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We narrowed to 11 results for: plko.1-puro

Showing: 1 - 11 of 11 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary elements for packaging, reverse...Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more slowly than standard plasmids. Find pLKO.1 - TRC...provided by the Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the S. pyogenes.... Find lentiGuide-Puro. pSpCas9n(BB)-2A-GFP (PX461) - Similar to pSpCas9(BB)-2A-Puro (PX458), pSpCas9n...best to go with pSpCas9(BB)-2A-Puro (PX459) V2.0. Find pSpCas9(BB)-2A-Puro (PX459). Thank you! We would... experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David...in the puromycin cassette that makes it less efficient in some cell lines. pSpCas9(BB)-2A-Puro (PX459)...

  2. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ...backbones for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids...Plasmids -  If you're familiar with the pLKO plasmids you'll note that those are lentiviral constructs. In...negative control, and empty backbones such as pBABE-puro for retroviral expression and pLVTHM, a lentiviral...

  3. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...protocol on how to use the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid...GFP expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is an RNA-mediated...Vectors    Expressing your gene of interest pLenti-puro (Plasmid #39481) You can clone your gene of interest...here. Cloning and expressing new shRNA sequences pLKO (Plasmid #10878) This plasmid has been used by ...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning...Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus...

  4. Lentivirus Plasmids

    Type
    Collection
    ...Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puro resistance. See plasmid...Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin selection. See plasmid ...variations. Campeau 39481 pLenti-puro 3rd CMV driven expression of cDNA. Puro selection. Shih 25737 pSLIK-...21916 Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin selection. See plasmid 21915 for Puro resistance...plasmid 24150 for hygro resistance. Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid...; includes a stuffer for easy cloning Root 14748 pLKO.3G 3rd U6-driven shRNA empty plasmid with EGFP marker...CMV-driven EGFP fusion; can be used for cDNA expression; puro resistance Sabatini 14883 FUGW 3rd hUbC-driven EGFP...

  5. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...vectors (e.g. pBV-Luc) and gene silencing (e.g. pLKO.1 - TRC cloning vector). Check out our complete guide...and carry the metallothionein promoter (e.g. pMT-puro). Worm vectors There are several approaches to studying...

  6. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...vector for mammalian genome editing Tet-pLKO-puro and Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression... plasmids with Neomycin selection Puromycin Mammalian Tet-pLKO-puro - Tet-inducible lentiviral...for EGFP fusion; PGK driven puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral shRNA expression...lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression pLJM1-EGFP - 3rd ... lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd gen lentiviral backbone for cloning...AdEasier®-1 cells (strain) - Bacterial strain that contains AdEasy®-1 plasmid...Ligation Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression plasmid for cloning...

  7. Tetracycline Inducible Expression

    Type
    Collection
    ...21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection... TetR H1-2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and...selection. TetR H1-2O2 Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression...Tet-On 3G rtTA TRE3GS Xiaojun Lian 171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression...for CRISPRa Emma Rawlins 72835 pMK243 (Tet-OsTIR1-PURO) Expresses OsTIR1 under the control of a TRE3GS ...and releases tet O, enabling transcription (Figure 1). While TetR and tet O could be a basic tool to control...skip ahead to view highlighted Tet plasmids . Figure 1: Tet-regulated expression systems. Left: natural TetR...

  8. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Ebert pLKO.1-puro U6 sgRNA BfuAI stuffer 50920 Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe ...)-2A-Puro (PX462) V2.0 62987 Mammalian BbsI yes, nick S. pyogenes Puro Zhang pSpCas9(BB)-2A-Puro (PX459...pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI none S. pyogenes Puro Zhang ...see plasmid page yes, cut S. pyogenes BFP Kuhn pLKO.1-puro U6 sgRNA BfuAI large stuffer 52628 Mammalian/...pyogenes Puro Moffat lenti sgRNA(MS2)_puro backbone 73795 Mammalian/Lentiviral U6 none S. pyogenes Puro Zhang...pyogenes Puro Ebert pL-CRISPR.SFFV.PAC 57829 Mammalian/Lentiviral BsmBI yes, cut S. pyogenes Puro Ebert ...pLenti SpBsmBI sgRNA Puro 62207 Mammalian/Lentiviral BsmBI none S. pyogenes Puro Maehr pKLV-U6gRNA-EF(...

  9. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Table of Contents A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids...Order oligos compatible with pLKO.1 C. Cloning shRNA oligos into pLKO.1 C.1 Recommended materials C.2 Annealing...information Back to Top A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium The pLKO.1 cloning vector is the...Appendix I.1. Sequence of pLKO.1 TRC-Cloning Vector Click here to see the sequence of pLKO.1 TRC-cloning... Protocols pLKO.1 Protocol pLKO.1 - TRC Cloning Vector Addgene Plasmid 10878...arising from the use of this vector. A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector... Map of pLKO.1 containing an shRNA insert. The original pLKO.1-TRC cloning vector has a 1.9kb stuffer ...

  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...SOD1 EF1-alpha ALS George Church 222870 pLKO.1 mTagBFP2 rat DJ-1 shRNA PARK7 U6 Parkinson's Timothy Ryan...Fawzi 127194 RP1B FUS 1-163 QQ4xSS #1 FUS His T7 ALS Nicolas Fawzi 127195 RP1B FUS 1-163 QQ4xSS #2 FUS His... type 1 scFv [L24/1] ITPR1 CMV Spinocerebellar ataxia James Trimmer 206790 IP3 receptor, type 1 scFv [... Leong Ho 102970 pLKO.1P shFXN_1 FXN Friedreich ataxia Richard Possemato 102971 pLKO.1P shFXN_2 FXN Friedreich...115182 pLD-puro-Cc-PARK7WT-VA PARK7 His, Flag, Streptactin CMV Parkinson's Mohan Babu 115183 pLD-puro-Cc-PARK7V51G-VA... pLD-puro-Cc-PARK7C53A-VA PARK7 His, Flag, Streptactin CMV Parkinson's Mohan Babu 115185 pLD-puro-Cc-PARK7H126A-VA...pLD-puro-Cc-PARK7E163K-VA PARK7 His, Flag, Streptactin CMV Parkinson's Mohan Babu 115187 pLD-puro-Cc-CR-PARK7WT-VA...

  11. Lentiviral Vector Guide

    Type
    Guide
    ...More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols Lentiviral Vector...typically ranges from 8–10 kb, encoded on RNA (Figure 1). This RNA is reverse transcribed into the provirus...been removed or mutated for increased safety. Figure 1: Wild-type lentivirus genome. Created with BioRender.com...genes (for example, vif , vpr , vpu , and nef for HIV-1). The LTRs flank all of these genes, and anything ...transgene and LTRs (chimeric 5’ LTR) Packaging plasmid 1 — contains gag and pol Packaging plasmid 2 — contains...and third-generation systems, please refer to Table 1. Figure 4: Third-generation lentiviral plasmids. Created...heterologous enhancer/promoter such as CMV or RSV Table 1. Summary of key differences between second- and third-generation...
Showing: 1 - 11 of 11 results