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We narrowed to 10 results for: tet rtTA

Showing: 1 - 10 of 10 results
  1. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...is the tetracycline (Tet)-regulated system. You can choose either the Tet-off system or the Tet-on system... interest (pTet-IRES-EGFP, pPRIME-Tet-GFP-FF3) and one with either tTA or rtTA (FUW-M2rtTA). In this kind...system. In the Tet-off system a Tet-response element (TRE) is placed upstream of the promoter in your transfer...expression. In contrast, in the Tet-on system, tTA has been modified (and renamed rtTA) to bind TRE only in presence...expression kinetics as compared to the Tet-off system. One drawback of the Tet system is that it often requires... In the absence of tetracycline or a derivative such as Doxycycline (Dox), the Tet-controlled transactivator...vector systems containing both your transgene and tTA/rtTA (pINDUCER20, pINDUCER21) have been developed, but...
  2. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...lentivirus for delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can serve as controls...experiments, while rtTA can be used to activate expression of a gene under the control of the tet operator. AAV...
  3. Tetracycline Inducible Expression

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    Collection
    ...promoter Tet-On 3G rtTA Bong-Kiun Kaang 20342 FUW-M2rtTA Lentiviral Tet-On vector, expressing rtTA from UbC... of tet-responsive systems: Tet-Off and Tet-On. Read on to learn more about the components of tet systems... center: Tet-Off; right: Tet-On. TRE: Tet response element; tTA: tetracycline-controlled transactivator...expressed occasionally, use Tet-On: express an rtTA or TetR and include a tet-responsive promoter upstream...expression of EGFP Tet-On 3G rtTA TRE3GS Xiaojun Lian 171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for...CMV promoter Tet-On 3G rtTA Oskar Laur 96963 pCAG-TetON-3G Mammalian expression of the Tet-On 3G transactivator...transactivator Tet-On 3G rtTA Elena Cattaneo 104543 PB-EF1a-TetOn3G PiggyBac vector expressing Tet-On 3G transactivator...
  4. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...inducible promoters. The positive inducible tetracycline ON (Tet-On) system, a versatile tool developed for...activation. In this system, the activator rtTA (reverse tetracycline-controlled transactivator) is normally...Addgene.org: Inducible Promoters for Bacteria Tetracycline (Tet) Inducible Systems at Addgene Cre-lox Guide...lot. If you’re looking for high inducibility, the Tet-On system may be a good choice, as it’s documented...require only the addition of an external inducer, like Tet systems, may be activated very rapidly. In contrast...and cannot bind the tetracycline response elements (TRE) in a promoter. Tetracycline and its derivatives...
  5. Retrovirus Plasmids

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    Collection
    ...Vignali 27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA. Expresses rtTA. See Zuber et al. (2011) for...William Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in...
  6. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...has used an inducible MitoTimer reporter using a Tet-on system (pTRE-Tight-MitoTimer) to show its usefulness...cells stably expressing pTRE-Tight-MitoTimer and rtTA. The cells were pulsed with 2µg/ml of Doxycycline...repressor plasmids from Scot Wolfe's lab. These include Tet-inducible CRISPR activators and repressor plasmids...
  7. Lentivirus Plasmids

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    Collection
    ...selection. Christophe Benoist and Diane Mathis 21915 Tet-pLKO-puro 3rd Inducible expression of shRNA with ...EF-1a-driven GFP and shRNA under the control of a tet-responsive H1 promoter. Didier Trono 11651 pLVUT-...puromycin selection. Ie-Ming Shih 25737 pSLIK-Hygro 3rd Tet-based inducible shRNA or cDNA expression, gateway...additional variants. Iain Fraser 15948 pLOVE 3rd Tet inducible gateway destination plasmid for cDNA expression...additional variants. David Root 44012 pInducer20 3rd Tet-inducible lentivirus for ORF expression, multicistronic...plasmid 21374 for dTomato version. Bryan Welm 21916 Tet-pLKO-neo 3rd Inducible expression of shRNA with neomycin...171123 pLVX-TetOne-Puro-GFP 3rd Dox-inducible expression of GFP. Jason Sheltzer 20342 FUW-M2rtTA 3rd Contains...
  8. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...188572 Tet-off TauRD (P301L/V337M) MAPT Myc TRE Parkinson's, FTD Franz-Ulrich Hartl 188573 Tet-off Full...GST-TEV-TBK1 TBK1 polH ALS Sascha Martens 209078 piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120...GFP TRE Parkinson's Vikram Khurana 209080 piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123...GFP TRE Parkinson's Vikram Khurana 209081 piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (...Galloway 235303 pLentiX1-[TRE3G-FXN-P2A-mRuby2-bGH]-EFS-rtTA-P2A-mGL-WPRE FXN mRuby2 TRE3G Friedreich ataxia ...TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-EFS-rtTA-P2A-mGL-WPRE FXN mRuby2 TRE3G Friedreich ataxia ...TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-EFS-rtTA-P2A-mGL-WPRE FXN mRuby2 TRE3G Friedreich ataxia ...
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