...decrease in red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe that...molecule of GFP-tagged LC3 for every one molecule of RFP-tagged LC3∆G. Like the unmodified LC3, GFP-LC3 is conjugated...autophagy biosensor is the GFP-LC3-RFP-LC3ΔG probe. It’s similar to the mRFP-GFP-LC3 Tandem Fluorescent Protein... in autophagic flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky, Daniel J.,...chain 3B, or LC3. LC3 is a commonly used marker for measuring autophagic flux. mRFP-GFP-LC3 functions as...is estimated by the GFP/RFP ratio: a decrease in GFP fluorescence relative to RFP fluorescence suggests...Addgene! mRFP-GFP-LC3 tandem fluorescent protein This autophagy biosensor contains mRFP and GFP fused to...

...microscopy analysis is time consuming. GFP-tagged Atg8p or LC3: ATG8 is part of a group of genes that ...autophagosomes and autophagic bodies in yeast. A GFP-tagged version of LC3, the mammalian Atg8p homolog, can also... a relatively pH-insensitive RFP variant, DsRed.T3 and a pH-sensitive GFP variant, super ecliptic pHluorin...with autophagosomal membranes, so tagging it with GFP allows for tracking the localization or accumulation...autophagy-independent manner. Additionally, tracking Atg8p or LC3 doesn’t provide information about the contents of...in the vacuole or lysosome, since both Atg8p and LC3 are degraded or released once an autophagosome fuses...Fluorescent Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy...

... Gerlich 25999 LV-GFP Chromatin H2B GFP Elaine Fuchs 11680 H2B-GFP Chromatin H2B GFP Geoff Wahl 21210 ...Autophagosome LC3 EGFP Tamotsu Yoshimori 24920 pEGFP-LC3 (human) Autophagosome LC3 EGFP Toren Finkel 14435...Rubinsztein 11546 EGFP-LC3 Autophagosome LC3 EGFP Karla Kirkegaard 61205 pCMB-AUTr Autophagosome LC3(ATG8) mCherry...Paxillin-pEGFP Focal Adhesions paxillin GFP Rick Horwitz 26720 RFP-zyxin Focal Adhesions Zyxin RFP Anna Huttenlocher...Autophagosome LC3 mCherry, GFP Anne Brunet 40827 mCherry-hLC3B-pcDNA3.1 Autophagosome LC3 mCherry David Rubinsztein...61803 GFP-Rab7A Late endosomes Rab7a AcGFP Gia Voeltz 12605 GFP-rab7 WT Late endosomes RAB7 GFP Richard...components. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI 110060 FUW mCherry-GFP-LC3 Autophagosome...

...autophagic flux by pH-sensitive fluorescence (pMRX-IP-GFP-LC3-RFP) An Autophagic Flux Probe that Releases an Internal... Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service: AAV Biosensors...troponin C Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin Protein) for imaging...FLAMP1 and FLAMP2 Chu Lab FLAMP Plasmids Jun Chu cGMP (cyclic GMP) FlincG GFP-based cGMP sensor Differential...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Autophagy Autophagosome maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation process...

...Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid Collection You May Also...full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human... Addgene ID Plasmid Allen Institute ID Tag Protein Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin Matrix...Autophagy-related protein LC3 B Autophagosomes 101784 SLC25A17-mEGFP AICSDP-27 mEGFP Peroxisomal membrane protein...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science...induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited...