We narrowed to 609 results for: CaS;

1. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

   Type

   Blog Post

   Published

   Dec. 5, 2019, 1:49 p.m.

   ...expression. You’ll mostly see a friendly smile, but occasionally you might notice Blugene looking sad, especially...

2. Working 9-5: an Addgene Co-op

   Type

   Blog Post

   Published

   June 2, 2022, 2:15 p.m.

   ... would feel isolating, but that has not been the case for me at Addgene. There is a clear effort to make...

3. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

   Type

   Blog Post

   Published

   March 10, 2016, 3:30 p.m.

   ...the Day feature to our home page - each day we showcase a new plasmid that you might otherwise miss. We...

4. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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   Blog Post

   Published

   July 29, 2014, 6:16 p.m.

   ...a great collection with such variety.” In most cases, backbones are available to attach the gene of interest...

5. An Addgene Summer Internship

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   Blog Post

   Published

   Aug. 8, 2024, 7:22 p.m.

   ...options from the Addgene catalog. These were a miR30 cassette, Syntaxin3, and osa-miR528. My manager, Katherine...

6. Embracing Serendipity: A Crucial Element in the PhD Journey

   Type

   Blog Post

   Published

   April 25, 2024, 1:15 p.m.

   ...often emerges at the intersections of disciplines. Cast your net wider, explore tangential subjects, and...

7. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

   Type

   Blog Post

   Published

   June 29, 2023, 7 p.m.

   ...practices, create an email strategy, and make the occasional TikTok. I was also responsible for typical entry...

8. Cellosaurus: A Cell Line Information Database

   Type

   Blog Post

   Published

   Feb. 21, 2023, 2:15 p.m.

   ...-intensive project; scripts can be used in some cases, but much of this work has required manually going...

9. SunTag and Fluorescent Imaging

   Type

   Blog Post

   Published

   March 28, 2017, 2:30 p.m.

   ...experiments in their paper, and so can you! In almost any case you would use a traditional FP fusion, a SunTag ...

10. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type

    Blog Post

    Published

    June 11, 2020, 1:34 p.m.

    ... experiment. Viral delivery is the same in both cases, but activation depends on small molecule activation...

11. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type

    Blog Post

    Published

    June 4, 2020, 1:22 p.m.

    ... your plasmid at -20°C or even at -80°C, in this case your preparation can be eluted in water or in your...

12. Fostering communication in remote collaborations

    Type

    Blog Post

    Published

    April 21, 2020, 1:14 p.m.

    ...addressed and new ideas generated. Furthermore, this casual communication reflects the type of informal communication...

13. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type

    Blog Post

    Published

    June 11, 2019, 1:16 p.m.

    ...images “Labeled for reuse with modification.” Occasionally Google Image Search results includes images ...

14. Understanding GPCR Signaling Using TRUPATH

    Type

    Blog Post

    Published

    Dec. 14, 2020, 2:15 p.m.

    ...in the initiation of a vast number of signaling cascades, inducing different cellular responses. One of...

15. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type

    Blog Post

    Published

    Dec. 3, 2020, 2:15 p.m.

    ... domains, all four Rep proteins share a common helicase and ATPase domains. To function, the Rep proteins...

16. Management for Scientists: Giving Feedback

    Type

    Blog Post

    Published

    Feb. 6, 2015, 2:46 p.m.

    ...appropriately to get the message across. Listen to our podcast interview with Harvard Medical School researcher...

17. 10 Ways to Share Your Science!

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    Blog Post

    Published

    May 13, 2014, 8:34 p.m.

    ... story and science involves characters – in this case you – on a quest to ask and answer questions and...

18. Advice for Choosing a Research Project

    Type

    Blog Post

    Published

    April 17, 2014, 2:45 p.m.

    ...research project. What will the impact be in a best case scenario? If you get your dream results, what will...

19. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type

    Blog Post

    Published

    Aug. 18, 2023, 4:15 p.m.

    ...mRNA, directly translated into proteins, or, in the case of retroviruses, reverse transcribe their gRNA into...

20. What the HEK?

    Type

    Blog Post

    Published

    April 18, 2023, 1:15 p.m.

    ...produce recombinant proteins at high levels. In a case where clinical applications of recombinant proteins...

21. Antibodies 101: Epitope Availability

    Type

    Blog Post

    Published

    Jan. 11, 2022, 2 p.m.

    ...specific configurations or conditions. In those cases, pay special attention to designing your controls...

22. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type

    Blog Post

    Published

    Sept. 14, 2021, 1:15 p.m.

    ...single colonies and testing multiple preps. In some cases, we suggest gel extraction to isolate the full, ...

23. Science Careers: Unruly Interests Feed Many Paths

    Type

    Blog Post

    Published

    Sept. 26, 2017, 12:12 p.m.

    ...’s scientific discoveries into an entertaining podcast? Look at our multimedia producer and online editor...

24. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

    Type

    Blog Post

    Published

    April 12, 2018, 1:02 p.m.

    ...classroom Engineering the Plant Genome Using CRISPR/Cas9 pSiM24: Simplifying Plant Genetic Engineering Resources...

25. Meet the New Addgene Board Members!

    Type

    Blog Post

    Published

    April 28, 2020, 1:14 p.m.

    ...Therapeutics and is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically test the function of ...

26. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type

    Blog Post

    Published

    Sept. 5, 2019, 1:06 p.m.

    ...deposit agreements to basically instantaneous in many cases.  Suggest that they get an Addgene account for Tech...

27. Identifying Sequence Elements with SnapGene's Feature Database

    Type

    Blog Post

    Published

    Dec. 21, 2017, 2:06 p.m.

    ... crafted a detection algorithm that tolerates occasional mismatches or indels. Empirical tests indicated...

28. The Strength of Story Telling

    Type

    Blog Post

    Published

    Dec. 19, 2017, 2 p.m.

    ...your structure. Develop your protagonist In most cases, the protagonist will be you. Make sure to highlight...

29. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type

    Blog Post

    Published

    Oct. 20, 2020, 1:15 p.m.

    ...series or biotech club, managed their own blog or podcast, initiated and maintained a collaborative project...

30. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type

    Blog Post

    Published

    Sept. 17, 2020, 1:15 p.m.

    ... our operations. We don’t raise money except occasionally for capital equipment or resources needed to...

31. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type

    Blog Post

    Published

    Jan. 24, 2019, 2:24 p.m.

    ...transgene. When multiple copies of the transgene cassette are introduced, stochasticity will result in a...

32. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type

    Blog Post

    Published

    Dec. 13, 2018, 1:14 p.m.

    ...others, such as AAV2, low yields are the norm. In the case of AAV2 this is thought to be due to the viral particle...

33. Plasmids 101: TOPO Cloning

    Type

    Blog Post

    Published

    Oct. 27, 2016, 2:30 p.m.

    ...topoisomerase site inserted into a beta-galactosidase cassette allowing a researcher to perform blue-white screening...

34. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type

    Blog Post

    Published

    Oct. 29, 2015, 2:30 p.m.

    ...to integrate into the E. coli chromosome in some cases. This terminology held until the 1960s when scientists...

35. Advice for Moving Into Sales After Your Science Postdoc

    Type

    Blog Post

    Published

    Aug. 21, 2014, 3:15 p.m.

    ...apply themselves immediately to the position. In case you are interested yourself, sales and marketing...

36. Mentoring for Scientists: I Have a Mentor, What Now?

    Type

    Blog Post

    Published

    March 6, 2014, 7:12 p.m.

    ...after that will lead to learning. Listen to our podcast interview with Harvard Medical School researcher...

37. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    Published

    Oct. 19, 2023, 1:15 p.m.

    ...Recombinant AAV vector with ITRs that flank the gene cassette, which contains a promoter, the gene of interest...

38. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type

    Blog Post

    Published

    June 1, 2021, 1:15 p.m.

    ...collaboration between two research groups. In other cases, the collaborations were simpler and the volunteers...

39. Visualizing Translation at the Single Molecule Level

    Type

    Blog Post

    Published

    Aug. 1, 2017, 1:15 p.m.

    ...dynamics, but they come with a few caveats. In both cases, creating a reporter mRNA requires inserting multiple...

40. The Power Behind NGS Plasmid Validation: seqWell

    Type

    Blog Post

    Published

    April 19, 2017, 3:25 p.m.

    ...this “pre-prep” normalization, it is also often the case that individual libraries prepared from multiple...

41. FPbase: A new community-editable fluorescent protein database

    Type

    Blog Post

    Published

    May 16, 2018, 1 p.m.

    ...after submission. Using FPbase The most obvious use case for FPbase is finding information on a particular...

42. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type

    Blog Post

    Published

    July 21, 2020, 1:15 p.m.

    ...on the next step of your experiment. But in many cases, you won’t see any signs of DNA in your final tube...

43. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type

    Blog Post

    Published

    June 30, 2020, 1:15 p.m.

    ...workshop. “We made sure that we had a backup plan in case someone’s internet cut out. “ On networking Networking...

44. 25 Work From Home Tips for Scientists

    Type

    Blog Post

    Published

    March 19, 2020, 1:15 p.m.

    ...something like puzzling or crafts, while listening to podcasts made for kids. Plan out meals and snacks ahead...

45. Summer SciComm Series: A PhD in Science Communication

    Type

    Blog Post

    Published

    Aug. 23, 2022, 1:15 p.m.

    .... doi:10.1038/nclimate1547 Kunda, Z. (1990). The case for motivated reasoning. Psychological bulletin,...

46. Four Factors that Differentiate the Stem Cell Field

    Type

    Blog Post

    Published

    Sept. 28, 2016, 2:35 p.m.

    ...scientific method cannot address. Listen to Our Podcast Interview with Stem Cell Researcher Darrell Kotton...

47. Management for Scientists: Delegating is Key

    Type

    Blog Post

    Published

    Feb. 20, 2015, 8:30 p.m.

    ...process is completed more efficiently. Listen to our podcast interview with Harvard Medical School researcher...

48. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type

    Blog Post

    Published

    Oct. 9, 2014, 3 p.m.

    ...ones), they can be a good starting point. In many cases it is sufficient to use a spectrum for a closely...

49. Advice for Scientists Starting a Lab

    Type

    Blog Post

    Published

    April 29, 2014, 1:43 p.m.

    ...“off flying his plane to exotic places while occasionally emailing us his worldly advice”, the other was...

50. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type

    Blog Post

    Published

    Sept. 26, 2024, 1:15 p.m.

    ...that share homology in the same reaction. In these cases, the use of another cloning method, such as Golden...