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We narrowed to 4 results for: rfp gfp lc3 plasmid

Showing: 1 - 4 of 4 results
  1. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...in red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe that releases...molecule of GFP-tagged LC3 for every one molecule of RFP-tagged LC3∆G. Like the unmodified LC3, GFP-LC3 is conjugated...autophagy biosensor is the GFP-LC3-RFP-LC3ΔG probe. It’s similar to the mRFP-GFP-LC3 Tandem Fluorescent Protein...is estimated by the GFP/RFP ratio: a decrease in GFP fluorescence relative to RFP fluorescence suggests... in autophagic flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky, Daniel J.,...Addgene! mRFP-GFP-LC3 tandem fluorescent protein This autophagy biosensor contains mRFP and GFP fused to the...chain 3B, or LC3. LC3 is a commonly used marker for measuring autophagic flux. mRFP-GFP-LC3 functions as...
  2. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...microscopy analysis is time consuming. GFP-tagged Atg8p or LC3: ATG8 is part of a group of genes that ...autophagosomes and autophagic bodies in yeast. A GFP-tagged version of LC3, the mammalian Atg8p homolog, can also... a relatively pH-insensitive RFP variant, DsRed.T3 and a pH-sensitive GFP variant, super ecliptic pHluorin...with autophagosomal membranes, so tagging it with GFP allows for tracking the localization or accumulation...Fluorescent Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy...autophagy-independent manner. Additionally, tracking Atg8p or LC3 doesn’t provide information about the contents of...in the vacuole or lysosome, since both Atg8p and LC3 are degraded or released once an autophagosome fuses...
  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... Gerlich 25999 LV-GFP Chromatin H2B GFP Elaine Fuchs 11680 H2B-GFP Chromatin H2B GFP Geoff Wahl 21217 ...Voeltz 118084 pLenti-EB1-EGFP Microtubules EB1 EGFP Ken-Ichi Takemaru 122871 GFP-hCCDC11 Centriolar satellites...pIRESneo-EGFP-alpha Tubulin Microtubules alpha-Tubulin EGFP Patricia Wadsworth 27123 eTC GFP beta-actin...beta-Catenin EGFP Alpha Yap 67937 mouse E-cadherin GFP (423) Adherens junctions E-cadherin EGFP Alpha Yap...38277 pMXs-puro GFP-p62 Autophagosomes Sequestosome-1 EGFP Noboru Mizushima 38272 pMXs-IP GFP-WIPI-1 Autophagosomes...Yoshimori 110060 FUW mCherry-GFP-LC3 Autophagosomes LC3 mCherry, EGFP Anne Brunet 61204 pCMU-AUTr Autophagosomes...61803 GFP-Rab7A Endosomes (late) Rab7a AcGFP Gia Voeltz 12605 GFP-rab7 WT Endosomes (late) RAB7 GFP Richard...
  4. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ...autophagic flux by pH-sensitive fluorescence (pMRX-IP-GFP-LC3-RFP) An Autophagic Flux Probe that Releases an Internal...troponin C Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin Protein) for imaging...FLAMP1 and FLAMP2 Chu Lab FLAMP Plasmids Jun Chu cGMP (cyclic GMP) FlincG GFP-based cGMP sensor Differential... Optogenetics Plasmids Viral Service: AAV Biosensors Luciferase Plasmids Promega Plasmid Collection Explore...2020 GENIE Project Calcium jGCaMP7 High-performance GFP-based calcium indicators (Constitutive or Cre-dependent...imaging of Ca 2+ dynamics in endoplasmic reticulum GFP-Aequorin Protein Sensor for Ex Vivo and In Vivo Imaging...
Showing: 1 - 4 of 4 results