We narrowed to 740 results for: NSI

...Kebabian, John W., and Paul Greengard. "Dopamine-sensitive adenyl cyclase: possible role in synaptic transmission...Gary L. Petzold, and Paul Greengard. "Dopamine-sensitive adenylate cyclase in caudate nucleus of rat brain...

...equipment needed for EM or SRM? Enter Expansion Microscopy. What is Expansion Microscopy?  ExM provides a straightforward...done about that.  Second, expansion introduces distortions. The goal in expansion is for your sample to expand...maximizing label density. In some cases, nanobodies have been found to help improve label density (Mikhaylova... Bournonville L, et al (2021) Expansion microscopy provides new insights into the cytoskeleton of malaria...Expansion Microscopy (ExM) promises an easier, more accessible way to image biological features previously... ExM images in a new publication.         Why Expansion Microscopy?  Traditional light microscopes are...samples can be expanded to ~4-10x linearly! This expansion physically separates molecules while maintaining...

...investigator who is using an in vivo animal model should consider the possible elicited immune response from viral...

...serve Addgene and ourselves. We are also able to consider different technical approaches that may come up...

...was aware that such roles existed, but did not consider them for myself. I started picturing myself in...technical support specialist for 4 years. Read on for insights from my time in technical support including tips...takes some time (several years) to build up comprehensive lab experience. With this valuable training ...any incoming requests or problems - there is considerable diversity in the types of questions customers...individual stories and get (sometimes refreshing) insights into how other scientists think and work. Most...drive to help others. These roles offer an easy transition from an academic lab to industry without being...

...s post, we’ll discuss some of the important considerations you should think about when developing optogenetics...place aversion also models reinforcement but is considerably simpler than ICSS. The test chamber is divided... of the type of behavior, certain technical considerations still apply. Testing stimulation First, In ...cue. The hippocampus has long been known to be responsible for mediating this behavioral effect but the ...mouse; however, all the steps and technical considerations are directly applicable to the rat [6]. Technical...PMC3150193. 8.Han, Xue, et al. "A high-light sensitivity optical neural silencer: development and application...

...Plasmid Sharing Or read more about what happens Inside Addgene   ...

...development of transient transformation systems for other species as well. Things to consider in developing...generating a transgenic plant can take even longer, a transient plant leaf transformation process could save the...transgenic plant takes even longer, is more labor intensive, inefficient, and often not reproducible. Prior...to creating transgenic cultures or plants, our transient expression method enables us to quickly screen...biosynthetic pathway. Why plant scientists do transient leaf transformations The development of transgenic...common problem in non-model organisms. Therefore, transient methods are usually the first choice for investigating...instead of months to years. In the plant sciences “transient transformation” usual means that neither a stable...

...may be time, then, to look towards genetic code expansion (GCE): techniques in which non-canonical amino...introduced to the bacteria using plasmids. Rapid expansion occurred, with hundreds of new ncAAs and techniques...number of experiments with. The catch The rapid expansion of GCE led to the creation of many new tools and...meaning that the final protocol may involve a sensitive balance of trade-offs. This balance can be extremely...Tan, Z., & Cornish, V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry...

...technique! What is a photosensitizer? First, for a few definitions. A photosensitizer is a dye or protein...system? Turns out that with light-inducible photosensitizers and a quick zap of the proper color light,...light, you can do just that.  Photosensitizing dyes and proteins have been around for awhile (check out this...Bruchez and Tsang labs recently developed a photosensitizer composed of the protein complex complex and... oxygen species (ROS). The ROS produced by photosensitizers inactivate target proteins or cells while ...having little effect on neighboring cells. The photosensitizer used in this work is composed of two parts:...fluorogen (the TAP - targeted and activated photosensitizer - MG-2I in this case). The fluorogen is not...