We narrowed to 23 results for: tetracycline resistance

A brief summary of the history of plasmids and their use in cloning and molecular biology.

Learn about the importance of antibiotic resistance genes in plasmids. How they work, how to use them, and some great tips.

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

Learn more about the common E. coli strains used in the lab.

Learn more about E. coli strains used for protein overexpression.

Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- or dox-inducible systems.

The next post in the Plasmids 101 series focuses on the promoter region, the element responsible for initiating the transcription of your insert into RNA.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Use the deposit spreadsheet to quickly deposit multiple plasmids with Addgene.

Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids.

Find a list of universal sequencing primers and other common sequencing primers.

Learn about the common techniques to creating engineered mouse models at embryonic stages and the pros and cons of different genome editing techniques.

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

Gamma-retroviral vectors and lentiviral vectors can be used in a wide variety of experiments including those involving genome engineering, RNAi, and inducible gene expression. In this post, learn about some of the most popular viral vectors available from Addgene and their uses.

Mycoplasma contamination? Find out where it comes from, how to detect it, prevent it, and remove it from your tissue culture experiments.

Joanne Kamens provides highlights from the 2015 Canton Nucleic Acids Forum (CNAF).

A selection of Addgene's empty vector backbones curated by species, epitope tags/fusion proteins, function, selectable markers, and more.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

Addgene lentiviral packaging, envelope, and transfer plasmids

Protocol for Inoculating a Bacterial Culture