...nuclease-deficient Cas9 (dCas9) protein and a customized s ingle g uide RNA (sgRNA) designed with a 20...with ampicillin resistance, wherein the N20 can be custom designed to target arbitrary sequences in the genome...controlled sgRNA cassette, wherein the GN19 can be custom designed to target sequences in the genome. The...fusion expression plasmid 44248 pgRNA-humanized A customizable gRNA expression plasmid for use with either ...editing bacterial genomes 44251 pgRNA-bacteria A customizable gRNA expression plasmid for use with the bacterial...

... spring of 2011, the groups describe a set of customized plasmids that can be used with the recently developed...plasmids, which are available as a kit from Addgene , custom arrays consisting of 12-31 repeats can be assembled...here . References Efficient design and assembly of custom TALEN and other TAL effector-based constructs for...697-8. Pubmed PMID: 21822241 . TAL effectors: customizable proteins for DNA targeting Bogdanove AJ, Voytas...

...References Rapid 'open-source' engineering of customized zinc-finger nucleases for highly efficient gene... (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML et al. Nat Protoc...19798082 . Targeted mutagenesis in zebrafish using customized zinc-finger nucleases. Foley JE et al. Nat Protoc...

... pairs. Binding specificity is determined by customizable arrays of polymorphic amino acid repeats in ...one to efficiently assemble TALEN constructs with custom repeat arrays. TALEN Kits TALEN Kit Description... (TAL Orthongal Repressors) that can be used to custom repress gene expression in yeast. TALORs consist...

...listed below ( pDR274 ) can be used to construct customized gRNAs targeted to a sequence of interest. Potential...Support Tools” heading. Completed vectors express a customized guide RNA from a T7 promoter. Individual plasmids...

...listed below ( MLM3636 ) can be used to construct customized gRNAs targeted to a sequence of interest. Potential...Support Tools” heading. Completed vectors express a customized guide RNA from a U6 promoter. Individual plasmids...

... (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML, Thibodeau-Beganny...

... as HIV, murine leukemia virus, and vesicular stomatitis virus G) are packaged as replication-defective...leukemia-based retroviral particles Vesicular stomatitis virus particles See Addgene's Viral Plasmids ...

...type II bacterial CRISPR system to function with custom guide RNA (gRNA) in human cells: this involves ...Table 1 We provide a collection of reagents for custom CRISPR mediated gene-targeting. A protocol for ...

...oncogene homolog Muscle RAS oncogene homolog Neuroblastoma RAS viral (v-ras) oncogene homolog RASA RASA1...RASSF10 Ras association domain family member RB1 Retinoblastoma 1 RCE1 Ras converting CAAX endopeptidase 1 ...

...for work in C. elegans . The protocol for customizing the sgRNA expression plasmid can be located ...

...the manuscript for the protocol on assembling customised sgRNA expressing plasmids. Questions regarding...

... sequences of interest can be made by cloning customized oligonucleotides into pSQT1313 using the protocol...

...like GFP, researchers have turned dCas9 into a customizable DNA labeler compatible with fluorescence microscopy...

...resources you may find helpful. Our Scientific and Customer Support Teams are also available to provide assistance...

... deposited at Addgene. We are unable to perform custom AAV preparations for specific gene inserts or plasmids...

...enhancer in B-cells inhibitor, epsilon IKBE NRAS neuroblastoma RAS viral (v-ras) oncogene homolog ALPS4, N-...- NODAL nodal homolog (mouse) MGC138230 NOV nephroblastoma overexpressed gene CCN3, IGFBP9 NPFF neuropeptide...calcineurin-dependent 4 NF-ATc4, NFAT3 NRAS neuroblastoma RAS viral (v-ras) oncogene homolog ALPS4, N-...

... described in Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis...

...molecules or specific genes are also available. Custom biosensors can also be constructed using the cpFRET...

...control by Addgene before being distributed to customers. Details about our production protocols, titering...