We narrowed to 602 results for: ACE

1. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

   Type

   Blog Post

   Published

   June 17, 2021, 1:17 p.m.

   ... plasmid with your gene of interest, you would replace mCherry with your gene of interest. pBLADEONLY:...

2. The Golden Stopwatch Award: Recognizing Facilitators of Research

   Type

   Blog Post

   Published

   Sept. 5, 2019, 1:06 p.m.

   ... a material, a deposit agreement must be put in place. And subsequently, every time that material is requested...

3. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

   Type

   Blog Post

   Published

   Aug. 8, 2019, 1:19 p.m.

   ...their journal. The early peer-review attempts took place at the beginning of the 18th century. Yet the peer-review...

4. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

   Type

   Blog Post

   Published

   April 25, 2019, 3:01 p.m.

   ...a protein which had a total of 31 amino acid replacements relative to CFP484. Because the excitation and...

5. Plasmids 101: Origin of Replication

   Type

   Blog Post

   Published

   Nov. 11, 2020, 2:23 p.m.

   ...and all of its control elements. The ori is the place where DNA replication begins, enabling a plasmid...

6. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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   Blog Post

   Published

   Sept. 17, 2020, 1:15 p.m.

   ...subsequent fluctuations in distribution. I don’t have space in this blog to call out every Addgene Team because...

7. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

   Type

   Blog Post

   Published

   Dec. 13, 2018, 1:14 p.m.

   ...affinity for heparan sulfate proteoglycans on the surface of the packaging cells (Vandenberghe, 2010). Rather...

8. Using Addgene's New Viral Service for Your Research

   Type

   Blog Post

   Published

   Nov. 17, 2016, 3:30 p.m.

   ...libraries. Whether you’d like to knockout a gene, replace it, modulate its expression, or perform a genome-wide...

9. Intro to Our Summer SciComm Series!

   Type

   Blog Post

   Published

   June 9, 2022, 1:15 p.m.

   ...and intent to the messaging - while also creating space for people outside of the primary audience to engage...

10. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type

    Blog Post

    Published

    June 9, 2016, 2:30 p.m.

    ...the question on everyone’s minds is: could NgAgo replace CRISPR? Such a drastic shift won’t happen overnight...

11. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type

    Blog Post

    Published

    Oct. 29, 2015, 2:30 p.m.

    ...EPISOME-MEDIATED TRANSFER OF DRUG RESISTANCE IN ENTEROBACTERIACEAE VIII: Six-Drug-Resistance R Factor.” Journal...

12. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type

    Blog Post

    Published

    July 7, 2015, 3:36 p.m.

    ... CRISPR/Cas9 applications, SEC selection could replace other, more restrictive C. elegans selectable marker...

13. Another Pathway into Cells: iTOP

    Type

    Blog Post

    Published

    June 23, 2015, 8:37 p.m.

    ...feasible for many labs.  References Efficient Intracellular Delivery of Native Proteins. D'Astolfo DS, Pagliero...

14. Mentoring for Scientists: I Have a Mentor, What Now?

    Type

    Blog Post

    Published

    March 6, 2014, 7:12 p.m.

    ...Choosing a Good Mentor", and more! Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...

15. The MTA at Addgene

    Type

    Blog Post

    Published

    July 23, 2024, 2:17 p.m.

    ...request going forward.  After another scientist has placed a request for your plasmid, they must complete ...

16. Creating accessible biology activities in schools with BioBits

    Type

    Blog Post

    Published

    Aug. 2, 2018, 12:56 p.m.

    ...different machines are, and how many regulations are in place to keep everyone safe. None of this is conducive...

17. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type

    Blog Post

    Published

    Sept. 19, 2017, 1:20 p.m.

    ...phototoxocity is induced when expressed on the cell surface, in the cytosol, in mitochondria, or in cell nuclei...

18. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type

    Blog Post

    Published

    July 21, 2017, 12:48 p.m.

    ... systems in Drosophila: A synthesis of time and space, Trends Genet. 20, 384–391 (2004). PubMed PMID: ...

19. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type

    Blog Post

    Published

    June 4, 2019, 1:02 p.m.

    ...how bacteria interact with each other and plant surfaces. His system of choice is the Arabidopsis thaliana...

20. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type

    Blog Post

    Published

    May 21, 2019, 12:10 p.m.

    ... to die or cells dying at the wrong time and/or place can lead to an array of diseases and pathologies...

21. Improving Transduction Efficiency with Exosome AAVs

    Type

    Blog Post

    Published

    March 14, 2019, 1:54 p.m.

    ...particles are either associated with the outside surface of the exosome or are carried inside the vesicle...

22. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type

    Blog Post

    Published

    April 9, 2020, 1:15 p.m.

    ...Twitter (QBI_UCSF and KroganLab), Instagram, and Facebook. Find more COVID-19 and coronavirus related plasmids...

23. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type

    Blog Post

    Published

    Dec. 20, 2018, 1:15 p.m.

    ...established ethical issues raised around mitochondrial replacement therapies. In other words, rather than having...

24. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type

    Blog Post

    Published

    Oct. 26, 2017, 1:59 p.m.

    ...commonly uses a custom script derived from the count_spacers.py file from Feng Zhang’s laboratory. There are...

25. Plasmids 101: Gram Negative and Positive Bacteria

    Type

    Blog Post

    Published

    Aug. 2, 2022, 1:15 p.m.

    ...Rinse briefly with tap water. Add ethyl alcohol or acetone drop by drop onto the smear until the alcohol runs...

26. DAP arrays for multiplex gene editing

    Type

    Blog Post

    Published

    July 7, 2022, 1:15 p.m.

    ...array encoding the tandemly assembled tRNA-gRNAs is placed downstream of a lengthy promoter, which is used...

27. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type

    Blog Post

    Published

    June 23, 2022, 1:15 p.m.

    ...Application in Genetic Diseases: A Review.” Current pharmaceutical biotechnology. https://doi.org/10.2174/1389201021666200621161610...

28. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type

    Blog Post

    Published

    April 7, 2022, 1:15 p.m.

    ...sensor to the membrane and displays it on the extracellular side. In addition to the original PDGFR TMD,...

29. Four Factors that Differentiate the Stem Cell Field

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    Blog Post

    Published

    Sept. 28, 2016, 2:35 p.m.

    ...communities, but usually I only get to scratch the surface of different fields. This meeting has given me ...

30. Addgene Welcomes John Doench to the Advisory Board

    Type

    Blog Post

    Published

    Aug. 13, 2015, 2:30 p.m.

    ... need to spend time here, this is an incredible place.” Doench has spent most of his research career working...

31. Fluorescent Proteins 101: Luciferase

    Type

    Blog Post

    Published

    June 24, 2014, 3:59 p.m.

    ...Phrixothrix hirtus), and a variety of marine crustaceans. Many of these luciferases have been subjected...

32. Advice for Scientists Starting a Lab

    Type

    Blog Post

    Published

    April 29, 2014, 1:43 p.m.

    ... who was always “off flying his plane to exotic places while occasionally emailing us his worldly advice...

33. Viral Vectors 101: Types of viruses

    Type

    Blog Post

    Published

    June 6, 2023, 1:15 p.m.

    ...are roomy: some adenoviruses pack up to ~35 kb of space for cargo! Adenovirus is an effective option if ...

34. Viral Vectors 101: Viral Applications

    Type

    Blog Post

    Published

    April 13, 2023, 1:15 p.m.

    ...human transcription factors, barcoded libraries to trace cell lineage, and so much more! Libraries allow ...

35. Sharing is Caring: But How to Distribute Open Hardware?

    Type

    Blog Post

    Published

    Feb. 28, 2023, 2:15 p.m.

    ...Distributing hardware via globally established makerspace networks and training users via workshops, seminars...

36. How Addgene Creates Content

    Type

    Blog Post

    Published

    Nov. 17, 2022, 2:15 p.m.

    ...over their favorite science topics. What better space to do that than in the Addgene blog?  Fig...

37. Plasmids 101: Screens vs. Selections

    Type

    Blog Post

    Published

    Nov. 10, 2022, 2:15 p.m.

    ...raising or lowering the culture temperature to exacerbate a mutant phenotype, very similar to enhancer/...

38. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type

    Blog Post

    Published

    July 28, 2017, 1:30 p.m.

    ...process is fairly straightforward. Basically, you place your gene of interest in a plasmid and get the vector...

39. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type

    Blog Post

    Published

    July 6, 2017, 1:59 p.m.

    ...the upregulation of the immunosuppressive cell surface receptor PD-1. This immunosuppressive environment...

40. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type

    Blog Post

    Published

    May 11, 2017, 2:30 p.m.

    ...Learn About our Cancer Colleciton Pages Use the ClonTracer Library for Lineage Tracing Learn About Cancer...

41. Multiplex Genome Editing with CRISPR-Cpf1

    Type

    Blog Post

    Published

    May 9, 2017, 2:12 p.m.

    ...Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer Multiplexing CRISPR-Cas9 options before Cpf1...

42. Addgene’s Viral Service - Why Virus? Why Now?

    Type

    Blog Post

    Published

    March 23, 2017, 2:30 p.m.

    ...will help scientists Good science isn’t the only facet of a project this big. Maintaining our excellent...

43. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type

    Blog Post

    Published

    June 25, 2019, 12:54 p.m.

    ...nature, this genetic material often comes from adjacent lysed bacteria and can include plasmid DNA or ...

44. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    Published

    June 6, 2019, 12:41 p.m.

    ...sometimes require an alternative such as a radioactive tracer. As a solution, Mikhail Shapiro’s lab has developed...

45. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type

    Blog Post

    Published

    May 19, 2020, 1:15 p.m.

    ... the current global pandemic in which scientists race to find vaccines, therapeutics, and other treatments...

46. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type

    Blog Post

    Published

    Jan. 30, 2020, 2:15 p.m.

    ... and not a false negative. Therefore, they will place special emphasis on positive controls and good sample...

47. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ...Engineered CRISPR-Cas9 nuclease with expanded targeting space." Science 361.6408 (2018): 1259-1262. PubMed PMID...

48. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type

    Blog Post

    Published

    Feb. 19, 2019, 1:36 p.m.

    ...making sure we have adequate process and controls in place to manage those scenarios. What is the best piece...

49. Early Career Researcher Toolbox: Social Media for Scientists

    Type

    Blog Post

    Published

    Dec. 11, 2018, 12:46 p.m.

    ...post provides suggestions for using LinkedIn and Facebook. For each of the three social media platforms,...

50. Plasmids 101: Inducible Promoters

    Type

    Blog Post

    Published

    Jan. 18, 2018, 2:34 p.m.

    ...and allowing reporter gene transcription to take place. Addgene also has yellow flame light-regulated two...