We narrowed to 321 results for: bre
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TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....SRM), a family of imaging techniques developed to break through the resolution barrier while still utilizing...
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Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....is sufficient. Controls: Controls can make or break an experiment. The best controls for a colony PCR... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....Science. 1986; 231:699-704. PubMed PMID: 3080805. 3. Brent, R., and Ptashne, M. A eukaryotic transcriptional... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....facility). The third tube is used to fulfill orders. Breaking the (bar)code At each step in the receiving, transforming... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....small victory in a long road to your scientific breakthrough. More Reading: How to manage scientific data... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....studies, act as a reporter for DNA double strand break repair, and to label pathogenic intracellular microbes... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m.... insert encoded by the vector." The table below breaks down the biosafety concerns associated with various... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....exception is that men cannot actually give birth and breastfeed. Keeping this always at the forefront will go... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog PostPublishedNov. 19, 2020, 2:15 p.m....MECP2 gene, and highlights some of the latest breakthrough therapeutic approaches. What is Rett Syndrome... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog PostPublishedAug. 20, 2020, 2:30 p.m.... Cpf1), a nuclease that generates double strand breaks in target genes resulting in the formation of “... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog PostPublishedApril 16, 2020, 1 p.m.... generates 5’ and 3’ staggered ends after dsDNA breaks. The Doudna lab discovered Cas12a’s ability to ... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m....matching Mentors with Singletons for short coffee breaks. While this was not the more intense mentorship... -
An Inside Look into Addgene's Dev Team
TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m....collaborative approach to development. Scrum teams plan and break up work into small, well defined chunks, called ... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....single or multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added to the... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m.....12 (2007): 1079. PubMed PMID: 17700574. Hare, Brendan D., et al. "Optogenetic stimulation of medial prefrontal... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog PostPublishedAug. 13, 2019, 12:58 p.m.... genetic resources and highlight two recent breakthroughs in developmental biology that arose from zebrafish-based... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog PostPublishedMay 2, 2019, 12:41 p.m.... (Virginia Weis) Sea anemone Aiptasia - algae Breviolum minutum symbionts are important models for solutions... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog PostPublishedApril 2, 2019, 1:44 p.m....first conference room, that is actually used as a break room for Addgenies, was named after the protein ... -
CRISPR 101: Any Base Transversion Editors
TypeBlog PostPublishedApril 1, 2025, 1:15 p.m....also called apurinic or apyrimidinic sites and abbreviated as AP sites. A collaborative team from the David... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m..... abscessus. A) Sth1Cas9 mediates double strand break at the target site, which results in either bacterial... -
Antibodies 101: Reading a Flow Plot
TypeBlog PostPublishedFeb. 6, 2024, 3:13 p.m....first… which can send you in an endless loop. Let’s break out of the loop with all you need to know on how... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....incubation time, antibiotic conditions, and amount of polybrene. (psst…didn’t get your library pre-made from Addgene... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog PostPublishedJune 20, 2024, 1:15 p.m....strands. Created with BioRender.com. Once DnaA breaks the local hydrogen bonds holding the DNA together... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....Alcaligenes eutrophus, Azotobacter vinelandii, Brevibacterium methylicum, Caulobacter crescentus, Desulfovibrio... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog PostPublishedNov. 10, 2016, 3:30 p.m...., we can all work together to try and prevent outbreaks of drug resistant bacteria within communities,... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m....engineering single genome edits but the double strand breaks it introduces makes it difficult to perform two... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....it in the cleaved complex. This results in DNA breakage and cell death. Conversely, CcdA binds to and ... -
The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m....2002): 315-319. PubMed PMID: 12001993. 4. Lucey, Brendan P., Walter A. Nelson-Rees, and Grover M. Hutchins... -
Getting the Most from Your Lentiviral Transduction
TypeBlog PostPublishedApril 7, 2016, 2:30 p.m....typically employ the positively charged polycation, polybrene. Interestingly, one study found that alternative... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....behavioral effect but the function of specific subregions within the hippocampus were only recently studied...