Plasmids 101: Repressible Promoters
Type
Blog Post
..., Gossen and Bujard created the tetracycline-controlled transactivator (tTA). They fused TetR with the...its native context, the tetracycline repressor (TetR) can bind to the tetracycline operator sequences (TetO...by Gossen and Bujard, both the promoter and transactivator components of the system have been engineered... in a similar manner to the Tet system. The transactivator configuration of the system is a positive repressible...the cmt and cym bacterial operons. A chimeric transactivator, cTA, was created by fusing CymR to the activation.... Riabinina et al. also developed chimeric transactivators GALQF and LexAQF, which activate their respective...preventing transcription. In the presence of tetracycline (Tet), TetR preferentially binds Tet over the...