...Ahringer Alexander Stark Bas van Steensel Richard Young Yi Zhang Leonard Zon  You!     Chromatin-related.... Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M, Perez M, Agarwal P, Brodbelt JS...

...Mobile-CRISPRi plasmids at Addgene! References Auchtung, Jennifer M., et al. "Biology of ICEBs1, an integrative... 86 (2016): 14-25. PubMed PMID: 27381852. Choi, Kyoung-Hee, et al. "A Tn7-based broad-range bacterial ...

...modified version of the Python program described by Joung et al. 2017, which we hope to make available as ...:664–678 . https://doi.org/10.1089/hum.2019.277 Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt...

...This guest post was contributed by Johnny Kung, Director of New Initiatives for the Personal Genetics...public on the implications of their work. Johnny Kung is the Director of New Initiatives for the Personal...

...insert FLAG tags into the genome of the pathogenic fungus Aspergillus fumigatus, which has been difficult...microhomology-mediated end joining in Aspergillus fumigatus.” Fungal Genet Biol. 86: 47-57. PMID: 26701308. Additional...

...retired! Conversely, cells younger than the 3rd – 4th passage are too young and should not be used for...

... as anthocyanin production, or disease, such as fungal growth. Healthy plants produce lots of seeds. More...mean more chances to recover a transformant. Use young plants: If you transform early, your plants will...

...model organisms, including bacteria, alternative fungi (C. albicans, pC2HB) (7) and mammalian cells. If...codon adapted two-hybrid system for the pathogenic fungus Candida albicans. Nucleic Acids Res. 2010;38(19...

...Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing with CRISPR-Cas13....Park SW, Kim D, Kim K, Cho H-Y, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim J-S (2017) In vivo...

...overcome this problem in C. roseus by working with young seedlings and optimizing several other aspects of...re-invent the wheel. Test different leaf ages Usually younger leaves work better. Not every leaf is equally susceptible...

...within cell-free genomic DNA, and detect COVID-19 (Joung, Ladha et al., 2020). Cas13a in mammalian cells ... 26593719. PubMed Central PMCID: PMC4660269. 7. Joung J, Ladha A, et al., ‘Point-of-care testing for COVID...

...14704360. PubMed Central PMCID: PMC373311. 3. Kim, Jung-Sik, et al. "Epitope tagging of endogenous genes...18784188. PubMed Central PMCID: PMC2577350. 4. Kim, Jung-Sik, et al. "Intact cohesion, anaphase, and chromosome...

...TALengineering Reagents Joung Lab TAL Effector Engineering Reagents You may also like... Keith Joung Lab plasmids...Reagents from the Keith Joung laboratory for engineering TAL effectors, including designed TALENs, and...plasmids Dan Voytas Lab plasmids CRISPR plasmids The Joung Lab has developed three platforms for engineering...pages describing these reagents are provided below: Joung Lab REAL Assembly TALEN Kit Individual REAL TALE...

..., J. D. Sander, J. Staber, S. J. Wheelan, J. K. Joung, P. B. McCray, F. D. Bushman, P. L. Sinn, and N....Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient iPSCs.,”...

...the procedures in the lab are done with robots. Young scientists going into this field don’t really get...position in academia. It is therefore important for young scientists and PhD students to also explore a range...

...Corsino. When someone says “internationalization” young hearts often shake with the fear of uncertainty....entrepreneurial visions thereby opening new frontiers for young talent around the world. The wealth of knowledge...

...this represents a major downside in optogenetics (Belzung et al., 2014). This could be overcome by fusing...28.28 (2008): 7025-7030. PubMed PMID: 18614669. Belzung, Catherine, Marc Turiault, and Guy Griebel. "Optogenetics...

... a nucleotide triplet and when several ZFNs are strung together, it can read many base pairs simultaneously...Pickle CS, Ralston EJ, Lee AH, Amora R, Miller JC, Leung E, Meng X, Zhang L, Rebar EJ, Gregory PD, Urnov ...

...the first ever CRISPR-based clinical trial in 18 lung cancer patients. This study used CRISPR gene editing...for acute lymphoblastic leukaemia in children and young adults: a phase 1 dose-escalation trial." The Lancet...

...Joung lab Dimeric CRISPR RNA-guided FokI Nuclease (RFN)... CRISPR Joung Lab CRISPR Plasmids CRISPR-FokI ...FokI Nuclease ( RFN ) technology developed by the Joung lab ( Tsai et al., Nat Biotechnol. 2014 ). Use of...Biotechnol. 2014 . The ZiFiT Targeter program from the Joung lab can be used both to identify potential target...