...costly to share. What is a RANbody? A RANbody is a fusion between a reporter and a nanobody that’s encoded...J.R., & Yamagata, M. (2018). Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical...

.... Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on a reduced glutathione...glutathione matrix. The advantage of a fusion protein is the efficient purification of an already immobilized...for kinetic analysis Proteolytic removal of the fusion protein could provide a more relevant kinetic analysis...produces biotin ligase and a protein of interest fused to maltose binding protein (MBP, New England BioLabs...protein in bacteria and subsequently purify the fusion protein from a bacterial extract. The MBP tag can...using Xa protease. The carboxyl-terminus of the fusion protein includes an AviTag and a 6X HisTag for ...protein we tested, it may be possible with other fusion proteins - but it would remove both the AviTag ...

...Fluorescent Protein Fusions Observing a bright signal for the first time after cloning an FP fusion with your favorite...consequences of misfolded dark FP fusions including erroneous quantitation of fusion protein levels. It remains...misfolding impacts fusion protein function and this could lead to under and overestimation of fusion protein activity...impact of dark, non-fluorescent, and misfolded FP-fusions on quantitative imaging experiments and cell physiology...interest is exciting and rewarding. But, what if an FP fusion with a resident endoplasmic reticulum (ER) protein...troubling, what if a significant fraction of the fusion protein molecules fail to correctly fold? Misfolded...localization and dynamics of fluorescent protein fusions in live cells in real time. Previously, we have...

...engineering of fusion proteins is relatively straightforward. However, generating a fusion while keeping...Creating fusions at the N- and C- termini of your protein of interest The most straightforward fusion protein...use terminology like “C-terminal fusion,” but I find this very confusing because it can mean that the fluorescent...cloning site for generating fusions. For the POI-fluorescent protein fusions, our experience is that a ...For the fluorescent protein-POI fusions, we generate straight fusions, without any linker. When both orientations...example, the APT1-mVenus fusion localizes at the Golgi, whereas the mVenus-APT1 fusion does not (figure 2)....fluorescent protein fusions There are several choices to consider before generating a fusion protein, e.g. ...

... them to a tumor - they used focused ultrasound (FUS) to heat the tumor area, as the image below shows...stable levels for 6 days - after just 15 minutes of FUS.  Similar constructs to the one above can be made...

...events that occur in less that 5% of the population (Fu et al. 2013, Vouillot et al. 2015). Meanwhile, Sanger...sequence requirement at the terminal 3’ PAM site (Fu et al. 2013; Pattanayak et al. 2013). This approach...magazine.scienceforthepeople.org/.     References 1. Fu, Yanfang, et al. "High-frequency off-target mutagenesis...

...guides (19-20bp)?  Small-scale studies (Tsai et al., Fu et al.) suggest 17-19 bp long guides have reduced...PMID: 25513782. Pubmed Central PMCID: PMC4320685. 3. Fu, Yanfang, et al. "Improving CRISPR-Cas nuclease specificity...

...However, Cas9 can be toxic if continuously expressed (Fu et al., 2013). Using IDLVs ensures transient expression...94-007-4899-6_7. PubMed Central PMCID: PMC7120651 Fu, Y., Foden, J. A., Khayter, C., Maeder, M. L., Reyon...

...PMID: 26829887. PubMed Central PMCID: PMC4735761. 6. Fu, Y. et al. High-frequency off-target mutagenesis ...

...10.1016/j.neuron.2010.11.021  Gambarotto D, Zwettler FU, Guennec ML, et al (2019) Imaging cellular ultrastructures...