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We narrowed to 608 results for: Sis

Showing: 361 - 400 of 608 results

  1. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...Fluorescent Protein? GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish...began engineering new versions of GFP through mutagenesis in order to improve its physical and biochemical...

  3. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...however, the protein integration host factor (IHF) assists with the process: it bends the DNA to redistribute...with a beneficial function (usually antibiotic resistance).  In the lab, low copy plasmids may result in...

  4. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...Susanna Stroik The Liu lab recently used phage-assisted continuous evolution and protein engineering to...  Doman, J.L., Pandey, S., et al. (2023). Phage-assisted evolution and protein engineering yield compact...

  5. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...screening, followed by sequencing and further data analysis. Based on Figure 31.5.2 from https://pubmed.ncbi.nlm.nih.gov...scripts provided by the depositing lab for data analysis.  I'm often asked whether this step can be skipped...

  6. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...activators The simplest of the dCas9 activators consists of dCas9 fused to a single transcriptional activation...Mediator complex, generally the strongest activator, consists of a dCas9-VP64 fusion and a modified gRNA that...

  7. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ... well received, understand that people can be resistant to change. Ask your peers or your superiors to...to explain their rationale when they’re resistant to new ideas. If their answer is “we’ve always done ...

  8. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...environmental pollutants, drug discovery, and disease diagnosis. As biosensors, aptamers hold a few important...largely in vitro; Easy and reproducible commercial synthesis (those of us who have used antibodies for diagnostics...

  9. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ... (BDNF). BDNF is a small protein involved in neurogenesis, neuronal survival, and synaptic plasticity....Groves, J. O. "Is it time to reassess the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (...

  10. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...not need to be grant aims. Each aim could be the basis of a single grant. Either way, think realistically...tools? A novel dataset? Unique expertise? A novel hypothesis? Part of the motivation for doing the research...

  11. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...the hippocampus. This technology has formed the basis of the Rett syndrome program being pursued at Vico...nature24058 Tillotson R, Bird A (2020) The Molecular Basis of MeCP2 Function in the Brain. Journal of Molecular...

  12. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...alternative tRNAs Codon optimization With low cost DNA synthesis, one of the primary ways researchers solve the...created codon optimization algorithms and DNA synthesis companies such as IDT and GenScript host online...

  13. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...image shows that the vast majority of the vectors consist of full particles (white arrowhead) relative to...Droplet Digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense...

  14. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...be made easily and cheaply using oligo library synthesis. Prior to Cas9 activators, similar tools were ...function (Pan et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences ...

  15. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...content.      References 1. Cho, Seung Woo, et al. "Analysis of off-target effects of CRISPR/Cas-derived RNA-guided... et al. "Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes....

  16. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...provenance (this is the history of the cell line consisting of records of the origin and propagation of the...Transition Is Required for Acquisition of Anoikis Resistance and Metastatic Potential in Adenoid Cystic Carcinoma...

  17. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...occasionally beaten to press, as there is so much analysis possible. Most scientists are, however, privileged...requirements and likely will not do so (calling it a “sisyphean task” given the endless types of data). Rather...

  18. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ..., Y., & Peng, C. (2018). Overview of MicroRNA biogenesis, mechanisms of actions, and circulation. Frontiers...Ørom, U. a. V. (2023). Recent progress in miRNA biogenesis and decay. RNA Biology, 21(1), 36–43. https:/...

  19. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ... transmitted through the integrin, where it is resisted by the ligand embedded in the extracellular environment...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...

  20. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...physical structure, separate chromosomes during mitosis, support transport of organelles and vesicles, ...the samurai sword called a katana. Opto-katanin consists of two separate blue light-sensitive systems, ...

  21. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ... et al. 2022). Lactate is a key metabolite in glycolysis, so this sensor will be a valuable tool to study...lifetime imaging. B) LiLac responses are highly resistant to pH changes. Image adapted from Koveal et al...

  22. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...modification of interest, ordering them from a gene synthesis company such as IDT or Genscript, and cloning ...using adeno-associated virus (AAV)." Chromosomal Mutagenesis. Humana Press, New York, NY, 2015. 75-103. PubMed...

  23. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ... set of mutations that made cancer cells more resistant to chemotherapy. You can find these plasmids at.... Thus yeast can be used to study the molecular basis of human diseases that are caused by a specific ...

  24. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ... steps: 1. Define your timeline Start with your thesis in mind. Imagine the kind of data your dream discovery...consultant and coach in Boston, MA. He specializes in assisting research scientists create productive and manageable...

  25. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...optogenetic tools derived from direct comparative analysis of microbial opsins. Nature Methods, 9(2), 159...

  26. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...Aschauer, D. F., Kreuz, S., & Rumpel, S. (2013). Analysis of Transduction Efficiency, Tropism and Axonal...Hosokawa, Y., & Konishi, H. (2014). A comparative analysis of constitutive promoters located in adeno-associated...

  27. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...generate more signal from low abundance targets. Analysis/Presentation Once you’ve captured your images,...imaging approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the...

  28. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...advantage of CRISPR epigenetic tools is their persistence and inheritance. CRISPR activators and repressors... culture. However, DNMT3A-induced methylation persisted throughout a 100 day experimental period, as this...

  29. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...be used for numerous applications, including transgenesis, gene-trap screens, and gene editing. How does... Verma, “Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient...

  31. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...protein tags. The basics These tags most often consist of an enzyme that has been mutated to block its...Labeling Technology for Cell Imaging and Protein Analysis. ACS Chemical Biology, 3(6), 373–382. https://...

  34. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...distinguish different target proteins in your flow analysis, you'll need to use fluorophores that attach to...you can separately detect these markers in your analysis. Lastly, there are fixable dye stains that react...

  36. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...the complexity of the screen. For example, the analysis becomes easier, the number of cells needed is ...add value if you are addressing a well-defined, hypothesis driven research question. Examples of custom ...

  37. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...contributed by guest blogger James D. Fessenden, an Assistant Professor at Brigham and Women’s Hospital. Biochemists...Fessenden! James D. Fessenden is currently an Assistant Professor in the Anesthesia Research department...

  39. We’re Going Viral This Week!

    Type
    Blog Post
    Published
    Oct. 14, 2019, 1 p.m.
    ...strategies to improve viral vector production and data analysis. Our first paper came out last year on sucrose...

  40. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...itself. Many of us have experienced the minor yet consistent annoyance of walking into a fly lab and finding...
Showing: 361 - 400 of 608 results