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We narrowed to 10 results for: plko 1 lentiviral shrna vector

Showing: 1 - 10 of 10 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab...Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more slowly than standard plasmids. Find pLKO.1 - TRC...scramble shRNA - This plasmid from David Sabatini’s lab is a 3rd generation lentiviral vector that contains...TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary elements for packaging, reverse...It can be used with 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such ... lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the Zhang lab is used to deliver...contains a scrambled shRNA. This scrambled shRNA is used as a negative control for mammalian RNAi experiments...

  2. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ...backbones for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -...Fire's set of vectors for C. elegans research was our most requested item of 2005. shRNA - In 2006, we...you're familiar with the pLKO plasmids you'll note that those are lentiviral constructs. In 2007 we continued...continued to see requests grow for lentiviral packaging and expression vectors, including pMD2.G and psPAX2....beta-catenin reporter M50 Super 8x TOPFlash, scramble shRNA for use as a negative control, and empty backbones...-puro for retroviral expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs ...

  3. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ... compound of interest. Lentiviral vectors Popular Lentiviral Transfer Vectors    Expressing your gene...generation lentiviral vector backbone from the Shih lab. You can find more lentiviral expression vectors (empty...building block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial...experiments. Gamma-retroviral vs. lentiviral vector systems Gamma-retroviral vectors have a relatively high transduction...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning... Viral vector topic page Beginners guide to viral vectors Quick guide to all things lentivirus Resources...Murine Stem Cell Virus (MSCV) genomes whereas lentiviral vectors are derived from the human immunodeficiency...

  4. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...use of this vector. A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the...Back to Top A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium The pLKO.1 cloning vector is the backbone...digests. Digest pLKO.1 TRC-cloning vector with AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep...Table of Contents A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids...Order oligos compatible with pLKO.1 C. Cloning shRNA oligos into pLKO.1 C.1 Recommended materials C.2 Annealing...encoded in pLKO.1 allows for convenient stable selection. Figure 1 : Map of pLKO.1 containing an shRNA insert...Appendix I.1. Sequence of pLKO.1 TRC-Cloning Vector Click here to see the sequence of pLKO.1 TRC-cloning...

  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...cloning vector - Lentiviral shRNA expression pLJM1-EGFP - 3rd gen lentiviral vector for EGFP fusion; PGK driven...Mammalian Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning...pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd gen... v2 - 3rd gen lentiviral vector for mammalian genome editing Tet-pLKO-puro and Tet-pLKO-neo - Tet-inducible...puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...expression vector with N- or C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF...

  6. Lentivirus Plasmids

    Type
    Collection
    ...expression of shRNAs; addition of Cre turns on shRNA expression. See article for more cre-lox shRNA expression...Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puro resistance....resistance. Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid; includes a ...Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...Reiser Return to Top Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material...

  7. Mammalian RNAi Tools

    Type
    Collection
    ...Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more....delivery of a plasmid. shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred method...Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol...BioRender.com. shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids...information on shRNA design and delivery, see resources below. Figure 1: Overview of shRNA-mediated RNA ...protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design...the viral vector is taken up by a host, the viral genome is transcribed, producing the shRNA. Dicer, an...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ...Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection...85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design of inducible shRNA expression... pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate shRNA efficacy by fluorescence, use Plasmid...pLVUT-tTR-KRAB Lentiviral Tet-On vector for inducible expression of transgene and/or shRNA. See article ...PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral Tet-On vector with blasticidin selection. See Plasmid...Alexeyev 17492 pLenti CMV TetR Blast (716-1) Lentiviral Tet-On vector expressing TetR from CMV promoter TetR...plasmid. A single vector approach may not have as much flexibility and control as a dual vector design, but ...

  9. Lentiviral Vector Guide

    Type
    Guide
    ...Viral Vectors Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations...More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols Lentiviral Vector...Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses typically ranges from... Things Lentivirus Lentiviral Vector Uses and Overview Overcoming the Challenges of Lentiviral Production...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...Second-generation lentiviral plasmids greatly improved the safety of lentiviral vector production by removing...is necessary to properly utilize lentiviral vectors. Some lentiviral plasmids (mostly third-generation...

  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... EF1-alpha ALS George Church 222870 pLKO.1 mTagBFP2 rat DJ-1 shRNA PARK7 U6 Parkinson's Timothy Ryan 223563...pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #1) NR4A2 GFP CMV Parkinson's Glenn Schafe 233274 pAAV-CMV-GFP-pA U6 shRNA (Rat ...Fawzi 127194 RP1B FUS 1-163 QQ4xSS #1 FUS His T7 ALS Nicolas Fawzi 127195 RP1B FUS 1-163 QQ4xSS #2 FUS His... type 1 scFv [L24/1] ITPR1 CMV Spinocerebellar ataxia James Trimmer 206790 IP3 receptor, type 1 scFv [...Parkinson's David Root 78523 pFUChW Parkin shRNA Mul1 shRNA PRKN H1 Parkinson's David Chan 78622 pX335... Leong Ho 102970 pLKO.1P shFXN_1 FXN Friedreich ataxia Richard Possemato 102971 pLKO.1P shFXN_2 FXN Friedreich...237005 VCP-HaloTag Vector VCP Halo CMV ALS Promega Corporation 237006 HaloTag-VCP Vector VCP Halo CMV ALS...
Showing: 1 - 10 of 10 results