... how to perform gel electrophoresis. Equipment Casting tray Well combs Voltage source Gel box UV light...

...chamber. Use the metal spatula to gently break the gel cast open. Use a razor blade to cut the top of the wells...

...protein concentration in the lysate, running a precast SDS-PAGE gel, and immunoblotting....protein concentration in the lysate, running a precast SDS-PAGE gel, and immunoblotting. Sharing speeds...23227 β-mercaptoethanol 4X protein loading buffer Precast SDS-PAGE gel SDS-PAGE running buffer Prestained...min at 100 °C . Section 3: SDS-PAGE Prepare the precast gel as follows: Remove the gel from the plastic...