...into the genome. CRISPR-associated transposases ( CASTs , developed by the Feng Zhang lab ) combine the ...have also been developed. The two main classes of CAST systems are type I and type V-K. Type I systems ...carries out integration of donor DNA. Both types of CASTs have been found to achieve donor DNA integration...further improvements are actively in progress. Type I CASTs can result in bidirectional insertions — meaning...orientation of the insert is important. Type V-K CASTs appear to exhibit almost unidirectional insertions... (12), 770–788. PMID: 30323312 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S....