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We narrowed to 9 results for: tetracycline transactivator

Showing: 1 - 9 of 9 results

  1. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ..., Gossen and Bujard created the tetracycline-controlled transactivator (tTA). They fused TetR with the...its native context, the tetracycline repressor (TetR) can bind to the tetracycline operator sequences (TetO...by Gossen and Bujard, both the promoter and transactivator components of the system have been engineered... in a similar manner to the Tet system. The transactivator configuration of the system is a positive repressible...the cmt and cym bacterial operons. A chimeric transactivator, cTA, was created by fusing CymR to the activation.... Riabinina et al. also developed chimeric transactivators GALQF and LexAQF, which activate their respective...preventing transcription. In the presence of tetracycline (Tet), TetR preferentially binds Tet over the...

  2. Tetracycline Inducible Expression

    Type
    Collection
    ...in the presence of tetracycline. A new reverse tetracycline-controlled transactivator (rtTA) was created...generations of transactivators and promoters are generally cross-compatible. Your choice of transactivator, promoter...Vogelstein Transactivators (tTA or rtTA) Find a construct that expresses the transactivator for your tetracycline...TRE: Tet response element; tTA: tetracycline-controlled transactivator (fusion of TetR with VP16 transcriptional...activation domain); rtTA: reverse tetracycline-controlled transactivator (fusion of mutated TetR with VP16... preps? Find in-stock viral preps of tetracycline transactivators and inducible tools or consider our ...service available for many transactivator or tet-inducible vectors. Tetracycline-inducible Backbones Find...

  3. AAV Molecular Tools

    Type
    Collection
    ...Tools Tetracycline Transactivators Affinity Purification Neurophysiology Cell Ablation Tetracycline Transactivators...These AAV encode tetracycline-inducible tools/controls and tetracycline transactivators that can be used...from Addgene's viral service encoding tet-off transactivators and tools for affinity purification (TRAP)....CAG-driven, constitutive Expression of the tet-off transactivator (tTA) 2 Gradinaru 99120 pAAV-ihSyn1-tTA Inducible... promoter (ihSyn) Expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified...ihSyn) Cre-dependent expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified...used with tetracycline (tet)-inducible expression systems . ID Name Expression System Activity Serotype...

  4. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...reverse tetracycline-controlled transactivator) is normally inactive and cannot bind the tetracycline response... inducible promoters. The positive inducible tetracycline ON (Tet-On) system, a versatile tool developed...response elements (TRE) in a promoter. Tetracycline and its derivatives serve as inducing agents to allow...Addgene.org: Inducible Promoters for Bacteria Tetracycline (Tet) Inducible Systems at Addgene Cre-lox Guide...

  5. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...or off depending on what tet transactivator is used. UAS General expression mRNA Yeast promoter containing...splice acceptor. TRE General expression mRNA Tetracycline response element promoter Inducible with Tetracyline...promoter with low basal activity and several tetracycline operators. Transcription can be turned on or...

  7. AAV Viral Preps

    Type
    Collection
    ...Spagehetti Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation...

  8. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...absence of tetracycline or a derivative such as Doxycycline (Dox), the Tet-controlled transactivator (tTA, ...used inducible lentiviral vector system is the tetracycline (Tet)-regulated system. You can choose either...
Showing: 1 - 9 of 9 results