We narrowed to 9 results for: plko 1 puro shrna vector

...standard plasmids. Find pLKO.1 - TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary...experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab...provided by the Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the S. pyogenes...and expressing new shRNA sequences. For more information, see the Addgene’s pLKO.1 protocol. Pro-tip: ...pSpCas9(BB)-2A-Puro (PX459) V2.0 - This plasmid from the Zhang lab is a mammalian vector that expresses...scramble shRNA - This plasmid from David Sabatini’s lab is a 3rd generation lentiviral vector that contains...contains a scrambled shRNA. This scrambled shRNA is used as a negative control for mammalian RNAi experiments...

...empty backbones for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids...Fire's set of vectors for C. elegans research was our most requested item of 2005. shRNA - In 2006, we...TOPFlash, scramble shRNA for use as a negative control, and empty backbones such as pBABE-puro for retroviral...Plasmids -  If you're familiar with the pLKO plasmids you'll note that those are lentiviral constructs. ...requests grow for lentiviral packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two...

...Lentiviral vectors Popular Lentiviral Transfer Vectors    Expressing your gene of interest pLenti-puro (Plasmid...building block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning... vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This is a lentiviral...research purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating viral...and expression of these vectors when used in experiments. Retroviral vectors make up a large toolbox ...Additionally, retroviral vectors can regulate gene expression by delivering shRNA or miRNA. The ever expanding...

... Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puromycin selection. See plasmid...Diane Mathis 21915 Tet-pLKO-puro 3rd Inducible expression of shRNA with puromycin selection. See plasmid...selection. Bob Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid. Includes a ...engineered into viral vectors for safe use in the lab. One safety feature of lentiviral vectors is that the components...stuffer for easy cloning. David Root 14748 pLKO.3G 3rd U6-driven shRNA empty plasmid with EGFP marker. See plasmid...(Cre-lox), stable expression of shRNAs; addition of Cre turns on shRNA expression. See Ventura et al. ...the gene of interest. Can also express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in...

...Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression pLJM1-EGFP... 3rd gen lentiviral vector for mammalian genome editing Tet-pLKO-puro and Tet-pLKO-neo - Tet-inducible...selection Puromycin Mammalian pcDNA3.1-(-)-Pur - Eukaryotic expression vector Tet-pLKO-puro - ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...basal activity shRNA/RNAi Gene silencing Browse RNAi empty vectors for cloning in shRNAs, including plasmids...lentiviral vector for EGFP fusion; PGK driven puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral...destination vector for gene expression Thy1.1 Mammalian pLKO.3 Thy1.1 - Lentiviral shRNA expression...

...Back to Top A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium The pLKO.1 cloning vector is the backbone...use of this vector. A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the... Map of pLKO.1 containing an shRNA insert. The original pLKO.1-TRC cloning vector has a 1.9kb stuffer ...digests. Digest pLKO.1 TRC-cloning vector with AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep...Table of Contents A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids...Order oligos compatible with pLKO.1 C. Cloning shRNA oligos into pLKO.1 C.1 Recommended materials C.2 Annealing...Appendix I.1. Sequence of pLKO.1 TRC-Cloning Vector Click here to see the sequence of pLKO.1 TRC-cloning...

...21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection...85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design of inducible shRNA expression... pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate shRNA efficacy by fluorescence, use Plasmid...plasmid. A single vector approach may not have as much flexibility and control as a dual vector design, but ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or search...selection. TetR H1-2O2 Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression...TRE3GS Xiaojun Lian 171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On...

... EF1-alpha ALS George Church 222870 pLKO.1 mTagBFP2 rat DJ-1 shRNA PARK7 U6 Parkinson's Timothy Ryan 223563...pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #1) NR4A2 GFP CMV Parkinson's Glenn Schafe 233274 pAAV-CMV-GFP-pA U6 shRNA (Rat ...Fawzi 127194 RP1B FUS 1-163 QQ4xSS #1 FUS His T7 ALS Nicolas Fawzi 127195 RP1B FUS 1-163 QQ4xSS #2 FUS His... type 1 scFv [L24/1] ITPR1 CMV Spinocerebellar ataxia James Trimmer 206790 IP3 receptor, type 1 scFv [...Parkinson's David Root 78523 pFUChW Parkin shRNA Mul1 shRNA PRKN H1 Parkinson's David Chan 78622 pX335... Leong Ho 102970 pLKO.1P shFXN_1 FXN Friedreich ataxia Richard Possemato 102971 pLKO.1P shFXN_2 FXN Friedreich...115182 pLD-puro-Cc-PARK7WT-VA PARK7 His, Flag, Streptactin CMV Parkinson's Mohan Babu 115183 pLD-puro-Cc-PARK7V51G-VA...

...Viral Vectors 101 topics More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols...Guides Viral Vectors Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral...lentiviral vectors that can be safely used in laboratory settings. These engineered lentiviral vectors have ...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...gamma-retroviruses, see Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses typically...plasmids. Lentiviral Vector Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the..., see Addgene’s viral vector protocols . Figure 5: Overview of lentiviral vector production. Created with...