...became known as SNAP-tag. There is also a fast-reacting version of SNAP-tag called SNAPf. eDHFR: The Virginia...Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry, 60(33), 2560–2575...than short peptide tags like tetracysteine (Jing & Cornish, 2011). HaloTag and SNAP-tag are the most popular...application, SNAP-tag, CLIP-tag (both 182 aa), and eDHFR (159 aa) are smaller than HaloTag (295 aa) – for...on resorufin). SNAP-tag: In 2003, Kai Johnsson’s lab developed a self-labeling fusion tag based on the ...Although based on the same original enzyme, SNAP-tag and CLIP-tag show good specificity for their own ligands...genetic tag on the protein of interest. Table 1: Summary of popular self-labeling tag systems Tag Size...

...resistance genes, selectable markers, promoters, origins, tags, and certain ORFs. Their sophisticated detection...powered by GSL Biotech's SnapGene Server Software. With the backing of SnapGene’s sequence viewer software... Overview of SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid...the features you need quickly. SnapGene’s Feature Library - SnapGene’s software seamlessly identifies ...example below. Our old map is on the left while the SnapGene powered map is on the right (click here to see...t detected by our in-house software. In fact, SnapGene’s feature library includes nearly 3x as many features...identify. Improved Restriction Enzyme Handling - SnapGene’s software positions restriction enzyme annotations...

...markers, tags, origins of replication, and fluorescent proteins that are already included in Snapgene’s extensive...through our Snapgene powered maps and sequence analysis tools.     The main ways we use SnapGene for plasmid...version of Snapgene to visualize the sequence and look for features. The first thing that the Snapgene software...software as the desktop version of Snapgene. When you download a Snapgene file directly from our website,...website, you can open it with either the Snapgene viewer or Snapgene Desktop programs to analyze the sequence... plasmids. We’ve also talked about how our new Snapgene generated maps provide improved feature detection...with an easy to use interface. We regularly use Snapgene for our quality control process because of its...

...Twelvetrees Lab took advantage of the self-labeling tags SNAPf and CLIPf. These tags rely on synthetic dye...double-tagged constructs (including "CLAP", combining an N-terminal CLIPf tag and a C-terminal SNAPf tag;...10.1101/2024.02.11.579803).  CLIP, CLAP, SNAP! Self-labeling tags for single-molecule biophysics By Mike...Figure 2: CLIP-SNAP fusion proteins for single-molecule FRET. A) Schematic of a CLIP-SNAP fusion protein...dicots Self-labeling tags for single-molecule biophysics Fast and responsive voltage indicator ONE-GO biosensors...constructs for tagging the kinesins KIF5A and KIF5B as well as backbones for generating other SNAPf-, CLIPf-...CLIPf-, and CLAP-tagged proteins for mammalian cell expression. These plasmids will be useful not only for...

... who uses SnapGene or the free SnapGene Viewer companion product. If you don’t use SnapGene yet, you can...guest bloggers Aline and Benjamin Glick from SnapGene. SnapGene was created to meet a need. While there were...approach is SnapGene’s algorithm for detecting common features. This algorithm enables one of SnapGene’s most... us at snapgene.com. Many thanks to our guest bloggers Aline and Benjamin Glick from SnapGene!  Ben Glick...Addgene Blog Read about our SnapGene powered plasmid maps Learn how we use SnapGene during the plasmid quality...properties of the DNA molecules they were using. SnapGene was created to alleviate these problems through...make specialized controls available when needed. SnapGene has been engineered to be easy and enjoyable to...

...wintertime festivities! Help us spread some cheer by snapping a photo of you and your labmates celebrating the... the holidays and tweeting it with the tags #DeckTheLab and @Addgene. Christmas, Hanukkah, ugly sweaters...you name it, we want to see it.      All photos tagged with #DeckTheLab throughout the month of December...

... Why #DecktheLab? Help us spread some cheer by snapping a photo of you and your labmates celebrating the... the holidays and tweeting it with the tags #DeckTheLab and @Addgene. We’d love to see how you’re decorating...Festivus— you name it, we want to see it! All photos tagged with #DeckTheLab throughout the month of December...

...other fluorescent proteins and tags including mCherry, HaloTag and SNAP-tag for multi-color imaging. Find...observed colocalization of GFP tagged HA frankenbody with an mCherry, 4xHA tagged histone protein (H2B) in ...could also bind a 1xHA tagged histone protein as well as a mitochondrial protein tagged with spaghetti monster... the 10xHA tag increased the fluorescent signal to noise ratio five fold over the 1xHA tag.  Lastly, the...use GFP to image nascent proteins. In addition GFP tags are large, dim, and permanently attached to a protein...limitations, scientists have co-opted antibodies for tagging purposes. Different antibodies bind different protein... called epitopes and scientists can use these to tag their proteins of interest. Antibody based probes...

... with containing chemical tags such as the SNAP-tag, Halo-tag, and CLIP-tag (Kohl et al., 2014) (Addgene... et al. "Ultrafast tissue staining with chemical tags." Proceedings of the National Academy of Sciences111.36... circuits with fluorescent proteins and chemical tags." eLife 7 (2018): e40350. PubMed PMID: 30454553....

...frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and HaloTag fusions, while the anti-HA frankenbodies... small epitope tag (FLAG is eight amino acids, HA is nine), multiple repeats of the tag can recruit multiple...b) Schematic of anti-FLAG-FB-GFP binding to FLAG-tag H2B for nuclear localization. (c) Applications of...blotting, and more. Given the popularity of FLAG and HA tags, you may not even need to clone new constructs to.... The proteins, which are purified via histidine tags, are optimized for SDS-PAGE gels and can be detected...frankenbodies include mEGFP, mCherry, and HaloTag. Figure 1: (a) Anti-FLAG frankenbody (FB) construction from...editing within a single expression cassette is advantageous to Cas9’s larger expression requirements (Figure...