... Plasmids! Light controls the activity of a microtubule-associated protein to regulate cell growth dynamics...light by modifying EB1. EB1 normally sits on microtubules to attract other proteins involved in their ...into two halves (Fig. 1): one containing the microtubule binding domain and the second containing the ...modified EB1 protein are overexpressed in cells, microtubule growth can be switched off with blue light which...

... Limit tube transfer - the fewer opportunities your viral particles have to stick to your tubes, the higher...siliconized tubes to store samples as it is more difficult for viral particles to adhere to these tubes. Rinse...

...Streptococcus pneumoniae. Robert Husson lab's M. tuberculosis CRISPRi plasmids A group from the Husson lab... used CRISPRi to study essential genes in M. tuberculosis. dCas9 and a gRNA are expressed on pRH2502 and... (Singh et al. 2016). Sarah Fortune's lab M. tuberculosis CRISPRi plasmids Noting that the more common... for transcriptional silencing Mycobacterium tuberculosis and Mycobacterium smegmatis. In addition to ...Investigating essential gene function inMycobacterium tuberculosisusing an efficient CRISPR interference system. Nucleic...

...our guest blogger Kutubuddin Molla from the Pennsylvania State University Kutubuddin Molla is an Ag-biotechnologist...This post was contributed by Kutubuddin Molla, a Fulbright Visiting Scholar at the Pennsylvania State... Institute, Cuttack, Odisha, India. Currently Kutubuddin is a Fulbright Visiting Scholar at the Pennsylvania...

...way! Many thanks to guest blogger Kaustubh Kishor Jadhav! Kaustubh Kishor Jadhav, MSc is a Research Assistant...This post was contributed by guest blogger, Kaustubh Kishor Jadhav, a Research Assistant at MGMs Institute...lowered pH due to microbial contamination. Photo by Kaustubh Kishor Jadhav. Mycoplasmas can remain in the...

...used to convey the difference between a gradient tube before and after ultracentrifugation so that a scientist...each layer. The right panel illustrates a gradient tube before and after ultracentrifugation. The viral ...the bevel pointed up. A syringe at the top of the tube allows air to enter the column so the virus will...

...method procedure The procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant...are mixed with 3 ml of 2X DMEM in a 50 ml sterile tube (for 60 mm dishes only 1.5 ml of each are used)....agarose plug by rapid pipetting, as shown in the YouTube video. Before returning the plate to the incubator...

... K, et al (2015) Resolving bundled microtubules using anti-tubulin nanobodies. Nat Commun 6:7933. https... start re-conceptualizing actin filaments or microtubules. This issue is shared by other SRM techniques...

...same tube. You can clone up to 4 DNA fragments, in a specific order and orientation, in one tube, into...possible to set up the BP and LR reactions in the same tube, speeding up the cloning of the attB-PCR products...

...growing tips of microtubuli and plasmids tagged with MapTau to label entire microtubules. Find these fluorescent...best motif was from the Lck gene. For marking microtubules of mammalian cells, the author found that too...

...TSLP thymic stromal lymphopoietin - TUBB3 tubulin, beta 3 MC1R, TUBB4, beta-4 TXLNA taxilin alpha DKFZp451J0118...

...right, researchers of the world. Cap your Eppendorf tubes, hang up your pipettes, and seal up those plates...decorated their labs with everything from Falcon tube trees to giant paper mache Krampus performing routine...

...your experiment results! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient ultracentrifugation...video content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources on the Addgene blog...

...to sit down and talk with us. Tom McFadden is a YouTuber and science educator looking for creative ways...communicate science. Learn more about Tom on his youtube channel and at sciencewithtom.com.   Special thanks...

... safely recycle these materials either.  Test tubes aren’t cheap everywhere Lab supplies and equipment...Coast where her first job was to wash Eppendorf tubes which were to be used for 3 months before they could...

... your final tube after purification. How do you know if you actually have DNA in your tube without seeing...

...chemical fuel sources. Like an actin filament or a microtubule, R bodies are a polymer of many copies of monomeric...ectosymbionts of Euplotidium (Ciliophora) that contain microtubule-like structures are bacteria related to Verrucomicrobia...

...time needed to prep for an experiment (labeling tubes never takes longer than 5 minutes, right?) As a ...   Visual Planning  Plate maps, pre-labeling tubes, and even drawing out experimental steps are worth...

...tested whether or not FP-tubulin fusion proteins could properly form microtubule networks in cells. Other...

...many different things do you simply refer to as a “tube”? We took steps towards solving this problem by...Prescribing an official name to all of our plates, tubes, and racks meant we had fewer errors in our LIMS...