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We narrowed to 13 results for: photoactivated gfp

Showing: 1 - 13 of 13 results

  1. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...reported was PA-GFP. PA-GFP was derived from Jellyfish (Aequorea victoria) GFP (wtGFP) by mutating Threonine...mutation in PA-GFP generates a chromophore population that is more neutral, causing PA-GFP to display almost... for vanishing, and pa, for photoactivation) (6). Dronpa is photoactivated by blue wavelengths of light...example, by engineering PA-GFP tagged to a mitochondrial matrix protein (mito-PAGFP) the Youle Lab was able...H., and Jennifer Lippincott-Schwartz. "A photoactivatable GFP for selective photolabeling of proteins ...Christina, Steven M. Markus, and Wei‐Lih Lee. "Photoactivatable GFP tagging cassettes for protein‐tracking studies...Imaging Catch Up on Your GFP Background Resources at Addgene.org Find Photoactivatable FPs in Empty Backbones...

  2. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ... to track where GFP labelled B cells were in mounted slices of these organs. These GFP labelled cells ...enabled by fluorescent proteins The optimization of GFP and its derivatives by the late Roger Tsien changed...stimulus. Using an epifluorescence microscope and GFP fusion proteins controlled by promoters specific ...the immune lineage, scientists could easily track GFP expressing cells. For example, by harvesting lymph... the infiltration of neutrophils expressing LysM-GFP (green) into a draining lymph node after infection...proteins. Additional fluorescent protein tools Photoactivable fluorescent proteins (proteins that fluoresce...movement by marking cells at a specific time. Photoactivable fluorescent proteins, for example, have been...

  3. Hot Plasmids - October 2022

    Type
    Blog Post
    ...frequencies with (magenta) and without (black) photoactivation of HcKCR1 in mouse neurons. D) Membrane voltage...mouse neuron without (top) and with (bottom) photoactivation of HcKCR1. Images adapted from Govorunova et...Addgene’s western blot for the myc-tagged protein GFP-smFP-myc expressed from Plasmid 98926. See the Applications...

  4. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...chemistry! The discovery of green fluorescent protein (GFP) sparked a renaissance in biological imaging. Suddenly...small-molecules: red-shifted spectra, ion sensitivity, photoactivation, etc. These impressive advances lead to an ...an obvious question: In this age of GFP and its ilk, why should cell biologists talk to chemists? One ...brighter conjugates, higher fluorogenicity, photoactivation etc.—can further increase the photon budget...properties, exhibit high fluorogenicity, are photoactivatable, and function in vivo.* Final thoughts The...

  5. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post
    ...Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable fluorescent...(dron= a ninja term for vanishing, and pa= photoactivation) domains on either side of the active site ...Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent Proteins Control Protein Expression...

  6. Bacterial Expression Systems

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    Collection
    ...many different restriction enzymes. pPro24-gfp contains GFP but all others are empty. pdCas9-bacteria ...20341 mEos2 (fluorescent protein) Loren Looger Photoactivable Fluorescent Protein Expression: contains mEos2...mEos2 with an N-terminal His tag. Photoactivable fluorescent proteins like this one can be used in super...fluorophore-tagged MS2 (such as that found in pZS*12-MS2-GFP ) to track RNA localization of your gene of interest...lactose/IPTG inducible) Flag, His, Strep, mOCR, MBP, GFP, with and without SUMO or TEV cleavage sites to remove...Gram-negative bacteria. pAK Series Various Plasmids pTac GFP, Luciferase (LuxCDABE) Attila Karsi Broad host range...

  7. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ... of GFP, it's variants, and their relevant mutations, check out Marcy's post from last week on GFP. Additionally... a few hours. For example, superfolder GFP (sfGFP) and mNeonGFP can fold in <10min at 37°C, mCherry takes...fluorescence. Examples for such photoactivatable or photoconvertible proteins are PA-GFP, Dendra2, and the mEOS...affected by the temperature. For instance, enhanced GFP (EGFP) was optimized for 37°C, and is therefore most... the discovery of the Green Fluorescent Protein (GFP), a protein from the jellyfish Aequorea Victoria....below (or emission wavelenght range).  By mutating GFP, the variants blue FP (BFP), cyan FP (CFP), and yellow...be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be split into two halves, which are non-fluorescent...

  8. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...both the GFP and the RFP proteins in the nucleus. During the first round of translation, the GFP proteins...Compartment mCherry-Rab5 BFP-Rab5 GFP-Rab5B Vacuolar & Budding Compartment GFP-Rab4B Mitochondria mito-BFP...GTPase in division) GFP-Mff (outer membrane protein) Late Endosome mCherry-Rab7A GFP-Rab7A     ...same cell; by photoswitching PSmOrange and photoactivating PAGFP in separate subpopulations of mitochondria...protein Knock-off, is made up of three components: a GFP protein that binds to the coding region of a reporter...reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) a RFP protein that binds to the 3’ UTR of the reporter...these dCas9s with one of three fluorescent proteins (GFP, BFP and mCherry), the authors were able to visualize...

  9. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post
    ...Since the discovery of GFP over 50 years ago, the growing spectrum of fluorescent proteins (FPs) has ...repair pathway, a new user-defined DNA sequence (like GFP) can be inserted at the DSB. CRISPR has allowed scientists...toolkit offers C- and N-terminal tagging vectors (eg GFP, Flag, YFP, Strep, TEV-V5) with either blasticidin...NanoLuc), fluorescent proteins (TagGFP2, TagBFP, TagRFP, and T2A-TurboGFP-PEST), and small epitope tags ...library containing several types of fluorescent (EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP...about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins Use Rosella to Monitor...

  11. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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    Blog Post
    ...from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection.  ...sun, from the standard oldies but goodies (e.g., EGFP and YFP) to the new and improved fruit colors (e.g...(e.g., apple, papaya, and tomato) and the photoactivatable fluors (e.g. Phamret and Dendra). It’s just...

  12. Fluorescent Protein Guide: Empty Backbones

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    Collection
    ...Expression pRSETA-PAGFP - Bacterial Expression tandem PA-GFP-KanR - Yeast Expression pFA6a-PA-GFP-kanMX6 - Yeast...Expression pFA6a-link-yEPAGFP-CaUra3 - Yeast Expression mPA-GFP-N1 - Mammalian Expression mPA-GFP-C1 - Mammalian...then scientists have engineered numerous GFP-variants and non-GFP proteins that result in a diverse set ...tagging with mCherry, mOrange, mCerulean pET GFP - C-terminal GFP for bacterial expression Davidson Lab Plasmids...Expression EGFP 488 507 34 6 Prone to dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian...Mammalian Expression PA-GFP 504 517 14 Monomer (A206K) pPAGFP-N1 - Mammalian Expression pPAGFP-C1 - Mammalian ...with mCherry, Cerulean, Citrine, and eGFP pPD95_75 - C-terminal GFP for C. elegans expression pHT101-mCherry...

  13. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...fluorescent protein that could be co-imaged with GFP using the same wavelength for excitation. The result...and colleagues (8) were interested in using photoactivatable, optogenetic accuators to depolarize or hyperpolarize...
Showing: 1 - 13 of 13 results