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We narrowed to 920 results for: Gene

Showing: 361 - 400 of 920 results

  1. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...and topology, and cloning and gene expression. Just released: Addgene's Plasmids 101 (2nd Ed.) eBook... as everything from parasites, to symbionts, to genes and the labels applied to them were vague or contradictory...biology was never the same. Over the next few years genes from different bacterial (and eventually mammalian...Bioblasts? Plasmagenes? In the 1940s and 50s, scientists were working to understand genetic cytoplasmic...of "cytoplasm" and "id" (Latin for 'it'), as “a generic term for any extrachromsomal hereditary determinant...separate term, “episome”, defined as “a non-essential genetic element which could exist either autonomously or...molecular biologists wanting to study bacterial genetics. This all changed thanks, in part, to a collaboration...

  2. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...many of which are used to deliver Cas9 and other genes to mammalian cells. For this reason, the top requested...means researchers who use VSV-G can deliver their genes of interest to a variety of cells and study their...plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit. Who knows, maybe yours ... articles cite the use of pMD2.G obtained from Addgene. Skimming the list, one gets a sense of the broad...searchable list here). Tips for using pMD2.G At Addgene, we use pMD2.G for producing our ready-to-use lentivirus...Lentiviral Transduction Additional Resources on Addgene.org Check out our Viral Service Learn more about...

  3. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...on the Addgene blog Learn more about plasmid sharing during COVID-19 Resources on Addgene.org Find ...examine a dataset of Liu lab plasmid requests from Addgene, as a proxy for global activity in our particular...over 6 years for 35 papers (data was provided by Addgene on 5/26/20 and contains requests from 5/22/14 to...The inferred weekend effect is, in retrospect, generally more dramatic than the COVID-19 effect, though...not a causal interpretation -- the effect would generally not be the same if weekends were somehow imposed...evolution to illuminate biology and enable next-generation therapeutics. Prime editing, base editing, PACE...

  4. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    Published
    June 23, 2020, 1:15 p.m.
    ...academia and industry. As a result over 90% of Addgene’s COVID-19 resources are now available to scientists...these groups were willing and able to listen to Addgene’s new approach of working with the industry-based...in the background became a normal occurrence as Addgene and our university partners quickly assessed how...allowed them to synthesize new plasmids quickly and Addgene’s quality control and archiving system allowed us...COVID-19 plasmid collection Beyond COVID-19: Addgene’s plasmid collections available to industry scientists... this alone. If you have deposited plasmids at Addgene and would like to make them available to industry...industry, please reach out at techtransfer@addgene.org and we will initiate a conversation with you and your...

  5. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ...For example, this is an experiment using the chemogenetic tool hM4D(Gi) driven by a GFAP promoter in rat... rat. Contribute your AAV data Just like Addgene’s plasmid repository, the AAV Data Hub is a community-driven...only accepting data for materials distributed by Addgene.  Future directions As the AAV Data Hub grows, ...accelerate your research. Sincerely, Melina & The Addgene Team ...

  6. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...resources on the Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe...Subscribe to the Addgene blog Resources on Addgene.org Meet the other members of Addgene's Board of Directors... did you first hear about Addgene? How did you become involved with Addgene? Katz: Joanne and I first ...connection that got to know more about Addgene and heard that Addgene was looking for new board members. ... that Addgene provides the scientific community? Katz: The most important resource that Addgene provides...What do you hope Addgene will accomplish in the near future? Katz: I’m excited that Addgene is continuing... through Addgene’s sharing platform. Expanding further beyond the US and increasing Addgene’s international...

  8. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...features of Addgene's website Resources on Addgene.org Try out the new search on Addgene's homepage ...Did you see Blugene’s make-over and our new homepage? If you've visited Addgene’s homepage recently, ...been working on behind the scenes here at Addgene.  Addgene's new homepage While users are increasingly...emphasize the values that make Addgene unique: community, sharing, and fun. The Addgene repository is built on....  Blugene gets a make-over You may also have noticed another big update on the homepage. Blugene, our... ways, we realized that Blugene needed the same thoughtful treatment. Blugene has been with us since 2011...might notice Blugene looking sad, especially if an experiment doesn’t go as planned. Blugene also has full...

  9. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

    Type
    Blog Post
    Published
    April 5, 2016, 2:30 p.m.
    ...Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s incredibly important for scientific...distribution and disseminate knowledge around the globe. Addgene’s progress in international distribution As you ...are making great progress toward spreading the Addgene community into developing regions like South America...the countries that have requested plasmids from Addgene since our inception in 2004. In the near future...SeedingLabs or on Facebook Additional Resources on the Addgene Blog Learn About Our Recent Visit to China Read...People at Scientific Conferences Resources on Addgene.org Read about Global Plasmid Distribution Check... Institutions that Deposit their Plasmids with Addgene ...

  10. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...including: generating single or multi-gene knockouts in a wide variety of cell types, gene editing using...efficiency, requires specialized equipment, such as a gene gun. Depending on the nature of the experiment, ... CRISPR-Cas9 Endonuclease for Cell-Type-Specific Gene Editing." Journal of the American Chemical Society...using homology directed repair (HDR), and generating large genomic deletions. What are the advantages of...and specificity is a concern, such as knockout generation or homologous recombination.  However, experiments...step in this type of CRISPR experiment is the generation of the RNP complex.  While one option is to purchase...in vitro transcribed from ssDNA, which can be generated by commercial vendors such as IDT. These two components...

  11. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...overexpress genes of interest; more recently we have also been using Addgene CRISPR vectors to generate cell ...cell lines with knockouts of specific genes. Many years ago, people in the laboratory became frustrated with...cDNA is co-transfected at a favorable ratio to the gene of interest (or, if your protein is fused with a...with different levels of expression of our gene of interest. Advantages of the agarose cloning method ...figure 1.   Find Additional Useful Protocols on Addgene's Protocol Pages Agarose cloning method procedure...-well cell plate to a 96-well assay plate. We generally freeze at least 2 ampules each of 4 clones with...som.umaryland.edu.   Additional Resources on the Addgene Blog Tips for a 1st Time CRISPR User (by a 1st...

  12. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...qPCR is used in a variety of applications including gene expression profiling, studying copy number variation... is also used in high throughput SNP genotyping, gene deletion analysis, and RNA detection.  A summary...starting template DNA concentration Gene expression profiling Copy number variations Quantify RNA levels...Disease or pathogen identification SNP genotyping Gene deletion Analysis RNA detection     References...in colony PCR, or for generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase...101: Colony PCR Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase Chain ...concentration of the original template DNA in the sample.  Addgene currently uses ddPCR for AAV titrating. You can...

  13. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ....1216880109 Resources on Addgene.org CRISPR Plasmids – Protein Tagging CRISPaint Gene Tagging Plasmids 101...Resources on the Addgene blog Plasmids 101: Protein Tags Plasmids for Endogenous Gene Tagging in Human...kDas up to ~35 kDa and are typically knocked-in via gene targeting or cloned in-frame to the protein of interest... protease is situated between the degron and the gene of interest and the degron domain is terminal. Between...sequence (7 kDa). This system also requires less genetic engineering than some of the other systems reviewed... leakiness associated with the system, but the general concept of the system has remained the same. An...

  14. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...contain a toxin gene (regulated by a rhamnose-inducible promoter) and a chromoprotein gene. Allelic exchange...the plate. For example, if your plasmid contains a gene encoding a blue chromoprotein, after transforming...3: pTOX plasmids expressing amilCP and tsPurple genes. Image: Lazarus et al., 2019. One such lab is...protein family. What sets them apart is the way they generate their color: they strongly absorb visible light...difficult to directly use in bacteria, where most genetic manipulations start. Anthony Forster’s lab used...recombination events: The plasmid carrying the desired genetic modification integrates into the genome (Figure...art, let us know! Additional resources on the Addgene blog Find our Fluorescent Protein 101 blog series...

  15. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ... the world, it can be hard to find your favorite gene, promoter, serotype, or a myriad of other things...Retroviral) Species: Specify the source organism for a gene insert. (e.g. S. cerevisiae) Plasmid Type: Choose...to find plasmids matching your search terms with gene and guide RNA / shRNA inserts Resistance Marker:...Resistance: Use this to choose the antibiotic resistance gene(s) used for selection in bacteria. Has Viral Service...terms in an Addgene plasmid search and found the results confusing, I’m sure you’ll find Addgene’s newly launched...Additional resources on the Addgene blog Learn more about other features on Addgene's website ...search, we’ve also revamped our homepage and mascot Blugene (check back on December 5 to learn more about this...

  16. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...expression of a gene. Screen: A library of gRNAs with a CRISPR nuclease target multiple genes in a cell population...’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook. This new edition of the eBook...CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease, or both. Applications...

  17. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...Utilization During Gene Targeting with Zinc-Finger Nucleases. G3 Genes Genomes Genetics, 3(4), 657–664. ...Jaenisch, R. (2013). One-Step generation of mice carrying mutations in multiple genes by CRISPR/CAS-Mediated ...example, choosing target sites that span the knock-in gene. For making mutations, one good way is to mutate...be okay if you are introducing a selection marker gene. The homology arms should be no more than 100 bp...for my genomic PCR, but couldn't amplify the EMX1 gene using same primer you used in the Science paper ...Winderbaum, J., Nickoloff, J. A., & Jasin, M. (1998). Gene Conversion Tracts from Double-Strand Break Repair...Additional resources on Addgene.org CRISPR guide CRISPR protocols by Addgene depositors gRNA design software...

  18. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    Published
    July 28, 2020, 1:15 p.m.
    ...experiment? What if after receiving the plasmid from Addgene you could directly start your digest, PCR, or transformation...preparation is quality controlled through next generation sequencing and spectrophotometry to test purity...Suggestion Form.  Additional resources on the Addgene blog Browse blog posts from our Plasmids 101 blog...the various ways to quantify DNA Resources on Addgene.org Learn about our other beta projects Read our...

  19. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ... to the Hot Plasmids episode! Expanding the optogenetics toolbox with CRY2clust Article contributed by...Basic Science (IBS) has developed another useful optogenetic tool that enables robust and efficient oligomerization...CRY2clust, when integrated into a Ca2+ -modulating optogenetic system (OptoSTIM1), caused two-fold faster changes...soCoChR: A novel opsin that enables single-cell optogenetics Article contributed by Meghan Rego  Listen ...Listen to the soCoChR podcast segment Optogenetic stimulation is a powerful tool used by neuroscientists to...promoters and/or fluorescent tags are available at Addgene. Shemesh OA, et. al. Nature Neuroscience. 2017...The design use to detect PKA activity can be generalized to measure phosphatase activity, second messenger...

  20. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    Published
    Feb. 26, 2019, 1:40 p.m.
    ...opportunity. That's how Addgene was founded. That's how my position and my team at Addgene was created.”- Eric...discussed on the Addgene blog, consider writing a guest post. Additional resources on the Addgene blog Read...transferable skills guide Resources on Addgene.org Find job listings at Addgene Get more career insight from Addgenies...  We touch upon these topics frequently in the Addgene blog. In fact, we have so many blog posts about...into one comprehensive, downloadable guide: the Addgene Science Career Guide. We also asked Addgenies what...

  21. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...the antibiotic resistance gene and origin will replicate, and the transgene will be lost. Over time, bacteria...vector backbone contains the antibiotic resistance gene (AmpR) and origins of replication (ori and f1 ori...these cloning strains, browse the table of common gene mutations in our Plasmids 101: Common Lab E. coli...Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid...recombination, one resulting plasmid carries the transgene of interest (aka the viral genome) and the other...10.1186/s12934-020-01321-4   Kolodner R (1980) Genetic recombination of bacterial plasmid DNA: electron...10.1261/rna.069286.118 Additional resources on the Addgene blog Find more molecular biology and viral vector...

  22. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    Published
    May 25, 2021, 2:29 p.m.
    ...2018), and hybridomas can lose gene expression of the mAb-encoding genes, die, or fail to revive after ...broadly, advances in gene synthesis, affinity reagent screening, and next-generation sequencing of hybridomas...grants and Addgene’s mission This is the first NIH grant that Addgene has received. Addgene prides itself...allow Addgene to further our mission of scientific sharing,” says Dr. Daniela Bourges, Addgene’s interim...opened the door for a new generation of recombinant affinity reagents. Addgene is looking forward to working...antibodies Resources on Addgene.org Find recombinant antibody plasmids at Addgene ...to improve upon these critical research tools. Addgene is excited to announce that we have recently received...

  23. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...targeting a gene associated with diphtheria toxin lethality and were sorted into single wells to generate clonal...instance, it’s much more difficult to introduce a gene of interest into primary cells, so most primary ... risk of viral integration causing mutations and gene expression changes elsewhere in the genome. Second...j.cell.2015.03.028. PubMed. Find the Plasmids @Addgene: iTOP-tested proteins and reporters Previous...

  24. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

    Type
    Blog Post
    Published
    Nov. 12, 2014, 3:54 p.m.
    ...simultaneous activation and/or repression of multiple genes, and more. The researchers now plan to adapt the...powerful tools to modify the expression of multiple genes at the same time.” For more details on how to apply...the Mulitplex CRISPR/Cas9 Assembly System Kit at Addgene here. Other Popular CRISPR Posts: How to Design...

  25. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...protein coding genes in E. coli and S. cerevisiae expression vectors are available at Addgene. A set of mammalian...yeast expression constructs contain protein coding genes, tags, truncations, and codon optimizations similar...of plasmids containing SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae expression vectors ... available at Addgene Read about SARS-CoV-2 detection methods Resources on Addgene.org Find all plasmids...been functionally tested by Ginkgo Bioworks or Addgene. If you used plasmids from this collection in your...COVID-19 Plasmid Collection Quality control at Addgene in the midst of COVID-19 We received Ginkgo’s first... our standard full plasmid sequencing by NGS. Addgene’s QC scientists immediately screen these plasmids...

  26. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    Published
    July 18, 2017, 12:41 p.m.
    ...in applying gene technology to bacteria and plants. Her research involves cloning cry genes from the soil... shared mission of both Seeding Labs and Addgene. The Addgene-Seeding Labs plasmid grant is a collaborative...of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for.... Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check...introduce the 5 scientists who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists...plant biology, she coordinates the Network for the Genetic Improvement of Cowpea, a knowledge database for...potential agricultural and medical use. Find More Optogenetic Tools Like pDawn  Making cassava plants resistant...

  27. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    Published
    Oct. 30, 2018, 1:04 p.m.
    ...expression of four embryonic genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated from mouse fibroblasts...differentiate into specialized cell types [2]. We general speak of 4 levels of stem cell potency: Totipotency... in many organs in the adult human body. These generate specialized cells for their location, mostly to...isolated from the human body Usage Universal. Can generate all adult cell types. Limited. Only specific cell... realistic options for scientists working in regenerative medicine, but the longing for a model system...Yamanaka and Thomson labs showed the successful generation of human iPSCs from dermal fibroblasts via retroviral...Prize for Dr. Yamanaka in 2012. Find plasmids for generating iPSCs iPSCs and ESCs closely resemble each other...

  28. Hot Plasmids Podcast Episode 3: Optogenetics Tools, Improved CRISPR Base Editors, & More!

    Type
    Blog Post
    Published
    Oct. 25, 2017, 1 p.m.
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional... Plasmids podcast series, you'll learn about optogenetics tools for controlling protein activity, methods...Base Editors Hot Plasmid 4: pPSU Plasmids for generating DNA molecular weight markers.  Listen to the ...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ... optogenetics...

  29. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...inhibition library that inhibits over 18,000 human genes and is used for genome-wide inhibition screening...types, recombinases allow you to further restrict gene expression to areas in which the recombinase and... patterns, thus allowing you to further restrict gene activation to cells expressing both Cre and GFP....pAAV-hDlx-Flex-GFP-Fishell_6 Gordon Fishell   Optogenetics Optogenetics continues to supply neuroscience with ...mCherry-Fishell-3 Gordon Fishell   Chemogenetics Chemogenetics offers noninvasive methods for manipulating...help@addgene.org with the vector name and serotype you’d like to be notified about. Chemogenetics Plasmid...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...

  30. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...for the generation of one or more mutations – it is as easy as obtaining a plasmid with the gene of interest...interest (search Addgene plasmids by gene here), designing primers with your desired mutation, performing... desired promoter, selectable marker, and target gene, for example, are amplified with overlapping regions... E.coli for plating, screening and generation of sgRNA. Generation of sgRNA libraries, with maximum complexity...multiple mutations in one or multiple fragments, and generate constructs for producing single-guide RNAs – but...the NEBuilder HiFi DNA Assembly Mix include:  Generation of constructs with multiple point mutations Cloning...sgRNAs Production of Yeast Expression Cassettes Generating constructs with multiple point mutations NEBuilder...

  31. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... nucleic acid sequence of the antibody-encoding genes over time as the hybridoma cells divide. These changes...before culturing or they can clone the genes from the hybridoma into a plasmid to create a recombinant...Figure 2: Changes to the heavy chain or light chain genes within a hybridoma impacts the specificity, avidity...Resource on Addgene.org Check out Addgene’s antibody plasmid collection Read the latest on Addgene's new antibody...above, genetic drift is one concern when using hybridomas to produce monoclonal antibodies. Genetic drift...the target molecule. Although it’s possible to generate antibodies against many types of molecules including...recognize proteins. Figure 1: Steps in the generation of hybridomas for monoclonal antibody production...

  32. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...her current research, Sheen aims to identify new genes related to PZA-resistance by using different approaches...as pACBSR-hyg and pFLP-hyg will be used to create gene deletions of important unknown regulatory regions...growth will then be measured to link the deleted genes to specific functions in biofilm formation. The...on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out Addgene-Seeding...of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for...Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources, equipment...resource-limited settings. More specifically, the Addgene-Seeding Labs plasmid grant provide plasmids to ...

  33. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ...exhibiting its versatile utility in quantifying multiple gene editing activities. Click here to find the ACE ...Wanisch  Listen to the optogenetics podcast segment LOVTRAP is an optogenetic system that was recently... the University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 and Zdk1, where...function is more specific and much faster than any genetic or pharmacological manipulations on cells, and ... riboswitches used in the Riboglow platform at Addgene. Braselmann E, et al. Nature Chemical Biology...

  34. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type
    Blog Post
    Published
    July 2, 2015, 8:37 p.m.
    ... we can make Addgene better and make scientists’ lives easier. Why transition to Addgene? So why did ...more satisfying, because Addgene is a non-profit, we can focus more on generating blog content that scientists... that PhD. Resources at Addgene Read Other Careers Posts Visit the Addgene Careers Page Learn about ...Harvard University and entered a new role here at Addgene as an Outreach Scientist. I used to spend my days... less time wrangling bacteria and instead help Addgene fulfill its mission of helping scientists share...did I join Addgene? Was I jaded by my time in academia? Did I run screaming from the lab like the cartoon...didn’t, it would have been unwise of me to join Addgene. This is particularly true in my position as an...

  35. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...domain due to a gene that resembles cas10. All type IV subtypes are missing adaptation genes, Cas1 and Cas2...sequence similarity, phylogenetic analysis, neighborhood analysis of surrounding genes, and experimental ...straightforward as it seems. There is not a single gene that is shared across all CRISPR types; the fast...together because they contain a distinct Cas7-type gene. The exact mechanism of type IV systems is not known... and applications with the type I CRISPR system. Gene and Genome Editing, 3–4, 100013. https://doi.org...the array of CRISPR technologies. Learning the general principles of this classification system will help...A–C, with the beloved SpCas9 (from Streptococcus pyogenes) belonging in II-A. Cas9 was the first to be adapted...

  36. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...’s first genetic engineering company, Genentech, was founded.  This commercialization of gene editing ...before the discovery of genes themselves.  How has scientific progress in genetic editing impacted the world... DNA sequences is not ideal. For the early generation gene editors, new amino acid recognition sites had...followed by non-homologous end-joining that results in gene knockouts.   Star Trek: Into Darkness (2013) which...The major drawback of all the endonuclease-based gene editors (meganucleases, ZFNs, TALENs, and CRISPR-Cas...Gregor Mendel on the genetic inheritance (1866) and just when the Mendelian genetics was being revived by...Zinc-Finger Nucleases. Genetics 188:773–782 . https://doi.org/10.1534/genetics.111.131433 Doudna JA, Charpentier...

  37. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ...URL “http://n2t.net/addgene:87155” where the Addgene registered prefix and the Addgene ID # are directly...Daniel Bauer (Addgene plasmid # 87155 ; http://n2t.net/addgene:87155 ; RRID:Addgene_87155).” So when citing...Daniel Bauer (Addgene plasmid # 87155 ; http://n2t.net/addgene:87155 ; RRID:Addgene_87155)” Cite plasmid...through Addgene and find verified information about each plasmid item. Given a specific Addgene ID # listed...available through Addgene. Let’s take a look at lenti-Cas9-VQR-Blast, which has the Addgene ID #87155. The... as “RRID:Addgene_87155” and the following URL (https://scicrunch.org/resolver/RRID:Addgene_87155) will...contain the Addgene ID # and the information necessary to indicate that this ID is managed by Addgene. How to...

  38. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...when trying to edit a gene using homology directed repair, since HDR-mediated gene editing is most efficient...formerly Cpf1), a nuclease that generates double strand breaks in target genes resulting in the formation ...always positioned correctly to target a particular gene. Furthermore, a target sequence may have high homology...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog  Read more about Cpf1...sequences While PAM sequences for the commonly used S. pyogenes Cas9 (3'-NGG) are abundant throughout the human...circumvent this limitation: 1) the use of novel S. pyogenes Cas9 variants with varying PAM sequences, 2) the...Cas9 homologs derived from species other than S. pyogenes, and 3) the use of non-Cas9 enzymes. (For more...

  39. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...edits of interest than solely transfected cells. “Gene On” (GO)- a functional reporter system to identify... (Katti et al., 2020). They named this reporter “Gene On” or GO. GO works by affecting protein translation...fluorescent proteins. Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter...and enrich for base-editing events in vivo. TLDR: Gene On (GO) from the Dow lab is a flexible reporter ...base-pair mCherry insertion. Editing of these motifs generates lesions that are subject to excision and ssDNA...This edit changes the histidine to a tyrosine generating a GFP variant (GFPY66) that has a shifted emission...hypothesis with GFP fluorescence. To do this they generated a silent GFP construct, that contains an ACG start...

  40. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ... maintain the genes they carry among organisms belonging to many different phylogenetic groups (Jain and...that the plasmid is conjugative and carries the genes necessary for transfer initiation between bacterial...plasmids for DNA cloning in Gram-negative bacteria. Gene, 70(1), 191–197. https://doi.org/10.1016/0378-1119... S. (2011). Broad-host-range plasmid vectors for gene expression in bacteria. Methods in Molecular Biology...viscosus, Aerobacter aerogenes, Aeromonas hydrophila, Agrobacterium tumefaciens, Alcaligenes eutrophus, Azotobacter...salmonicida, Agrobacterium tumefaciens, A. rhizogenes, Alcaligenes eutrophus, Enterobacter sp., Erwinia amylovora... broad host-range plasmid RK2. Molecular & General Genetics: MGG, 152(3), 129–135. https://doi.org/10.1007...
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