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We narrowed to 7 results for: CAST

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Showing: 1 - 7 of 7 results

  1. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ... CRISPR CASTs CRISPR Plasmids: CRISPR Transposases (CASTs) Mammalian...transposases (CASTs) for insertion of donor DNA. CRISPR...Bacteria Resources CRISPR-associated transposases (CASTs) combine the large-scale capacity of transposases...transposase can then integrate donor DNA. Editing by CASTs is not considered "scarless", as the transposon ...along with the donor DNA. The two main classes of CASTs are type I and type V-K. Type I systems use a type...components (TnsA, TnsB, TnsC, and TniQ). Some type I CASTs can result in bidirectional insertions — meaning...orientation of the insert is important. However, type I-F CASTs favor insertion in the T-RL direction, with the ...

  2. CRISPR Guide

    Type
    Collection
    ...into the genome. CRISPR-associated transposases ( CASTs , developed by the Feng Zhang lab ) combine the ...have also been developed. The two main classes of CAST systems are type I and type V-K. Type I systems ...carries out integration of donor DNA. Both types of CASTs have been found to achieve donor DNA integration...improvements are actively in progress. Some type I CASTs can result in bidirectional insertions — meaning...orientation of the insert is important. However, type I-F CASTs favor insertion in the T-RL direction, with the ...homology end closest to the target site. Type V-K CASTs appear to exhibit almost unidirectional insertions...s41576-018-0059-1 PMID: 30323312 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S....

  3. Plant Plasmids and Resources

    Type
    Collection
    ...grasses. The TapRoot Podcast (Link opens in a new window) — The Taproot is a podcast to understand how scientific...literature are discussed with one of the authors. The podcast is produced by Plantae (Link opens in a new window...

  4. Rett Syndrome

    Type
    Collection
    ...Knock-in of NLuc-tdTomato at endogenous MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (...

  5. The Pleiades Promoter Project

    Type
    Collection
    ...Bonaguro, R. J., Brauer, E., Candido, T. R., Castellarin, M., Chen, J., Chen, Y., Cheng, J. C., Chopra...

  6. Tetracycline Inducible Expression

    Type
    Collection
    ...Dox-inducible expression of Cre recombinase. Madeline Lancaster 198752 DiLiCre 2.0 Dox-inducible expression of...
Showing: 1 - 7 of 7 results