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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 11 results for: pspax2

Showing: 1 - 11 of 11 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...The top 15 plasmids requested from Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral packaging plasmid...most requested plasmid of all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2...

  3. Lentivirus Plasmids

    Type
    Collection
    ...with additional packaging components Trono 63586 psPAX2-D64V 2nd Packaging For making Integrase deficient...plasmids along with a plasmid encoding Rev Trono 12260 psPAX2 2nd Packaging Contains Gag, Pol, Rev, and Tat; ...

  5. CRISPR References and Information

    Type
    Collection
    ...cloning lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF, 2.4 MB Zhang ...and lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Pooled libraries are...

  6. Viral Production at Addgene

    Type
    Blog Post
    ...produced in 293T cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono...

  8. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... HEK293T cells with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260). HeLa cells were infected with...

  9. Viral Production

    Type
    Collection
    ...produced in 293T cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono...

  10. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid for producing viral particles...Recommended Materials Material Vendor and catalog # psPAX2 Addgene #12260 pMD2.G Addgene #12259 HEK-293T cells... transfection: 1 μg pLKO.1 shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid...

  11. Lentivirus Production

    Type
    Protocol
    ...Reagent Amount per 10 cm dish* Packaging Plasmid ( psPAX2 ) 1.3 pmol Envelope Plasmid ( pMD2.G ) 0.72 pmol...
Showing: 1 - 11 of 11 results