We narrowed to 740 results for: NSI

... as one of the the skills favored by research-intensive careers. External collaborations are becoming ...important in a non-research context, especially when considering small to mid-sized companies and organizations... Addgene as a nonprofit organization is very considerate and thoughtful about its limited resources. Rather...find this to be the most challenging and time-intensive part of relationship building. However, it is ...without directly contacting them. One great way to transition from an internal to an external connection is...organization is important for success in research-intensive careers. In addition, this skill is a hallmark...Collaborate: An Imperative for Graduate Students" from Inside Higher Ed Additional Resources on the Addgene Blog...

...such as mice, which are less prolific and more expensive per individual to maintain. Also in contrast to...heart defects and cancer. These mutants are used extensively to study development and disease. Additional ...implemented in zebrafish. Developmental biology insights using lineage tracing in zebrafish To highlight...lineage tracing approaches that led to important insights in developmental biology. Microscopy-based lineage...embryo, where similar processes occur over days inside the mother, the zebrafish is an ideal system to...their long three-month generation time and labor-intensive maintenance compared to invertebrate model systems...highlight how these advantages can translate into insights in developmental biology, and advances in this...

...library/produced your lentivirus. Since viruses are sensitive to freeze/thaw cycles, we include a 1.3 mL aliquot... number of cells to transduce, you may want to consider spinfection. Spinfection is the process of transducing...transducing cells at a concentrated seeding density while centrifuging. Spinfection transduction efficiency...optimization of many variables, such as seeding density and reagent concentration.  Screening time Phew...place to ask experimental questions.  Practical Considerations for Using Pooled Lentiviral CRISPR Libraries...Libraries- A nice overview of what to consider when using pooled lentiviral CRISPR libraries. Protocols from... Waxmonsky NC, Swanson LE, Fan M. Practical Considerations for Using Pooled Lentiviral CRISPR Libraries...

...can be a time-intensive endeavor. That is why we also included an analogous set of transient constitutively-expressed...on the other hand, is a widely available and inexpensive imaging modality with good tissue penetration... be easily expressed heterologously by bacteria inside the body. So we screened gas vesicles gene clusters...bARGSer-expressing E. coli. The GV-specific ultrasound signal inside the tumors was overlaid with the anatomical B-mode...Emerald GFP. These plasmids can be used directly in transient transfection in rtTA+ cell lines (such as HEK293... using the most reliable method, which is the transient cotransfection of the mARG plamsids into HEK293T...

... the end of this post! Figure 1: Base transitions are edits between the purine bases (adenine, ...newly-deaminated inosines. Pro tip! The nucleoside inosine consists of the nucleobase hypoxanthine + a ribose sugar...transversion editor AYBEv3. This tool still retained considerable A → G editing activity at most sites, in addition... editor (gGBE) (Tong, Liu, et al., 2023). gGBE consists only of engineered MPG fused to nCas9. First, ...efficiency, purity, and indel rate of each tool vary considerably. And there’s so much more exciting protein engineering...is which. Acronym Full name Notes   Base transition Base change between purines (A ↔ G) or between... glycosylase to a CBE; the first type of base transition editor invented.   Abasic site A DNA site with...

...” in quotations here because I would not have considered myself neutral in any of their disagreements....Skills series, this post is not meant to be a comprehensive guide to Conflict Resolution. There are countless..... Ownership: Misunderstandings about who is responsible for what tasks or projects is a common source...customer support element amongst their other responsibilities. Knowing which aspects of customer support... require a manager to explicitly assign the responsibility in question to a specific person or team. ... more of a winner and loser–one party will be considered in the right, and one in the wrong. If you arbitrate...also aggravate long-term personal conflicts.  Transitioning a conflict into a collaboration may not be the...

...primary antibodies is a time consuming and expensive process. Consider the vast number of possible primary antibodies...we’ll go through some of the most important considerations to make when planning and performing an IF ...indirect immunofluorescence procedure with key considerations about each step. Image created with BioRender.com...’ll start there. Some of the most important considerations are to make sure your secondary 1) will recognize...remember that antibodies recognize very specific 3-dimensional epitopes. Different sample processing steps can...protocols available for each antibody that you consider in order to determine how reliable the antibody...

... imaging NIR FPs, consider using light sources that produce enough light intensities in NIR. Usually, ...supplied (Baloban et al., 2017).  It is important to consider effective brightness of NIR FPs in cells, in addition...Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs were developed...dDetermined as effective NIR fluorescence in transiently transfected live HeLa cells with no supply of...Designing brighter near-infrared fluorescent proteins: insights from structural and biochemical studies. Chem ... (2015). Piatkevich, K.D. et al. A robotic multidimensional directed evolution approach applied to fluorescent...

..., for some applications, integration might be considered a double-edged sword with unintended consequences...AKA transduction in the viral vector world). Once inside the host cell, the viral payload is integrated ...lentiviruses lack a functioning integrase, the enzyme responsible for inserting viral DNA into the host genome....genomes are transcriptionally active, leading to transient expression of gene products (Wu et al., 2004)....insertional mutagenesis (Howe et al., 2008). 2. Transient expression of transgenes/shRNAs If you want your...expressed (Fu et al., 2013). Using IDLVs ensures transient expression without the risks associated with prolonged...of clinical or basic research applications. Considerations for IDLV use If you’re reading this and thinking...

...results of cloning or knockout experiments to forensics and clinical diagnostics. One example of southern... a sample. Whereas Southern blotting is labor intensive and requires a large amount of high-quality DNA...lacking the 3’ hydroxyl group required for the extension of a DNA strand (8). Adding a small amount of ...of DNA strands of all possible lengths because extension is terminated upon the incorporation of a ddNTP...speed ultimately leading to what are generally considered first generation DNA sequencing machines (9).... drive for faster, higher throughput and less expensive sequencing fuels a sequencing revolution that ...1195397. 2. Chang, Judy C., and Yuet Wai Kan. "A sensitive new prenatal test for sickle-cell anemia." New...