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We narrowed to 602 results for: ACE

Showing: 1 - 50 of 602 results

  1. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...Synthetic Biology.” ACS Synthetic Biology, vol. 5, no. 10, American Chemical Society (ACS), 2 May 2016, pp...Traditional Biology.” ACS Synthetic Biology, vol. 5, no. 1, American Chemical Society (ACS), 4 Nov. 2015, pp...Secretion Libraries.” ACS Synthetic Biology, vol. 6, no. 6, American Chemical Society (ACS), 15 Mar. 2017, ...Multipart Assembly.” ACS Synthetic Biology, vol. 4, no. 9, American Chemical Society (ACS), 1 May 2015, pp...Saccharomyces Cerevisiae.” ACS Synthetic Biology, vol. 12, no. 11, American Chemical Society (ACS), 6 Nov. 2023, ...Toolbox for Plants.” ACS Synthetic Biology, vol. 3, no. 11, American Chemical Society (ACS), 20 Feb. 2014, ...Acinetobacter Baumannii.” ACS Synthetic Biology, vol. 12, no. 9, American Chemical Society (ACS), 16 Aug. 2023, ...

  2. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...epitopes of interest that are found in the extracellular space. The targeted epitopes can be motifs within...Note that intracellular labeling must be performed only after viability staining and surface labeling ...cell population. To detect non-surface markers, a separate intracellular labeling protocol is necessary...chemical treatment of the cells after surface labeling. Intracellular labeling can be performed on cytosolic...cytometric analysis, the most common method is a cell surface label, where fluorophore-conjugated antibodies ...on those proteins, like glycosylation patterns. Surface labeling is particularly useful for classifying...those techniques can be used alongside standard surface labels. Here, I will introduce you to a few useful...

  3. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...that they may want to consider for future work placements. The chance to learn and collaborate all while...which you can apply to. These structured types of placements often have some form of built-in professional...for success prior to your departure for the new placement. Make sure you read any relevant literature for...

  4. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...almost all of these guidelines can apply to any workplace or job. It amazes me when the most analytic scientists...thing to do is not go into these labs in the first place. This is another reason to do your research and ... Wrote blog, Toxic Academic Mentors.  What is workplace bullying? Threats to professional status – including... Scientists love learning and science so find a place that loves you back. Additional resources on the...

  5. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ... Controlled CRSIPR-Cas-based Genomic Regulation. ACS 6, 5, 695-703 (2020). https://pubs.acs.org/doi/10.1021...is 3’ phosphorothioate linkage, where a sulfur replaces a nonbridging phosphate oxygen. Other useful backbone...

  6. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...orders that are placed through internal purchasing systems. All orders must be placed directly on our ... questions related to the ordering process: I placed an order through my university’s purchasing system...getting at one bigger question: How does a customer place an Addgene order using a PO number? Why do I have... organization can create an Addgene account and place an order: a purchasing agent, lab manager, or the... the contact email addresses provided. How do I place an order using a PO number? Placing an order on ...you’re using a PO number. Follow these steps to place an order online while using a PO number: Log in...

  7. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...interview with Novartis researcher, Carrie Bhang. The ClonTracer Library, deposited by Carrie Bhang, a research... exposure to the drug discovery process.” The ClonTracer library originally came about as Bhang and co-workers...questions that we could apply [it to].” Using ClonTracer to study drug resistance Although Bhang and co-workers...the publication, Bhang and coworkers used the ClonTracer library to determine whether or not cancer cell...the total population are easily be missed. The ClonTracer library allows a researcher to label individual...cells' genomes upon infection. When using the ClonTracer system, lentiviruses containing these barcodes...is performed via barcode sequencing. Using the ClonTracer library, Bhang et al. found that antibiotic resistant...

  8. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...enthusiastic, “Our company culture! Addgene is the best place to work, we do SO many fun things!!”  Okay - honestly.... Instead we picniced outside of our new office space on the nicely manicured lawn of University Park ... the difference here at Addgene is the emphasis placed on team building throughout the years. This team... master drywall hanger?  4. Utilize your office space too Balance point number 3 with doing fun things...

  12. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...They called this editor Target-ACE (adenine and cytosine editor). Target-ACE exhibits increased editing efficiency...mammalian cells. Read the paper in bioRxiv Find Target-ACE   CRISPR/Cas9-based toolkit for epitope tagging ...this toolkit might be used in. Read the paper in ACS Synthetic Biology Find the CRISPRi toolkit SECURE...ribonucleoproteins for transient expression. They replaced the tetraloop of the sgRNA scaffold with a com...

  13. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ...Chemical Biology 2018. PubMed: PMID 30220597. ACE system: A versatile in vivo fluorescent reporter ...monitors both APOBEC- and Cas9-mediated Editing (ACE) in real-time. The reporter constitutively expresses...fluorescence microscopy or flow cytometry. Using the ACE system, the Harris lab showed that human APOBEC3A-Cas9n-UGI...containing rat APOBEC1. Finally, they show that the ACE reporter can also quantify Cas9 cleavage, thus exhibiting...gene editing activities. Click here to find the ACE reporter plasmid! Martin et al., Nucleic Acids Research...

  15. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    Published
    Feb. 2, 2018, 3:20 p.m.
    ...College Park, but she quickly realized it wasn’t the place for her. “I went to grad school with the plan of...different directions is sometimes at odds with the space needed to be creative. “You need a lot of time to...Susan feels she was lucky to have been in the right place at the right time when she started her scicomm career...

  16. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...mediated editing in real time The Harris lab created ACE, a reporter that monitors APOBEC (a family of cytidine...editing in real time (St. Martin et al., 2018). The ACE reporter is a bicistronic construct that consists... Figure 1: APOBEC- and Cas9-mediated editing (ACE) reporter in action. Image from St. Martin et al....expression to cells expressing eGFP and mCherry. ACE was used to identify cells that have been successfully...and APOBEC3B (St. Martin et al., 2018). TLDR: The ACE reporter from the Harris Lab relies on the restoration...for single base editing by APOBEC-Cas9 Though the ACE reporter quantifies APOBEC-Cas9 efficiency in real-time..., and luciferase. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR...

  17. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...a red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory rhodopsin (Ace2N) with the red ...Figure 2: Ace-mScarlet is a fusion between the voltage-sensitive inhibitory rhodopsin Ace2N and mScarlet...seven or eight amino acid linker between them. The Ace-mScarlet voltage indicator works like many FRET-opsin... an increase in the neuron’s membrane potential, Ace absorbance increases and results in higher quenching...blue-light-sensitive channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids at Addgene! Beck...mScarlet. Ace2N quenches a proportion of mScarlet's emission via FRET. Image from Beck and Gong, 2019. ...

  18. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...the ACS career fair is more industry-focused. For another industry-geared option, try the Biospace career...Biology (ASCB) or the American Chemical Society (ACS) are just 2 examples of organizations that hold large...best options are the Naturejobs Career Expo and the ACS career fair, which are larger meetings offering resources...Similar to a conference, job fairs bring you face-to-face with working professionals, with the added benefit...career events page. Biospace holds career events all over the country; sometimes several per month, giving... online career resources Once you’ve identified places you’d like to work, get online and take a look ...know, where they are, and who they know all in one place! This information can be critical to a focused and...

  19. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...spheroplasts to release encapsulated proteins. Video by ACS. For a more cartoony explanation of R-bodies, check... Breaks Membranes to Release Encapsulated Cargo. ACS Synth. Biol. (2016). doi:10.1021/acssynbio.5b00237...penetrate other barriers (like vasculature or extracellular matrices) in response to pH changes. Beyond...functionalized so that they adhere to specific surfaces, or they could be decorated with enzymes for use...

  20. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    .... Created with BioRender.com In their recent ACS Synthetic Biology publication, Mary Dunlop’s lab ...cut in as little as 2 hours.  Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org/doi/10.1021...

  21. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...Figure 1: Ace-mScarlet is a fusion between the voltage-sensitive inhibitory rhodopsin Ace2N and mScarlet...fluorescent output. The GEVI family of gadgets includes Ace, JEDI, and many more. They can be targeted to the...Indicators? Electrical signals are integral to many facets of biology. For example, many heart issues stem...mScarlet. Ace2N quenches a proportion of mScarlet's emission via FRET.  Image from Beck et al, 2019 under ...genetically expressed enzyme, typically on the cell surface, which can act on an upstream component, the chemical...Packaging viruses also have various serotypes – the surface proteins that confer affinity for a target – and...

  22. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...by T4 DNA Ligase and Application to DNA Assembly. ACS Synthetic Biology. https://doi.org/10.1021/acssynbio...52 Parts through Data-optimized Assembly Design. ACS Synthetic Biology, 11(6), 2036–2042. https://doi....interested in complex Golden Gate Assembly, a great place to start is the 24-piece lac operon assembly deposited...

  23. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...aptamer for visualizing RNA–RNA assembly in vivo." ACS synthetic biology 6.9 (2017): 1710-1721. PubMed PMID...high-affinity complex for RNA labeling and tracking." ACS chemical biology 9.10 (2014): 2412-2420. PubMed PMID...challenging to track intracellularly, but aptamers permit the study of intracellular RNA dynamics. Aptamers...then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal cells (Nilaratanakul...simultaneously target cellular RNA to specific intracellular locations using Cas9 and track the localization...is better able to maintain its fluorescence intracellularly due to a lower dependency on magnesium and ... and both TO1-biotin and TO3-biotin contain an acetate modification that reduces nonspecific binding with...

  24. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...shrimp utilizing a novel imidazopyrazinone substrate. ACS Chem Biol 7, 1848-57. PubMed PMID: 22894855. PubMed...for the analysis of protein-protein interactions. ACS Chem. Biol. 10, 1797-804. PubMed PMID: 26006698. ...luminescence. Intracellular Calcium Sensor Yang, J. et al. (7) wished to trigger intracellular Ca++ release...brighter than RLuc8 (6). Susuki, K, et al. therefore replaced the RLuc8 moieties in their Nano-Lanterns with...biosensors utilizing NanoLuc® luciferase Many intracellular sensors of events like calcium release have ...concentrations of biological molecules. FRET sensors face challenges of photobleaching, autofluorescence, ...Luciferase. CalFlux VNT responds to changes in intracellular calcium and is useful for direct imaging of ...

  25. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...https://doi.org/10.1016/j.omtm.2019.01.008  Maurer AC, Weitzman MD (2020) Adeno-Associated Virus Genome...expressed from a separate plasmid in trans, freeing up space in the viral genome so that a gene of interest can...

  26. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...doi.org/10.1126/science.1258096 Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR...39–56 . https://doi.org/10.1085/jgp.36.1.39 Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR (2016) Programmable... authors were exploring were space and robots. Warping to stars in space ships and the rise of killer ...genetic engineering as CRISPR-Cas editing soon replaced the protein-based DNA-binding domain with a single-guide...Into Darkness (2013) which is the remake of the space-melodrama Star Trek II: The Wrath of Khan (1982)... combined the worlds of genetic engineering and space travels. The movie sees the USS Enterprise crew ... Newby GA, Raguram A, Liu DR (2019) Search-and-replace genome editing without double-strand breaks or ...

  27. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...Chesterfield et al., 2020     Chesterfield et al., ACS Synthetic Biology 2020. https://pubs.acs.org/doi/...

  28. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...JG, Glaudin AM, Krumm BE, Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT (2020) TRUPATH,...bound to the intracellular surface of the GPCR. Binding of a ligand to the extracellular surface of the GPCR...environmental conditions, cells must be able to detect extracellular stimuli. One way they do so is through G protein-coupled...converts a signal received by a GPCR on the cell’s surface into action that can result in the initiation of...well known and, as a result, a target of many pharmaceutical drugs, there is still only a partial understanding...

  30. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...Covalent TMP-Tag Based on Proximity-Induced Reactivity. ACS Chemical Biology, 4(7), 547–556. https://doi.org/...Technology for Cell Imaging and Protein Analysis. ACS Chemical Biology, 3(6), 373–382. https://doi.org/... HaloTag for their “ticker tape” method for intracellular recordings, switching between different colored...appearance at the filament tip from (a). c) Intensity traces at the tip of the filament from (a-b), showing ...length (lower). d) Intensity and filament length traces for a different event where CP appears briefly ...ligands! Self-labeling tags can also be used for surface immobilization, purification, or other uses of ...self-labeling protein tags are increasingly popular. Embrace the flexibility and switch things up for your next...

  31. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...reproducing useful natural materials.      Moore et al., ACS Synthetic Biology 202. https://doi.org/10.1021/acssynbio...

  32. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...biosensors and displayed her findings at the Spring 2017 ACS meeting. She hopes these biosensors and her future...that these enhanced “pdDronpa” domains could be placed in amino acid loops on either side of an enzyme...using this sensor to detect HMOs found on cellular surfaces. After incubating the sensor with bacteria engineered...

  33. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...sensors using self-associating fluorescent domains. ACS Chem Biol. 2013 Oct 18;8(10):2133-9. doi: 10.1021...field. Those engineered sensors were designed to replace synthetic calcium dyes, which had been in use since...particular advantage for observing events taking place inside cells that are on the move, such as T lymphocytes...FRET-based sensor proteins, particularly for the intracellular imaging of transition metal ions, such as zinc...

  34. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ... you could obtain arrays A, B, C, AB, AC, BC, and ABC). Nielsen Lab E. coli CRMAGE Plasmids: The CRMAGE...Multiplex Transcriptional Repression in E. coli.” ACS Synthetic Biology 4(9) (2015):987-1000. PubMed PMID...nontargeting spacer flanked by two CRISPR repeats. The spacer can be digested using BsaI, allowing a spacer-repeat...using 3 distinct spacer-repeats, you could easily create 7 unique arrays (e.g. for spacers A, B, and C, you...promoter-gRNA construct, but with different sequences adjacent to the sites. When digested with the appropriate...plasmids are digested, unique overhangs (here, O1-4) adjacent to the cut sites “link” fragments together and...

  36. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...in more contexts. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR...developed the Phage-Assisted Continuous Evolution (PACE) and Phage-Assisted Non-Continuous Evolution (PANCE...mutations, selection, and replications per day.  The PACE and PANCE systems use M13 bacteriophage, which rely...dilution step represents the major difference between PACE and PANCE: for PANCE the phage progeny is not diluted...-stringency PANCE, followed by higher stringency PACE, resulted in the evolution of TadA variants with...

  38. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ... modular assembly of broad-host-range plasmids." ACS synthetic biology 7.5 (2018): 1279-1290. PubMed PMID...

  39. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. ...

  40. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...Streptomyces Species Using an Engineered CRISPR/Cas System. ACS Synth Biol 4:723–728 . https://doi.org/10.1021/sb500351f...for Modular Assembly of Broad-Host-Range Plasmids. ACS Synth Biol 7:1279–1290 . https://doi.org/10.1021/... most CRISPR-based genome engineering has taken place in other organisms. In many bacteria, unlike other...and chloramphenicol resistance pCRISPR: carries a spacer targeting the gene of interest and kanamycin resistance...template, and the locus cannot be recognized by the spacer-derived crRNA. However, if recombineering is unsuccessful...survive have inactivating mutations in the Cas9 or spacer sequence, and these rare events are easily detectable...successfully in Tatumella citrea, another species of Enterobacteriaceae, without the need for modification. This ...

  41. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...provided by the Rinehart lab. Steinfeld et al., ACS Chem Biol 2014 May 16;9(5):1104-12. Heinemann et... your own designer bacteria. St-Pierre et al., ACS Synth Biol 2013 Sep 20;2(9):537-41. DREADD-based... One. 2011. Feb 18;6(2):e16765. Engler et al., ACS Synth Biol 2014. Feb 5 (Web); DOI: 10.1021/sb4001504...endogenous protein of interest and co-expressing its replacement recombinant protein with a FKBP domain and a ...tyrosine kinases (RTKs) are a large class of cell-surface receptors which play a critical role in development...dimerize on exposure to light, bringing the intracellular kinase domains into contact and initiating signaling...optimal scaffold can depend on the length of the spacer region between the target sequences. These updates...

  43. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...Wingler L, Pascolutti R, Paulo JA, Gygi SP, Kruse AC (2017) Multidimensional Tracking of GPCR Signaling...is initiated when a ligand binds to the extracellular surface of the GPCR. This results in a conformational...induce or inhibit intracellular signaling cascades as a response to the extracellular stimuli. Ligand dissociation...coupled with heterotrimeric G proteins on the intracellular side of the cell membrane. Heterotrimeric G ...relatively insensitive to their endogenous ligand, acetylcholine, and are only activated upon binding of CNO ...

  44. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ... the decaffeination plasmid here. Quandt et al. ACS Synth Biol. 2013. PubMed PMID: 23654268. FLINC...OptoSTIM1), caused two-fold faster changes in intracellular Ca2+ levels than the original version containing...substrate, inducing a conformational change that placesTagRFP-T and Dronpa in close proximity. By monitoring...

  45. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...821. doi:10.1126/science.1225829  Hazan J, Bester AC. CRISPR-Based Approaches for the High-Throughput ...with multiple gRNAs poses the risk of disrupting adjacent genes, especially those regulated by or overlapping...al. 2019). The interactions between ncRNAs and adjacent genes are complex and not yet fully understood...

  46. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...Receptors Exclusively Activated by Designer Drugs. ACS Chemical Neuroscience 6:476–484 . https://doi.org...nonnative ligands. However, they retain their intracellular interactions and still set off the same pathways...

  47. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...editing toolbox.  References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR...in the genomic DNA to contain a specific protospacer adjacent motif (PAM) made up of 5′-NGG-3′ (“N” can...eighth round of ABE evolution and the “x” is the placeholder for the number that identifies the mutation in...to ABE7.10. Depending on the position in the protospacer, the editing capacity of the ABE8s ranged from...To expand the targeting scope of ABE8, the team replaced S. pyogenes Cas9 in the ABE complex with PAM-variant...

  48. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...retain some ability to induce Cas9 to make edits (AC, WLC, and JQ, unpublished results). An additional...that is complementary to the target site, or protospacer, in the genome. Native Cas9 has two catalytic...which cleaves one strand of DNA upon binding the protospacer. The resulting double strand break (DSB) stimulates...

  50. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...References and resources References Bertiaux E, Balestra AC, Bournonville L, et al (2021) Expansion microscopy...?  ExM provides a straightforward answer to the space problem. Instead of imaging smaller, what if we ...
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