We narrowed to 86 results for: CAST

...called evoCAST — an evolved CRISPR-associated transposase (CAST). Back up a second. What are CASTs? CASTs...V-K CAST systems use a nuclease-deficient Cas12k (Gelsinger et al., 2024). In either type of CAST, the...only CAST integration of the promoter could turn the gene on. Once phage success was linked to CAST efficiency...isolated these more efficient CASTs (Witte et al., 2025). The best evoCAST featured 20 mutations in TnsB... bacterial genomes (Peters et al., 2017). Type I CAST systems rely on the Cascade complex minus its nuclease...even entire genes. Figure 1: Mechanism of CAST systems. A Cas effector (Cascade in Type I systems...gRNA-specified sites. Created with BioRender.com. CASTs quickly became popular bacterial genome engineering...

... In this episode of the Addgene podcast, we sit down with Addgene’s Founders - Melina Fan, Ken Fan, and...Listen to the interview with Addgene's founders Podcast breakdown Intro Chapter 1 - Who are Addgene's Founders...the audio engineer and producer for the Addgene Podcast.      Additional Resources from the Addgene Blog... podcast...

... the Addgene Podcast.     Additional Resources on the Addgene Blog Listen to Our Podcast Interview with...plasmid info with a new segment on the Addgene podcast - The Hot Plasmids Segment. Click on the player...below to listen to a quick (~5 min) Hot Plasmids podcast that introduces you to 4 new plasmid technologies... the latest and greatest plasmids through this podcast segment. Below, we break down each of the Hot Plasmids...If you have any comments about the Hot Plasmids Podcast Segment, or have recommendations for new Hot Plasmids... podcast...

...scientific collaboration across the globe. Listen here! Podcast Breakdown (Time: Topic) 0:00-1:00 : Intro 1:01-...the audio engineer and producer for the Addgene Podcast.     Additional Resources Learn about Seeding ...the Addgene Blog Listen to Additional Addgene Podcasts Learn How You Can Contribute to the Addgene Blog...

... the Addgene Podcast.     Additional Resources on the Addgene Blog Listen to Our Podcast Interview with...In the second episode of our Hot Plasmids podcast series, you'll learn about new red fluorescent proteins...plasmids webpage. Listen to the entire Hot Plasmids podcast! Hot plasmids covered in this segment Hot Plasmid...

... the Addgene Podcast.     Additional Resources on the Addgene Blog Listen to Our Podcast Interview with...In the third episode of our Hot Plasmids podcast series, you'll learn about optogenetics tools for controlling...

...the Dark Lord rules all, the books center around a cast of endearing characters who decide to take matters...Ice Age to the 1980s, Sarum follows an intriguing cast of characters from five different families over ...

... were absolutely abuzz with anticipation as they cast their ballots for their favorite submission. So ...

...often emerges at the intersections of disciplines. Cast your net wider, explore tangential subjects, and...

...experimental fit due to this. But now, it’s time to cast aside those concerns and embrace a new resource ...

...-Assisted Targeting) by the Sternberg group, and CAST (CRISPR-associated Transposase) by the Zhang group...

...air bubbles or high concentration of fluorophores) cast shadows along the illumination path. The shadows...

... called V-K, is a CRISPR-associated transposase (CAST) that can insert large fragments of DNA. The V-F...

...RNA-Assisted Targeting) and the Zhang lab's system called CAST (CRISPR-associated Transposase) both enable gRNA-directed...

...single-percentage gel, you will have the option to cast your own gel. Handcast gels are significantly cheaper and generate...Tris-glycine 100 V, 1–2 hours Easy and cheap to handcast Short shelf-life; can alter proteins due to ...250 kDa Different sizes 4–20% gradient   Handcast or premade If you need a gradient gel, the decision...already made: gradients are extremely difficult to handcast, so unless you have an expert around, you’ll likely...hazardous chemicals, and are not always consistent. Precast gels, while expensive, reduce protocol time, can... CCD camera High initial (purchase) cost Precast gel Increased cost Save money Polyclonal... quickly Wet transfer Increased time Handcast gel Increased time; have to make as-needed ...

...Pint series. Chapter 4: The SITN Podcast, Sit'N Listen - In their podcast, the SITN team brings in scientists...The Goal of the SITN Podcast - Vini and Amy would like to develop the SITN podcast as a means for listeners...senior.  To learn more about the STIN's podcast, email sitnpodcast@gmail.com    For more info about starting...have to be crazy complicated! Listen to the full podcast for more great science communication tips or listen...of Science in the News, and member of the SITN podcast team. You can follow Vini on twitter @vinidhra....Science in the News, and is a member of the SITN podcast team. You can follow Amy on twitter @aigilson. ...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ...

...Listen to our hot plasmids segment on the Addgene Podcast. Subscribe to the Addgene blog to get all the ... Listen to Our Tol2 Gateway-Compatible Toolbox Podcast Segment The impact of KLF4 N-terminal variants ...Neuroscience. 2016. PubMed PMID: 27798629 Listen to Our Podcast Segment on This New AAV Tool    mCyRFP1: Novel.... PubMed PMID: 27798609 Listen to our mCyRFP1 Podcast Segment New bright monomeric red fluorescent protein... PubMed PMID: 27869816 Listen to our mScarlet Podcast Segment    Photocleavable protein PhoCl expands...Methods. 2017. PubMed PMID: 28288123 Listen to Our Podcast Segment on PhoCl LSSmCherry1 & RDSmCherry1: Engineering...Methods. 2017. PubMed PMID: 28417998 Listen to Our Podcast Segment on Coselection with Ouabain New CRISPR ...

...science communication. Science in the News hosts a podcast, seminars, a blog, conferences, and more for non-scientists...the interview with Science in the News, Part 1 Podcast breakdown Introduction to Vini, Amy, and SITN ...Approach Different Audiences Listen to the SITN Podcast Many thanks to our guests, Vini Mani and Amy Gilson...of Science in the News, and member of the SITN podcast team.     Amy Gilson recently received her PhD ...Science in the News, and is a member of the SITN podcast team. You can follow Amy on twitter @aigilson. ...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ...

...In today’s podcast, we sit down with Wei Leong Chew, a researcher at the Genome Institute of Singapore... Listen to the interview with Wei Leong Chew! Podcast breakdown Intro: Wei Leong Chew's career path and...the audio engineer and producer for the Addgene Podcast.      Additional Resources from the Addgene Blog...featured topic page Check out our Hot Plasmids Podcast Listen to our interview with synthetic biology ... addgene podcast...

... Listen here! Podcast breakdown (Time : Topic) 0:00 - 2:00 : Introduction to the podcast from producers...In this episode of the Addgene podcast, we sit down with Alex Chavez, a Clinical Fellow in Pathology ...Engineering. Alex is the first MD PhD we've had on the podcast, has worked extensively with CRISPR, and recently...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ...