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  1. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ... CRISPR CASTs CRISPR Plasmids: CRISPR Transposases (CASTs) Browse...transposases (CASTs) for insertion of donor DNA. CRISPR...CRISPR Blog Posts CRISPR-associated transposases (CASTs) combine the large-scale capacity of transposases...transposase can then integrate donor DNA. Editing by CASTs is not considered "scarless", as the transposon ...along with the donor DNA. The two main classes of CASTs are type I and type V-K. Type I systems use a type...components (TnsA, TnsB, TnsC, and TniQ). Type I CASTs can result in bidirectional insertions and may not...(TnsB, TnsC, and TniQ). Unlike type I, type V-K CASTs appear to exhibit almost unidirectional insertions...

  2. CRISPR Guide

    Type
    Collection
    ...into the genome. CRISPR-associated transposases ( CASTs , developed by the Feng Zhang lab ) combine the ...have also been developed. The two main classes of CAST systems are type I and type V-K. Type I systems ...carries out integration of donor DNA. Both types of CASTs have been found to achieve donor DNA integration...further improvements are actively in progress. Type I CASTs can result in bidirectional insertions — meaning...orientation of the insert is important. Type V-K CASTs appear to exhibit almost unidirectional insertions... (12), 770–788. PMID: 30323312 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S....

  3. Plant Plasmids and Resources

    Type
    Collection
    ...development. The TapRoot - Podcast (Link opens in a new window) - The Taproot is a podcast to understand how scientific...literature are discussed with one of the authors. The podcast is produced by Plantae (Link opens in a new window...

  5. Cre-lox system

    Type
    Collection
    ...gTc’v; Tc-hsp68:Cre) Cre Tribolium casteneum hsp68 T. castaneum (red flour beetle) Averof 119868 pGEM-NLS-Cre...

  6. Rett Syndrome

    Type
    Collection
    ...Knock-in of NLuc-tdTomato at endogenous MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (...

  7. The Pleiades Promoter Project

    Type
    Collection
    ...Black SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen Y, Cheng JC, Chopra V, Docking...
Showing: 1 - 8 of 8 results