15 Hot Plasmids from 2017
Type
Blog Post
...NGG)
Editing window ~2 nt
85175
pBK-EE-BE3
SpCas9 (NGG)
Editing window ~2 nt
85176
pBK-YE2-...simultaneously measuring the activities of CaMKII and RhoA during induction of structural plasticity in single...expression plasmids, FRET constructs, and CaMKII and RhoA sensors.
Laviv, et al. Nat Methods. 2016. PubMed...quantum yield of 0.54) and mScarlet-H with M164H (2-fold improvement in photostability compared to mScarlet...SpCas9 base editors with editing windows as small as 1-2 nucleotides!
To reduce off-target effects associated...pBK-YE2-BE3
SpCas9 (NGG)
Editing window ~2 nt
85177
pBK-YEE-BE3
SpCas9 (NGG)
Lower on-target activity...activity than BE3; editing window ~1-2 nt
87438
pET42b-HF-BE3
HF-Cas9 (NGG)
Very low off-target activity...