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We narrowed to 14 results for: plko

Showing: 1 - 14 of 14 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more slowly than standard plasmids. Find pLKO.1 - TRC... TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary elements for packaging, ...RNAi experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David...target gene knockdown. Note: this plasmid was called pLKO-Tet-On in the original publication but its name ...manual provided by the Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the...

  3. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...Recently I learned that Addgene’s pLKO.1 cloning protocol is viewed around 3,000 times each month. I ...protocol on the Addgene website in December 2006, the pLKO.1-TRC cloning vector (deposited by David Root of...How would the thousands of other scientists using pLKO.1 variants learn about those updates? This is a ...

  6. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... Protocols pLKO.1 Protocol pLKO.1 - TRC Cloning Vector Addgene Plasmid 10878...Table of Contents A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids....2 Order oligos compatible with pLKO.1 C. Cloning shRNA oligos into pLKO.1 C.1 Recommended materials C.2...Sequence of pLKO.1 TRC-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-TRC Cloning...arising from the use of this vector. A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector...marker encoded in pLKO.1 allows for convenient stable selection. Figure 1 : Map of pLKO.1 containing an ...conjunction with the pLKO.1 TRC-cloning vector. Addgene Plasmid Description pLKO.1 – TRC control Negative...

  7. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...vector for mammalian genome editing Tet-pLKO-puro and Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression...Mammalian Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning... Backbones Neomycin (G418) Mammalian, Varies Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression ...PGK driven puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral shRNA expression lentiCRISPRv2...vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd gen lentiviral backbone for cloning... for gene expression Thy1.1 Mammalian pLKO.3 Thy1.1 - Lentiviral shRNA expression Gentamicin...

  8. Lentivirus Plasmids

    Type
    Collection
    ...lenti". ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puro...plasmid 24150 for hygro resistance. Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty...; includes a stuffer for easy cloning Root 14748 pLKO.3G 3rd U6-driven shRNA empty plasmid with EGFP marker...for Thy1.1 selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin...plasmid 21374 for dtomato version. Welm 21916 Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin ...

  10. Tetracycline Inducible Expression

    Type
    Collection
    ...Description Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for inducible expression...selection. TetR H1-2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning... selection. TetR H1-2O2 Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible...

  11. Mammalian RNAi Tools

    Type
    Collection
    ...Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol Other Addgene viral protocols...

  12. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Lentiviral BsmBI yes, cut S. pyogenes EGFP Ebert pLKO.1-puro U6 sgRNA BfuAI stuffer 50920 Mammalian/Lentiviral...Mammalian see plasmid page yes, cut S. pyogenes BFP Kuhn pLKO.1-puro U6 sgRNA BfuAI large stuffer 52628 Mammalian...Mammalian/Lentiviral BsmBI none S. pyogenes ccdB Wei pLKO5.sgRNA.EFS.PAC 57825 Mammalian/Lentiviral BsmBI none...60226 Mammalian/AAV SapI none S. pyogenes Zhang pLKO5.sgRNA.EFS.GFP 57822 Mammalian/Lentiviral BsmBI none...Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe pLKO5.sgRNA.EFS.tRFP657 57824 Mammalian/Lentiviral BsmBI...Lentiviral BsmBI yes, cut S. pyogenes EGFP Ebert pLKO5.sgRNA.EFS.tRFP 57823 Mammalian/Lentiviral BsmBI ...

  13. Lentiviral Vector Guide

    Type
    Guide
    ... More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols Lentiviral Vector...

  14. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... Leong Ho 102970 pLKO.1P shFXN_1 FXN Friedreich ataxia Richard Possemato 102971 pLKO.1P shFXN_2 FXN Friedreich...Friedreich ataxia Richard Possemato 102975 pLKO.1P shSOD1 SOD1 ALS Richard Possemato 103913 HTRA2_PDZ_... HA-KIF5A KIF5A HA CMV ALS Rosalind Segal 167294 pLKO.1 puro_shTBK1_3185 TBK1 ALS Agnel Sfeir 169375 pET3d-SF-DCTN1...
Showing: 1 - 14 of 14 results