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.... This is why they are called transferable skills. Why are transferable skills so important? It is important...   What is a transferable skill? Time management. I needed it when balancing a handful of demanding courses...much in my current role as Customer Support and Operations Manager at Addgene. In this role, I balance my...important to identify which transferable skills you possess because any potential employers, collaborators... examine not just technical skills, but also transferable skills, before offering someone their dream ...certainly matters in the job market, it is the transferable skills that create a full package and add an...for that ideal job. How can you identify your transferable skills? PLoS One recently published an article...

...feel they lack formal training in a variety of transferable skills. At Addgene, we've set out to fill this... by highlighting that researchers learn many transferable skills while working in the lab and by offering...where you might need some help. Today in our transferable skills guide: Negotiation. As a grad student...Hawaii?). So how can you approach improving this transferable skill? Negotiation as a zero sum game Some ...negotiation skills You can easily work on this transferable skill without any formal training or coursework...business development team, negotiation is a key transferable skill that can help you get more of what you...resources on the Addgene blog More from the transferable skills guide: Conflict Resolution Time management...

...into the field of gene therapy, more specifically AAV-based retinal gene therapy. The year was 2001 and...for gene (and cell) therapies. One may wonder why the first AAV-based gene therapy treatment for a genetic...forward with ocular gene therapy The pipeline of AAV-based ocular gene therapies is impressive: clinical...candidate therapies for these diseases. Riding on the early successes of retinal gene therapy, an ever-growing...further reading 1. FDA approves gene therapy for blindness 2. Gene Therapy emerges from disgrace to be the ...Stock of Retinal Gene Therapy: Looking Back and Moving Forward. Molecular Therapy Vol. 25 No 5. PubMed ...Muzyczka N., 2014. AAV-Mediated Gene Therapy for Research and Therapeutic Purposes. Annu Rev Virol. 2014 Nov...

...iPSCs, the the ability to generate teratomas is the gold standard instead. Teratoma formation requires injecting...almost all available approaches for generating iPSCs. The generation of iPSCs is relatively simple in concept...delivery methods Overall, integrating viral vectors efficiently and reliably generate iPSCs, but they ...transposase. A challenge of using PB to generate iPSCs is that genomic alterations can occur at the transposon insertion...has lower levels of efficiency at generating iPSCs, usually several orders of magnitude lower than retroviruses...making chimeras by injecting iPSCs into blastocysts and implanting them into female mice. Generating chimeric...Gene-delivery systems for iPS cell generation. Expert opinion on biological therapy, 10 2, 231-42. PubMed PMID...

...codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available from the ... NanoLuc® Luciferase-based tools are fantastic examples of technologies that can accelerate your research... creation of NanoLuc® Luciferase from a rather dull 19kDa Oplophorus luciferase by Promega’s Advanced ...than our more common luciferases like firefly (FLuc) and Renilla (RLuc) luciferase. This is important not...coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, to produce...Promega supports the Addgene mission statement: Accelerate research and discovery by improving access to...bright enough to detect endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is...

...Blog Transferable Skills Guide: Problem-Solving Transferable Skills Guide: Teamwork Transferable Skills...feel they lack formal training in a variety of transferable skills. At Addgene we're helping to fill this...both helping researchers recognize their MANY transferable skills learned while working in the lab and ...Today, we’re highlighting an oft-underlooked transferable skill: creativity. I’ve heard quite a few scientists..., it’s clear that creativity is absolutely a transferable skill developed during PhD training. Creativity...to do this and are still struggling with idea generation, try this thought exercise: Consider the issue...three-ring binder as a starting point, the ideas generated were hardly novel. When told to use a roller skate...

...proteins being tested for interaction. If no interaction occurs, the luciferase emits its characteristic...conformation of a single protein. Considerations for luciferase assays Luciferases can only produce light in ...Well-established Natural and engineered luciferases Although firefly luciferase was the first bioluminescent reporter...have engineered a variety of improved luciferases. NanoLuc® Luciferase is the best known: it’s derived from...Oplophorus gracilirostris) luciferase and engineered to be extra bright. Other luciferases have been modified ...mammalian systems. Our luciferase collection page has a detailed list of luciferase options. Table 2: Properties...ATP No Luciferase reporter assays for monitoring gene regulation Since firefly luciferase was first...

...work as well. Social interaction is a good thing Part of you needs to interact with other people, whether...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this... highlighting that researchers do learn MANY transferable skills while working in the lab and by offering... just Googled the phrase “People need social interaction” and received 104 million results (Here’s the...my bedroom. I realized I could and should be interacting more with other people, and becoming part of ...on the Addgene Blog Read other posts in the Transferable Skills Guide Management for Scientists: Managing...

...to study RNA-protein interactions? The characterization of RNA-protein interactions has lagged behind, ...identifying protein-protein interactions, but RaPID identifies RNA-protein interactions. Both methods rely on...detect a known RNA-protein interaction.  In as little was one minute BASU generated a strong streptavidin ...Blog Study Protein-DNA Interactions with CUT&RUN Study Protein-Protein Interactions with Split-BioID Find...attention.There are numerous ways to detect DNA-protein interactions or to analyze chromatin states (CHIP-seq, FAIRE-seq...FAIRE-seq, Cut & Run) and to detect protein-protein interactions (yeast-two hybrid, Co-IP, BioID),  and that’...limitations of current means to detect RNA-protein interactions. To address this need, the Khavari lab at Stanford...

...Page Read Other Posts in the Transferable Skills Guide Developing Transferable Skills During Science Training...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this... highlighting that researchers do learn MANY transferable skills while working in the lab and by offering...where you might need some help. Today in our transferable skills guide: Collaborating with others outside... Addgene as a nonprofit organization is very considerate and thoughtful about its limited resources. Rather... will often look for partnerships where we can leverage another organization’s expertise to our mutual... other projects like it going, someone (likely several people at Addgene) had to take the initiative to...