed to iPSCs successfully [10-12]. The next stage of reprogramming...reprogramming consists of redifferentiation of iPSCs into the cell type of choice. This step is sometimes also...growth factors are used to control the cell fate of iPSCs and differentiate them into different cell lineages...have been successfully differentiated from human iPSCs. Below is a list of some of these cell types [13...Epigenetic marks Complete removal to convert to iPSCs and re-establishment after Partial rearrangement...

  4. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    ...generating iPSCs iPSCs and ESCs closely resemble each other in many aspects. Like ESCs, iPSCs have a high...induced pluripotent stem cells or iPSCs. Induced pluripotent stem cells iPSCs are stem cells that are obtained...similar to ESCs [11].  While iPSCs and ESCs have similar functional utility, iPSCs bypass the transplantation...four embryonic genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated from mouse fibroblasts in 2006... labs showed the successful generation of human iPSCs from dermal fibroblasts via retroviral and lentiviral...universally to generate many adult cell types. Like ESCs, iPSCs can differentiate in vitro into derivatives of all... form embryoid bodies, and form teratomas [11]. iPSCs also possess similar morphologies, growth properties...

  5. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    ... therapeutic applications of iPSCs. The generation of human iPSCs (hiPSCs) now provides an opportunity...0.001% to nearly 0.1% [7]. In terms of hiPSC colonies, nearly 16 hiPSC colonies can now be obtained from just...differentiated cells into induced pluripotent stem cells (iPSCs) by the addition of reprogramming (or “Yamanaka”... cells have been probed for their suitability in iPSC programming spanning from fibroblasts and keratinocytes...blood present many benefits for the production of iPSCs: Blood is easily and routinely drawn from patients...unique epigenetic signatures that are closer to hiPSCs than age-matched fibroblasts [3]. These advantages...viral protein EBNA-1. Methods for reprogramming hiPSCs from PBMCs While the method of choice for reprogramming...

  7. iPSC Neurodegenerative Disease Initiative Plasmid Collection

    Type
    Collection
    ... by the iPSC Neurodegenerative Disease Initiative (iNDI) here.... Depositor Collections iNDI iPSC Neurodegenerative...Research Neurodegeneration Research Collection The iPSC Neurodegenerative Disease Initiative (iNDI) project...collection at Addgene. Figure 1: Schematic of iNDI iPSC line generation, phenotyping, and distribution efforts...-tagged gene variants or to differentiate human iPSCs into other cell types, as noted in the purpose field...

  8. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...replication. But pSC101 requires a third component: RepA, a protein encoded by pSC101 itself. RepA binds...mechanisms of stringently controlled plasmids pSC101 and ColIb-P9. pSC101 copy number is regulated by iterons. ...mechanism of plasmids under stringent control, such as pSC101. DNA replication and regulation in bacteria Before...balance the total plasmid number.  Let’s consider pSC101. This plasmid is stringently controlled and has...the bacterial chromosome. Since replication of pSC101 depends on host-encoded proteins, it can be regulated... and IHF. RepA also controls the copy number of pSC101 through a negative feedback cycle. As with DnaA...expression and thereby the overall replication of pSC101 (del Solar et al., 1998). If that’s not enough ...

  9. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ... that I might be able to attend the 10 Years of iPSCs Symposium earlier this year, I jumped at the chance...possible to generate induced pluripotent stem cells (iPSCs) capable of forming nearly all cell types in the...so quickly, citing the first clinical trial with iPSCs with his collaborator Takahashi in 2014 – just 8...or non-existant). On the very first night of the iPSC symposium, a panel of thought leaders in the field...stem cells, and the difficulty of sharing banked iPSCs across international borders. While questions of... Learn about Efforts to Fight Rare disease with iPSCs Read About Addgene's Outreach Efforts in the Microbiology... ipsc...

  10. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ... (Table 1). Additionally, hiPSCs and mouse BM cells still behaved like iPSCs and BM cells after Nanoblade..., such as human induced pluripotent stem cells (hiPSCs). Nanoblades, a new CRISPR delivery method developed...delivery methods, but the researchers was able to edit hiPSCs, mouse bone marrow (BM) cells, primary human hepatocytes...type Gene targeted by guide Editing efficiency hiPSCs  EMX1  67% Mouse BM cells from GFP transgenic...

  11. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...changes in human induced pluripotent stem cells (iPSCs). The CRISPR/Cas9 com­plex is very efficient at ...system to introduce disease-causing mutations in iPSCs (or correct them back to wild type), differentiate...those of you involved in the tedious business of iPSC clone picking, a 100-fold decrease is the difference...-Maximilians-University Munich. His lab develops iPSC-based models of neurodegenerative and neurovascular...mechanisms of Alzheimer’s disease by developing novel iPSC-based model systems.     References 1. Cong, Le,...

  12. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...impact of KLF4 N-terminal variants on iPSC generation Deriving iPSCs (induced pluripotent stem cells) through...reprogramming somatic cells. A common method for generating iPSCs to express the transcription factors from a polycistronic...libraries Transcription factor fusions elucidate iPSC reprogramming mechanism The Ptashne lab was the...activation domain fusion increases the efficiency of iPSC reprogramming while the HP1 fusion eliminates reprogramming...28813671 Listen to the podcast segment on modulating iPSC reprogramming  Regulation of telomere length with...

  13. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...women who are missing an entire X chromosome (TS iPSCs), the lab observed abnormal cell migration and contraction...the lab will express these candidate genes in TS iPSCs to identify whether any rescue cell migration and...generate deletions of candidate genes in euploid iPSCs. “The BeHEARD Addgene reagents will facilitate our...observed.” Using genetically-encoded biosensors and iPSC-derived cortical neurons from patients with GNB1...

  14. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...mutations corrected by CRISPR/Cas9 in wild-type human iPSCs. Image modified from Le et al., 2019. Gene editing...mutation into the MECP2 gene in wild-type human iPSCs and then to successfully repair the mutation (Le...such as CRISPR and RNA editing or the induction of iPSCs, are available in the Resource Center. Researchers...cell line repositories including patient-derived iPSCs, MECP2 animal models, tissue banks, Rett databases...

  15. Hot Plasmids - August 2020

    Type
    Blog Post
    ...Nanobody purification CRISPRi and CRISPRa system for hPSCs The CRISPR corner New from the viral service Faster...these variations in human pluripotent stem cells(hPSCs), Lindy Barrett’s lab at the Broad Institute developed...and the dCas-VPR repressor are introduced into the hPSC genome using AAV1 and contain an EGFP reporter. ... dCas9-KRAB activator or dCas9-VPR repressor in hPSCs is also driven by a doxycycline inducible promoter...

  16. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...* C Stringent pACYC ~10 p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300-500 ColE1 (...slightly different but well known derivatives), pSC101, R6K, and 15A. Not all origins of replication are...

  18. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...Learn more about induced pluripotent stems cells (iPSCS) and reprogramming and direct reprogramming Explore...delivery of reprogramming factors for generating iPSCs Learn how fluorescent proteins help researchers ...

  20. Editor's Choice, September 2016

    Type
    Blog Post
    ...here: Four Things I Learned at the 10 Years of iPSCs Symposium Tips for Writing a Good Cover Letter Multiple...
Showing: 1 - 20 of 48 results