...through smart engineering Different FLP versions – making FLP efficient The first version of FLP discovered...proteins needed for later experiments (cellular tracking for instance) can be controlled with FLP-FRT. ... Cardin, Jessica A. et al. 2009. “Driving Fast-Spiking Cells Induces Gamma Rhythm and Controls Sensory...

...when 2 mismatches are present. The protospacer flanking sequence (PFS) for LshCas13a, which is analogous...have utilized dCas13 fusion proteins for imaging, tracking, modulating splicing, and regulating expression... in length and does not require a specific PFS, making it a very flexible cleavage system. LwaCas13a is...

...speech, coordination, purposeful use of hands, and walking. They may exhibit slow head growth, develop seizures...medical journal. Decades later and after years of painstaking work trying to isolate the causative gene for...:2<147::aid-ajmg6>3.0.co;2-o Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY (2013) An AT-Hook...

...a bit of tuning before you know your system is working as expected. Finally, for each gene you want to... scorer 1.0 (Now 2.0) (Chari et al., 2015) and picking whichever guides are closest to the transcription...Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti...

...that particular region. No matter where you are looking, you can either scan the genome yourself for potential...leading to degradation that can set you back days. Working with plasmid DNA is much easier and doesn’t require...slightly different method to genotype their pups. Taking a toe, a finger, a tail tip, or a combination of...

...we are interested in circuits that mediate drug taking behavior. If a circuit reinforces behavior (activation...sense of pleasure that the animal perceives through taking a drug. The ideal behaviors to test reinforcement...amygdala to nucleus accumbens facilitates reward seeking." Nature 475.7356 (2011): 377-380. Pubmed PMID:...

... this, similar systems have been developed for tracking various cell types and organisms and for improving...recombinase. The XFPs in Flybow are membrane-tethered, making this system useful for visualization of cellular...construct was then used to develop a system for tracking the fate of Langerhans cells. In the Ubow mouse...

...dependency on magnesium and higher thermal stability, making it a potentially more useful tool for in vivo studies...much potential for growth as RNA biology, we are looking forward to reports on the next additions to aptamer...bright, high-affinity complex for RNA labeling and tracking." ACS chemical biology 9.10 (2014): 2412-2420....

...cells of interest. In many experiments you’ll be working with a heterogeneous cell population, for example...While flow cytometry can be used for virtually all kinds of experiments and research fields, for any experiment...investigating negative regulators of T cells and cytokine signaling in tumor immunity. When he's not in ...

...false positives observed in the cloning process, making it easier to produce the constructs you need. Expression...to simplify the protein purification process by making it easy for a researcher to quickly generate N-...carefully chosen to perform statistically relevant benchmarking of cell-free expression systems, and tested ...

...nucleotides, called dideoxynucleotides (ddNTPs), lacking the 3’ hydroxyl group required for the extension...sequencing reactions and may use Sanger sequencing. Looking to the future Southern blotting, restriction mapping...mouse lines, such as BAC transgenic or traditional knockins/knockouts, a thing of the past. In the sequencing...

... however NLuc’s emission peak is at 460 nm thus making it non-optimal for in vivo imaging. The solution...about to create an in vivo imaging reporter by linking two CyOFP1 molecules to NLuc (CyOFP1-NLuc-CyOFP1...the fluorescent donor for a bioluminescent donor, making BRET probes. Here the bioluminescent donor is unaffected...

...2008). It can also cross the blood-brain barrier, making it useful for applications targeting the CNS (Issa... Tabebordbar, M., Lagerborg, K. A., Stanton, A., King, E. M., Ye, S., Tellez, L., Krunnfusz, A., Tavakoli...Bertera, S., Zhang, J., Chen, C., Papworth, G., Watkins, S., Trucco, M., Robbins, P. D., Li, J., & Xiao...

...adds a repressible element to this binary system (making this a repressible binary system). The GAL80 repressor...PMID: 1319065; PMCID: PMC49329. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection of VP16... Resources on the Addgene Blog Quick guide to working with Drosophila Terminators and PolyA Signals FLEx...

... a method relies on accurate ligation of nicks lacking gaps or mismatched base pairs (as in the case for....6 (2004): 757-764. PubMed PMID: 15582400. 2. Tomkinson, Alan E., et al. "DNA ligases: structure, reaction...: 16464020.  3. Ellenberger, Tom, and Alan E. Tomkinson. "Eukaryotic DNA ligases: structural and functional...

...in common? The three most important items when looking for a house and choosing an AAV are… location, .... So how do you choose the right serotype? Keep asking questions!  Table 1: AAV Serotypes and Tropism ...choice of AAV will depend upon which section you’re looking at (striatum, hippocampus and cortex) within the...

...interest and 50 nucleotides of homology to regions flanking the target site).  The best applications for dsDNA...Daiguan Yu, Hilary M. Ellis, E-Chiang Lee, Nancy A. Jenkins, Neal G. Copeland, and Donald L. Court. An efficient...Read about Homology Directed Repair Use Knockout/Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR...

...RNase III enzyme named Dicer (Figure 1). The plant kingdom has expanded its repertoire of Dicer enzymes and...silencing effect through targeted mRNA degradation, blocking translation, or chromatin modifications of complementary...researchers studying plants and other organisms lacking this innate immunity need not worry and have benefited...

... and SciFi.  Scientists around the world began looking for the magic molecule that serves as the code ...insatiable appetite of the moviegoers for dinosaurs wreaking havoc in a theme park.  Released in 1997, GATTACA... at University of California San Diego. She is working in collaboration with Prof. Alexis Komor and together...

...the template strand. Therefore, I suggest that picking a crRNA complementary to the template strand could....For ssODN design, remember to incorporate PAM-blocking mutations (e.g. convert the NGG PAM to NGC in ...PCR genotyping (Sanger sequencing); use primers flanking the tag sequence and expect a size-shift in the...

...the better the detection of the target marker. Looking at Table 1, we can recognize a couple of fluorophores...brightness levels are often not that great. When thinking of terms like "very bright" vs. "dim" you might...investigating negative regulators of T cells and cytokine signaling in tumor immunity. When he's not in ...

...Challenge, now taking place Dec. 6, 2022! Share your AAV data, connect with other researchers working with AAV...

...community, we’re asking you for plasmid suggestions. Can’t find a plasmid you’re looking for in the repository...

...’s Day on the Addgene blog.  We’ve been mostly working remotely for the last year to give our lab and ...in the raffle, you will receive an email from us asking for your mailing address and T shirt size. You...

...of Addgene's winter holiday celebrations! We're kicking it off by sharing all the incredible guest posts...NEB's DADExperts: Matthew Norton and NEBGCE4All: Making Genetic Code Expansion AccessibleResearcher: Ryan...

...I spent about seven years working in research labs, and then pivoted to writing full-time in mid-2020...video channels are also underrated and I recommend checking them out, especially since videos on synthetic...

...better way to start off our holiday season than by thanking the twelve guest bloggers and subject matter experts...Toj, a summer intern who shared their experience working at Addgene. An Addgene Summer Internship Matteo...

...crystal structures demonstrating “the structure is strikingly similar to the design model at atomic resolution...That made it a powerful proof of concept that this kind of protein design is possible. Although Top7 was...

...culture dish and number of dishes used. Here’s to making AAV packaging a little easier! Click here to download...AAV Packaging and Transfer Plasmids Here New to Making AAV? Check out Addgene’s Step-By-Step Protocol ...

... on Twitter. As with Blugene and Aavery, we’re looking for a gender neutral name (and it can’t hurt if...is included in the final four will receive some rockin’ newly designed Addgene swag. Good luck and happy...

...decorate your lab in pipette tip snowflakes, hang stockings filled with tube rack “gifts”, or prepare for ...Here’s an example from last year’s contest:   🎶Walking in a Winter Wonderlab🎶 We decided to #DeckTheLab...

...was a year like no other, with many scientists working remotely instead of from the lab. Nonetheless, ...viruses both exhibit similar forms of symmetry. 🎶Walking in a Winter Wonderlab🎶 We decided to #DeckTheLab...

...we mentioned a few months ago, Addgene has been working with Seeding Labs to provide plasmid grants to ...Blog Learn about Plasmid Awards to Researchers Working on Rare Diseases Read About Some of Our Outreach...

..., check out my Recycling and Innovations posts. Making small changes in your daily habits can feel ineffective...wouldn’t leave your oven on all day to save time making dinner at night, so why leave your lab equipment...

...We'd like to thank Dr. Koeris for taking the time to talk to us and taking up a position on the Addgene Board...

...Looking to kick your Zoom background game to the next level? Have you been using Zoom’s default backgrounds...download the image background.     If you’re working with AAV or lentivirus in your research, you might...

...lentiCRISPR v2, you can take a look at the protocol kindly provided by the Zhang lab. Even though lentiCRISPR...around the world. We thank our many depositors for making these reagents available to the research community...

...Their muscles showed an increase in inflammatory cytokines as a result of those mild stressors as usual, ...use a good run right about now. And, if you are looking to get your hands on PGC-1a for studies in your...

...Structural details All this, and scientists are still working out important details in their understanding of...basically happened in a year. It’s incredible.” Looking for CRISPR Plasmids? CRISPR-Cas9 Plasmids available...

...evolved tRNA adenosine deaminase that is fused to a nicking Cas9. This base editor can be used for A•T to G...Valcyrie is a CRISPR-based marker excision method for marking distinct cell clones developed by the Michalis ...

...Konermann said she is already aware of research groups making use of the new optogenetic tools in C. elegans ...neurons, she offers a tip: save yourself some time by making an AAV1 supernatant. (The details can be found ...

... Environmental Protection Agency, which started making regulations such as preventing manufacturers from...distribute it to industrial and residential markets. Rethinking our approach to lab waste is something we all...

...Balance - Vini describes how she balances working in the lab and working with SITN. She points to collaboration...

...Plasmid pumpkins, team costumes, and spooky science stories. Yes, please! At Addgene, we love finding...addition to the Halloween contest, we’ve also carved pumpkins (yes, Blugene made an appearance there too) and...

...gRNAs can save you the time that would be spent making and testing entirely new gRNA designs. You can ... filter the table and find exactly what you're looking for. Search using the box highlighted in red above...

...for all to enjoy. The largest competition of this kind is Princeton University’s Annual Art in Science ...Time magazine . Other smaller competitions are now taking place and gathering momentum, including in Cambridge...

...fluorescent dCas9-KRAB and dCas9-VPR for quantifying and tracking cells that express both the dCas9 construct and... by co-delivering TET1 and protein factors for linking methylcytosine oxidation to DNA repair, which increases...

...pSiM24, can also be used with biolistic methods, making it useful for engineering plants that are not susceptible...available pCAMBIA and pKYLX vectors, respectively, making it ideal for large gene transfer. The M24 promoter...

... rather than complete recombination. If you’re looking to use site-specific recombination in Drosophila...techniques. Semprini S, Troup TJ, Kotelevtseva N, King K, Davis JR, Mullins LJ, Chapman KE, Dunbar DR, ...

...coincidentally speak the same Polish sub-dialect making their Skype communications extra fun) who shared...to Norton Antivirus. Antivirus programs work by taking info from the code of viruses to give information...