...is a new software tool used to design gRNAs for custom CRISPR screens targeting a (long or short) list...Glance!  CRISPR knockout screens: Full genome vs. custom screens Full genome CRISPR knockout screens have...hypothesis driven research question. Examples of custom CRISPR screens Differentially expressed genes  ...massive amount of descriptive transcriptomics data. Custom CRISPR screens designed to target differentially...microbe or a cytokine is added to a cell). With a custom CRISPR screen and the appropriate phenotypic readout...surface proteins. One of the first challenges with a custom screen approach is to identify an appropriate list...the Output_Short file directly. We have used CustomArray, Inc for this purpose.  The gRNA oligos are...

...Addgene’s customer service philosophy Customer service can be a hassle. You finally make it through a...of our core values here is to provide unmatched customer support, as this is vital to ensuring that our...technical, or quality control issues. Improving customer support with self service We strive to make our...waiting for an answer. Luckily, there’s a trend in customer support to address this exact problem, and it’... customer support...

...posted sequence in the "View Sequence" link. Is your custom primer found within the Addgene sequencing results...require additional sequencing and need to design a custom primer, Addgene recommends using Addgene's sequencing...

... as follows: In the Chart Editor , select the Customize tab. Select the Series drop down menu. Select ...trendline in Google Sheets as follows: In the Customize tab, select the Label drop down menu and choose...

...Having spent that long in customer service, I've daydreamed about the ideal customer service interaction -...to any biology-related customer support interaction. Before contacting customer service In an age of Tweeting...answered hundreds of phone calls and thousands of customer service emails in my six years as a senior scientist... - one that gives our customers the most utility in the least amount of time. Though I now spend far less...my years of accumulated wisdom so that you, the customer, can get the most out of your email or phone call...section are really, truly frequently asked by customers (and I expect this is the case at most companies...are some pointers to expedite and improve your customer service experience: Make sure you’re contacting...

...support through the customer’s perspective is good. If you’ve never participated in a customer support conversation...the types of questions customers ask, and there are also differences in customers’ personalities and their...reviewing customer data sets, troubleshooting protocols, analyzing information, and solving customer problems... product issue in the lab. The issue was with a custom-made reagent to manipulate cells (manufactured ...multiple languages - that makes communication with customers from around the world easier. You should also ...expectations. If you are willing to listen to a customer’s concerns and are patient enough to find the real...are the most popular ways to communicate with customers. Online live chat is a convenient alternative ...

... it out. 4:00 PM--Respond to customer help tickets. Catch up on customer help tickets, and respond when...advocate for the customer and help build software and technology tools with the customer in mind. You can...Oxford Nanopore Technologies, I work with our customers in the US to learn where the gaps are in their... so many different people! I communicate with customers, software developers, as well as team members ... It’s divided into the key takeaway themes and customer pain points we’ve identified. I can’t wait to ...know what to work on next to help alleviate any customer pain points. Many thanks to our guest blogger...

...usual quality and success. Customer and technical support Our customer and technical service brands...helpful and the customer and technical service teams put 100% effort into helping our customers every day in...scientific fields.” Thanks to our legal team, and great customer service, somehow we have scientists actually loving...offer online for Addgenies and to serve external customers. I also can’t wait to see where our new data initiatives...

...applications in plants. This smaller, easily customizable vector functions in multiple species, including...for many applications, the plasmid can also be customized due to its modular structure. Two multiple cloning... regulatory elements to be inserted, further customizing gene expression. Sahoo et al. have tested the...Western blotting. Given its versatility and customizability, pSiM24 can be used not only for plant engineering...

...nuclease-deficient Cas9 (dCas9) protein and a customized s ingle g uide RNA (sgRNA) designed with a 20...with ampicillin resistance, wherein the N20 can be custom designed to target arbitrary sequences in the genome...controlled sgRNA cassette, wherein the GN19 can be custom designed to target sequences in the genome. The...fusion expression plasmid 44248 pgRNA-humanized A customizable gRNA expression plasmid for use with either ...editing bacterial genomes 44251 pgRNA-bacteria A customizable gRNA expression plasmid for use with the bacterial...

...primary role is customer support, but there are eight other teams who still retain a customer support element...other responsibilities. Knowing which aspects of customer support each team “owns” is critical for day-to-day...-to-day operations and keeping our customers happy, but inevitably there will be gray areas that either...

... spring of 2011, the groups describe a set of customized plasmids that can be used with the recently developed...plasmids, which are available as a kit from Addgene , custom arrays consisting of 12-31 repeats can be assembled...here . References Efficient design and assembly of custom TALEN and other TAL effector-based constructs for...697-8. Pubmed PMID: 21822241 . TAL effectors: customizable proteins for DNA targeting Bogdanove AJ, Voytas...

.... We are the Customer Support Specialists here at Addgene. If you’ve called our customer service line ...really getting at one bigger question: How does a customer place an Addgene order using a PO number? Why ...

...Toolkit for constructing fusion proteins with customizable N- and C-terminal modules   Thanks to the lab...Technologies. The MultiSite Gateway Kit is a customizable Gateway®-based toolkit that allows scientists...: 10.1126/science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector...LacZ, puromycin or CreERT2 can be combined with custom-cloned vectors in a Multisite Gateway reaction ...imaging and flow cytometry applications Designing customized fluorescent reporters can be challenging enough...ChIP, and more - all without the need to develop custom antibodies. Unfortunately, while there are many...

...sequences from a shared database of custom common features. Second, customers have asked for a way to annotate... involves iterative refinements in response to customer feedback. An example of this approach is SnapGene...

...assembled a cross-team team, with members from the Customer Service, Data Curation, Outreach, Product, Scientist...particular and our deep understanding of Addgene’s customer’s needs. Categories We created a categorization...of our information in order to more granularly customize the search engine, and a scoping mechanism that...

...post was contributed by Oskar Laur, head of the custom cloning core at Emory University, and Paolo Colombi...University. Oskar Laur, PhD runs Emory University DNA Custom Cloning Service since 2009. He has  developed a...he has used for cloning of more than 10,000 DNA custom constructs. His work was acknowledged in hundreds...

...part of the recognition sequence, they can be customized to direct assembly of DNA fragments. When designed...utilize the BsaI and BsmBI Type IIS sites such that custom TAL arrays could be constructed quickly and efficiently...Nov 5. PubMed. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for...

...chose needed to fulfill certain requirements: Customizable to our specific needs Modern and well-supported...the order and usage of the scripts needs to be customized in the pipeline for the specific needs of the...Since each process can use a unique container, we customized each container for its particular purpose and...

...one-pot reaction. Using this system, you can create custom RNA binding proteins in as little as 3 days. Abil...Abil et al., 2014 show that you can fuse custom PUF proteins to a translational repressor to down-regulate...Rajarshi, et al. "Engineering RNA endonucleases with customized sequence specificities." Nature communications...