Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 444 results for: Ank

Showing: 1 - 50 of 444 results

  1. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...Fill out our guest blogger form to get started!   Thanks for all your hard work!   Fluorophores When is...Casilio is no longer available through Addgene.  Thanks again to all of our fantastic depositors and guest...

  3. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

    Type
    Blog Post
    Published
    March 10, 2015, 2:25 p.m.
    ...contributed by the Developmental Studies Hybridoma Bank. Antibodies are the most widely used class of protein-binding...immunoprecipitation and more. The Developmental Studies Hybridoma Bank (DSHB), directed by David R. Soll, is a repository...Development (NICHD) in 1986, to enable basic research by banking and distributing monoclonal antibodies at cost....quality-control and technical support to the community. Thanks to Our Guest Blogger!   David Soll has been the... Director of the Developmental Studies Hybridoma Bank (DSHB) since 1996.       More Blog Posts about Scientific...

  5. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    Published
    Oct. 10, 2019, 12:41 p.m.
    ...fused to a fluorescent protein. “Frankenbody,” like the infamous frankenstein, was constructed by grafting...called this probe the HA frankenbody (Zhao et al., 2019).  To develop HA frankenbody, the Stasevich lab tested...can you do with the HA frankenbody? Another large advantage of the HA frankenbody is that it remains bound...provides HA frankenbody the ability to bind nascent HA tagged proteins.  Lastly, the HA frankenbody can be ...properly once inside cells.  Developing the HA frankenbody To combat the issues with these antibody based...lab observed colocalization of GFP tagged HA frankenbody with an mCherry, 4xHA tagged histone protein ... in U2OS cells. They then showed that the HA frankenbody probe could also bind a 1xHA tagged histone protein...

  6. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...-HA frankenbodies include mEGFP, mCherry, and HaloTag. Figure 1: (a) Anti-FLAG frankenbody (FB)...Here's what you'll find in this post: Anti-FLAG Frankenbodies for modular imaging of cellular targets Optimized...mammalian genome regulation and editing Anti-FLAG Frankenbodies for modular imaging of cellular targets by: ...: Mike Lacy Adding to their set of anti-HA frankenbodies (Zhao et al. 2019), Tim Stasevich and Hiroshi... have recently published a set of anti-FLAG frankenbodies, a flexible, modular tool for labeling. Here...variety of applications. This set of new anti-FLAG frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and...Liu et al. 2021. Anti-FLAG and anti-HA frankenbodies have a variety of applications, including live-cell...

  7. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...the phage integrates into the bacterial genome flanked by two new recombination sites (attL-left- and ...BP Reaction takes place between the attB sites flanking the insert and the attP sites of the donor vector...the entry clone containing the DNA of interest flanked by attL sites. As a byproduct of the reaction, ... LR reactions. The BP reaction creates an attL-flanked entry clone. The LR reaction creates an expression..., an expression clone with the DNA of interest flanked by attB sites is generated. As in the BP reaction... generate our desired entry clone - human KRAS flanked by attL sites.  Method A: recombination of an attB-PCR...create an entry clone: recombination of an attB-flanked PCR product with an attP-containing donor vector...

  8. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ...summary per batch. Ranking guides for HDR in-depth In other tools, guides are ranked by specificity of ...genome by eye. We came up with a ranking formula that does just this: Rank = (compressed CFD score) * (gaussian...employs biological best practices. For example, it ranks guides by CFD score, and it mutates HDR donor templates...between 0 and 1) so that they can be used in the rank equation above. Cut-to-insert distance was fitted...in the graph below) so that it can be used in the rank equation above. Generally, the greater this distance...chanzuckerberg.com. We're eager to hear your thoughts. Many thanks to our guest blogger, Greg Dingle! Greg Dingle ...

  9. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...innovation between legal and scientific fields.” Thanks to our legal team, and great customer service, ...and our visibility is increasing internationally, thanks to evolving outreach tactics, increasing business... have a UI/UX team. Last time I checked we were ranked as the 21,806th most popular website in the world...Directors is mature and poised to support our growth. Thanks to Joanna Brownstein (our outgoing Chair) and Dave... incorporate additional Board best practices. I thank them wholeheartedly for their service and support...excellent intentions, or incredible drive to succeed.  Thanks to all the Addgenies and congratulations to current...current and alumni Addgenies on our success. Thanks to our amazing team, we also don’t have to worry about...

  10. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...outside of their recognition sequence, creating flanking overhangs. These overhangs can be customized to...vectors each contain Type IIS restriction sites flanking the promoter-gRNA construct, but with different...with the appropriate Type IIS enzyme, the unique flanking overhang sequences can link together to allow ...plasmids contain Type IIS restriction sites that flank the promoter-gRNA constructs. When these plasmids...promoters, the gRNAs are transcribed together and are flanked by specific sites that allow them to be cleaved...nuclease system to cleave the gRNAs. gRNAs are flanked by glycine tRNAs to create polycistronic glycine...CRISPathBrick plasmid contains a nontargeting spacer flanked by two CRISPR repeats. The spacer can be digested...

  11. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...in brackets and add the upstream and downstream flanking sequences to their input string (Figure 2). This...provides users with different designs of pegRNAs, ranked by their PRIDICT/PRIDICT2.0 score. For the best...editing strategies) based on a DeepSpCas9 score ranking (Kim et al., 2019). PRIDICT saves time and resources... Nicolas Mathis received his PhD from Gerald Schwank's lab at the University of Zurich (Switzerland),...L., Solari, C., Balázs, Z., Krauthammer, M., & Schwank, G. (2023). Predicting prime editing efficiency...Böck, D., Steensel, B. van, Krauthammer, M., & Schwank, G. (2024). Machine learning prediction of prime...

  12. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...copies the sequence from a repair template with flanking sequence homology for error-free DSB repair. NHEJ...MMEJ uses regions with 5-25 bp of microhomology flanking a DSB to repair DNA. The DNA ends are chewed back...figure above, short (5-25 bp) regions of homology flanking a double-stranded break yield precise deletion... is likely to introduce additional nucleotides flanking the insertion. If the DNA ends anneal incorrectly...5’ and 3’ microhomology to the insertion locus flanking the GFP-Puro cassette. Three double stranded breaks...The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and 3' microhomology and PITCh-gRNA binding...

  13. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...reversible. Whereas the excision of a piece of DNA flanked by loxP/FRT sites is favored over its reintroduction...vectors, the DNA sequence that should be inverted is flanked by two pairs of target sites that differ in their...with identical sequence leads to inversion of the flanked DNA sequence and the interior target sites. In ... leaving behind a vector with the inverted DNA flanked by non-compatible target sites (Schnütgen et al...genes in specific cells at specific times. Many thanks to our guest blogger, Katrin Michel! Katrin Michel.... PubMed Central PMCID: PMC41457. 8. Schnütgen, Frank et al. 2003. “A Directional Strategy for Monitoring...

  14. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...the blog. Thank you! More resources on the Addgene blog 10 Great Guest Posts We're Thankful For Lucky...It’s a very thankful time of year for those of us at Addgene! And we can’t think of any better way to...to start off our holiday season than by thanking the twelve guest bloggers and subject matter experts ...experts who have contributed to our blog in 2024. Thank you to…   Marty LaFleur, who shared information about...

  15. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...service is successfully launched, I would like to thank some of the people and organizations who helped ...milestone. Viral service supporters We would like to thank the companies who supported this project with funding...viral preps. Visit our Sponsors and Supporters Page Thank you to all of our generous, sharing depositors. ...encouraging us to explore new directions. I would like to thank them for their time, dedication, advice and encouragement...this well ... because it did. So finally, a big thank you to the Addgenies who went the extra mile to ...

  16. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...tool that responds strongly to magnetic fields (Frank Hayward et al., 2024). They deposited their plasmid...when a small magnet was passed over the sample (Frank Hayward et al., 2024). The fluorescence intensity...the magnetic field is on. Image reproduced from Frank Hayward et al. 2024 under CC-BY license. They...the magnetic field is on. Image reproduced from Frank Hayward et al. 2024 under CC-BY license.   ...468. https://doi.org/10.1016/j.bpj.2019.01.003.  Frank Hayward, R., Rai, A., Lazzari-Dean, J. R., Lefebvre...

  17. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ... Addgenies use our social media and blog to say thank you to all the many scientists and researchers who...12,427 plasmids deposited with us. We cannot say thank you enough - but this week we’re certainly going...   Here’s our Depositor Week schedule:  Monday: Thank you post (this is it!)  Tuesday: Stats roundup on...Addgene swag.  And, of course, to all our depositors: thank you so much!!!  (A special shout-out to those who...

  18. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...antibodies (mAbs). mAbs have been around since 1975 thanks to the Nobel prize winning work of Milstein and...single molecule tracking in living cells. The HA frankenbody is an HA tag detection probe. It contains an ...to a fluorescent protein such as GFP. The HA frankenbody works just like an antibody-based probe, but ... resources on the Addgene blog: Read about frankenbodies, an anti-HA scFv-based probe for fluorescent... antibodies, the Developmental Studies Hybridoma Bank Learn more about the SunTag system which uses an...

  19. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    Published
    Feb. 9, 2017, 3:30 p.m.
    ...the interview with Tom McFadden!   1. Rosalind Franklin vs. Watson & Crick - Science History Rap Battle...telling following the unfortunate story of Rosalind Franklin and her incredibly important role in solving the...Fill in the Planck at sciencewithtom.com. Many thanks to Tom McFadden for taking the time to sit down...youtube channel and at sciencewithtom.com.   Special thanks to our sound engineer/producer, Eddy Page! Edward...

  20. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    Published
    July 7, 2017, 1:32 p.m.
    ...without exclusivity. Chapter 11: The Stem Cell Bank (CReM Bank) at the Center for Regenerative Medicine and...did I start doing this in the first place?" Many thanks to our guest, Darrell Kotton. Dr. Kotton is the...Boston University and Boston Medical Center. Special thanks to our sound engineer/producer, Eddy Page! Edward...

  21. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ... 17] should cover it. Addgene: OK, thanks for your time, and thanks for depositing your plasmids with ...Howarth: Just the usual blood, sweat and tears. (Thanks to our healthy blood donor for keeping going while...be of use to others to Addgene. I am a big fan; thanks for the T-shirt! References: SpyLigase peptide-peptide...

  22. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...package. In a transfer plasmid, the transgene is flanked by two ITRs, and the ITRs are retained with the...Figure 1: Recombinant AAV vector with ITRs that flank the gene cassette, which contains a promoter, the...ITRs are symmetrical 145-nucleotide sequences that flank the ends of the single stranded DNA genome of AAV...the native virus. In an AAV vector plasmid, they flank the gene of interest. A single ITR consists of palindromic...

  23. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...results: On this page you can download an annotated GenBank File or a SnapGene file for the sequences listed.... If the depositor provided a custom annotated GenBank file, this can be found on the main plasmid page...relevant), and types. Types listed are standard GenBank feature types. Clicking on a particular feature...your next experiment. We would like to extend our thanks to you (our community of scientists) for suggesting...

  24. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...to have 50–80 bp of homology arms on each side flanking the change you'd like to introduce. We use Ultramer...plasmid donor, with two homology arms on each side flanking your desired insertion or mutation. Each arm is...pX330/PX335 system. Additionally, I would like to thank Le Cong for all his hard work creating amazing resources...community and sharing these resources with Addgene. Thank you, Le! This blog post was updated to include all...

  25. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...Harvard Medical School. We want to give a special thanks to the early-adopter scientists, who trusted Addgene...to hear the stories others have to tell.  "Also thanks for being Addgene. You guys rock." — Andrew York...Calico Life Sciences. Most of all, we’re incredibly thankful for all the scientists who have joined our community...your work, your research, and your discoveries. Thank you. You guys rock. ...

  26. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    Published
    Feb. 28, 2019, 1:34 p.m.
    ...reproducibility and shared similar desires to take action. Thanks to Teytelman’s vast network and ability to identify...thinking about ways to address irreproducibility and thanks to Teytelman’s connection with Joanne Kamens, Addgene... them to connect? Through networking of course! Thanks to individuals in the group who were well connected...collaborate with this incredibly dedicated team. Many thanks to Tyler Ford, Caroline LaManna, Joanne Kamens,...

  27. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...didn't have the space to give proper credit to. Thankfully, as Addgene's resident blog editor, I could take...can refold correctly after denaturing.  Designed ankyrin repeat proteins, or DARPins, are genetically engineered...derived from a class of binding proteins known as ankyrin repeat proteins. DARPins typically consist of 3...an excellent option for many applications, and frankly, if ain't broke, don't fix it!  That being said...

  28. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...the design of flanking overhangs. Although there are theoretically 256 distinct flanking sequences, sequences...their recognition sequence, creating four base flanking overhangs. Since these overhangs are not part ...can engineer unique enzyme recognition sites that flank their DNA fragment in an inverse orientation. This...

  29. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...recombination events, and that the orientation of the flanking loxP sites directs the Cre recombinase to invert...while FLP binds FRT sites. When a DNA sequence is flanked by target sites (floxed) in opposing orientations...Figure 1, top). The entire DNA cassette would be flanked by two pairs of target sites- one wild-type pair...placement of the two pairs of target sites. By flanking the reporter with one loxP site and one lox511...

  30. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...vector contains a neomycin resistance gene (NeoR) flanked by homology arms. The negative selection marker...” the resistance gene through the insertion of flanking LoxP sequences, you can remove the gene through...targeting vector such that a set of three LoxP sites flank the resistance gene and the targeted exon in the...has simply been replaced with the same sequence flanked by LoxP sites while the resistance cassette has...

  31. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...he describes the now infamous tale of Rosalind Franklin and her essential-but-unlauded role in the identification...within this section. The GFP story has its own Franklin in Doug Prasher, who originally isolated the GFP...science produced by the quieter, more reserved Franklins and Prashers will find it increasingly difficult...your career.  Acknowledgements: I would like to thank fellow Addgenies Joanne Kamens, Marcy Patrick, Aliyah...

  32. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...two sets of sgRNAs; A, the top 3 ranked sgRNAs, and B, the next 3 ranked sgRNAs. Dolcetto was able to discriminate... lab selected sgRNAs in this optimal window and ranked them based on their optimized sgRNA rules and potential... and Dolcetto library, sgRNAs were selected and ranked based on the previously established sgRNA rules...

  33. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...requested through Addgene. Their plasmid maps and GenBank files can be found on their plasmid pages. All ...terminators, promoters, and genes) were built with flanking primer binding sites (designed to have 20-30 nt...parts are also annotated on the plasmid maps and GenBank files uploaded to Addgene. We also recommend inserting...characterization of the recombinases at our disposal.  Many thanks to our guest blogger Nathaniel Roquet! Nathaniel...

  34. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...primers that amplify the DNA sequence of interest and flank it with 50 base pairs of homology to the targeted...interest and 50 nucleotides of homology to regions flanking the target site).  The best applications for dsDNA...limited circumstances. An alternative approach is to flank the desired change with 4-5 silent changes in the...recombineering system? Share your tips below! Many thanks to our guest blogger, Beth Kenkel Beth Kenkel is...

  35. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...Hershey-Chase experiment. In the same year, Rosalind Franklin and her student Raymond Gosling took the first...diffracted image of the DNA. Finally, in 1953, Franklin along with James Watson and Francis Crick resolved...Inferno (2013) and the movie version (2016) stars Tom Hanks as the protagonist (Langdon). The plot involves ...?Download high resolution versions here.  Many thanks to our guest blogger Kartik Lakshmi Rallapalli ...

  36. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ...cable ties) received a festive makeover today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo...uDEE9PsspD#deckthelab #virussnowflakes #sciart #thankyouscience #scicomm pic.twitter.com/DWKWaPs0iY— Xi Chen...  Congratulations to all our winners, and a huge thank you to everyone who decked their lab and brought...

  37. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...importance of sharing information/knowledge Outro Many thanks to our guests, Melina Fan, Kenneth Fan, and Benjie...Officer. Prior to Addgene, Kenneth was an investment banking analyst at Credit Suisse’s Technology Group in ...Engineering from MIT, both in Computer Science.   Special thanks to our sound engineer/producer, Eddy Page! Edward...

  38. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...Addgene to succeed and to improve, so thank you. 500,000x Thank You! Addgene's new location: 75 Sidney...for our favorite people – you, our scientists!  Thank you for being part of our growing community! Our...

  39. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...accessible for scientists as possible.  He also thanked our many business partners, without whom Addgene...essential advice and service. Joanne shared a moving thank you email from a requestor in Iraq (Addgene's first...family, too. Here’s to another great 10 years!    Thank you to our guest blogger! Blugene, Addgene's friendly...

  40. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ... genuine pleasure to send these small tokens of thanks for the incredible impact our depositors have had...particular our Blue Flame Award winners- we’d like to thank you for sharing your plasmids at Addgene. You are... we’re able to do what we do!          Special Thanks Trophies and engraving services from Hit Trophy...

  41. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ... of HEK cells with adenovirus 5 DNA was postdoc Frank Graham’s 293rd experiment, hence the nomenclature...endothelial, epithelial, or fibroblast. However, Frank Graham showed HEKs display many immature neuron-...great model of kidney cell biology, there are thankfully many other applications that they are useful ...

  42. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ... getting these reagents is profound.” Frank Stegmeier, PhD Frank Stegmeier is the Chief Scientific Officer...members to the Board of Directors: Khandan Baradaran, Frank Stegmeier, and Luk Vandenberghe. We’re excited to...

  43. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...control. For easier portability, all viral genes are flanked by EcoRI and BamHI restriction sites - so cloning...to mention that our FedEx guy has been the best: Thanks, Todd! As you can imagine, it’s also been quite...related plasmids and resources at Addgene* Many thanks to our guest bloggers Manon Eckhardt (left) and...

  44. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...of the De-jargonizer.   Academic Phrasebank  The Academic Phrasebank is a resource for phrases commonly...being critical, or defining terms. The phrases in Phrasebank are generic, so they need to be edited to suit...

  45. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...s flattering to ask.  Show appreciation. Send a thank you note or gift to someone who helped you succeed...any way. Don’t believe people who tell you that thank you isn’t necessary. I once got a box of 6 perfect...helped someone get a job interview. Even just a thank you email would have been enough, but I’ll never...

  46. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...reference protein) under identical conditions, a ranking of the practical brightness can be made (Goedhart...repeating this with another fluorescent protein, a ranking of practical brightness can be made. This strategy...different emission filters) or different set-ups. Many thanks to our guest bloggers Joachim Goedhard and Marieke...

  47. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...heterogeneous when constrained by sequence identities in flanking sequence (“microhomologies”). NHEJ doesn’t obligatorily...canonical Cas9 approach. A pair of CRISPR guides that flank regions of hundreds of base pairs or more can simultaneously...Christina Mork contributed to the update of this post. Thank you to our guest bloggers!   David Wyatt is a graduate...

  48. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...the Max Planck Institute for Brain Research in Frankfurt am Main, Germany. She presented a novel technology...to be invited to learn from them at the MPI in Frankfurt am Main and perform similar experiments. Visualizing...endogenous proteins in any cell type. Exciting! Many thanks to our guest blogger, Eugenia Rojas! Eugenia Rojas...

  49. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...several human and murine CRISPR pooled libraries, but thanks to a deposit from the laboratory of Dr. Ji-Long...constructs, and complete screening in just 3 days? Thanks to Tyler Jacks’ lab, this 3-day goal is is now ...vectors contain uracil-excision based (USER) sites, flanked by ADH1 and CYC terminators for easy cloning of...revolutionized genome editing in multiple organisms thanks to its simple, easily modifiable RNA-guided targeting...difficult to quantify interaction strengths. Erich Wanker’s group at the Max-Delbrueck Center for Molecular...

  50. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ... the interview. Greet each interviewer and then thank them once the interview is complete. Remember, your...you, so be engaged with their conversation. Send thank you emails shortly after the interview to the hiring...took during your interviews to help craft these thank you's. Interviewing remotely Remote interviews are...
Showing: 1 - 50 of 444 results