We narrowed to 612 results for: SCR
-
TypeBlog PostPublishedFeb. 14, 2019, 2:35 p.m....PMC5517107. Additional resources on the Addgene blog Subscribe to the Addgene blog Find lab tips for your research...
-
Antibodies 101: Affinity Tags
TypeBlog PostPublishedSept. 19, 2023, 1 p.m....protein for downstream applications Below, we describe a few of the most common tags available. Maltose... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog PostPublishedSept. 12, 2023, 1:15 p.m....repository for Cas enzymes. Open repository of CRISPR screens Cellosaurus: A knowledge resource for cell lines... -
Antibodies 101: Fc Effector Functions
TypeBlog PostPublishedMay 18, 2023, 1:15 p.m.... 2022). So the next time you see an antibody description with the Fc domain as only providing structure... -
We're Updating Our Hold for Publication Policy
TypeBlog PostPublishedJune 24, 2025, 1:15 p.m.... release plasmids on hold if they are clearly described in a journal publication. Plasmids will be released... -
Oh, The Places You Can Go: Careers in Science Communication - Product Development
TypeBlog PostPublishedOct. 18, 2017, 1 p.m....a science education company. And make sure to subscribe to the Career Channel of the blog so you don’t... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog PostPublishedOct. 3, 2017, 12:11 p.m....in future career blog posts. And make sure to subscribe to the Career Channel of the blog so you don’t... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog PostPublishedJune 30, 2015, 2 p.m....primary cells. In 2009, Zsuzsanna Izsvak’s lab screened many SB tranposase variants for hyperactivity ... -
Management for Scientists: Giving Feedback
TypeBlog PostPublishedFeb. 6, 2015, 2:46 p.m....a 5 part series on Management for Scientists. Subscribe to the Addgene Blog to follow the entire series... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog PostPublishedJan. 30, 2014, 3:29 p.m....Plasmids 101 Posts Learn How to Use Blue White Screening to Simplify Your Cloning Experiments See Where... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m....to select against CRISPR positive plants, but screening these can be challenging and requires specialized... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog PostPublishedMay 25, 2021, 2:29 p.m.... advances in gene synthesis, affinity reagent screening, and next-generation sequencing of hybridomas ... -
Plasmids 101: Origin of Replication
TypeBlog PostPublishedNov. 11, 2020, 2:23 p.m....p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300-500 ColE1 (derivative) and F1** A Relaxed... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m..... “They took a dividing yeast and did a genetic screen to identify the factors that regulate cell division... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m.... for control elements such as promoters and transcription terminators, the boundaries are less obvious... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog PostPublishedMarch 9, 2017, 3:40 p.m....frustrating for solo flyers Other people will routinely scrutinize your data—science can be surprisingly more rigorous... -
Using Addgene's New Viral Service for Your Research
TypeBlog PostPublishedNov. 17, 2016, 3:30 p.m....modulate its expression, or perform a genome-wide screen, we’ve got viruses to help you out. Of course, ... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog PostPublishedSept. 21, 2023, 1:15 p.m....immunocytochemistry (ICC). Not all of the solutions described here may be as effective for other techniques,... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog PostPublishedAug. 31, 2023, 1 p.m....using NeuroStack and the fibers as previously described, then measured electrophysiological potential ... -
What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m....with kidney cells (Shaw et al.). Since then, transcriptome studies of the cell line have determined it ... -
New Viral Vectors - Winter 2025
TypeBlog PostPublishedJan. 28, 2025, noon...delightful. Grab a hot beverage and a blanket, then scroll on for some wintery delights! Table 1: New... -
Viral Vectors 101: Transductions
TypeBlog PostPublishedApril 30, 2024, 1:15 p.m....enters a host cell, enters the host nucleus, is transcribed into RNA, and is translated into protein. ... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....rRNA in bacterial RNA-Seq. This enriches for transcripts of interest without an increase in the number... -
Plasmids 101: TOPO Cloning
TypeBlog PostPublishedOct. 27, 2016, 2:30 p.m....cassette allowing a researcher to perform blue-white screening after transformation - self joining of the vector... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....must be expressed from a plasmid or in vitro transcribed, and changes in gRNA secondary structure can ... -
Plasmid Cloning by PCR
TypeBlog PostPublishedMarch 29, 2016, 2:30 p.m....the downstream steps. For this example, we will describe how to copy a cDNA from one vector into a new ... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m.... own experiences founding and running miLab to describe some key lessons I learned while becoming a health... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....you particularly good at writing and editing manuscripts? Are you interested in scientific policy? Next... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog PostPublishedApril 9, 2020, 1:15 p.m....s time, we’ve published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction... -
25 Work From Home Tips for Scientists
TypeBlog PostPublishedMarch 19, 2020, 1:15 p.m....visualizing your data, such as R or Python. Write a manuscript. If you have all the data you need to begin writing... -
Creating accessible biology activities in schools with BioBits
TypeBlog PostPublishedAug. 2, 2018, 12:56 p.m....produce that green fluorescent protein through transcription and translation. Unfortunately, having live ... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog PostPublishedFeb. 20, 2018, 2:30 p.m....of the workshop, NEB gave a brief presentation describing the technology. Then we split into pairs to actually... -
Visualizing Translation at the Single Molecule Level
TypeBlog PostPublishedAug. 1, 2017, 1:15 p.m....cell cycle protein Emi1, showing that ~80% of transcripts with a long 5’ UTR were translationally silent... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog PostPublishedJune 20, 2017, 2:30 p.m....shipment processes. After reading a publication describing how short term storage on dry ice can shift sample... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog PostPublishedApril 19, 2017, 3:25 p.m....all over the world. The rest of this piece will describe plexWell™ in more detail, and how we are using... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog PostPublishedJune 4, 2019, 1:02 p.m....site-saturation mutagenesis, and medium-throughput screening, she increased the affinity of the sensor for ... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog PostPublishedMarch 14, 2019, 1:54 p.m....producer cells and purification protocols often describe a process that begins with cell lysis to release... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog PostPublishedOct. 19, 2023, 1:15 p.m....region of an ITR with a sequence containing transcription factor binding sites can increase transgene ... -
K. phaffii: Rising to the Occasion
TypeBlog PostPublishedApril 4, 2023, 1 p.m....Organisms Plasmids 101: Yeast Vectors Tips for Screening with the Yeast Two Hybrid System Additional... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog PostPublishedNov. 7, 2019, 2:18 p.m.... LMB in Cambridge UK and then a postdoc at The Scripps Research Institute in La Jolla, California. She... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog PostPublishedMarch 24, 2016, 2:30 p.m....relatively modest, requiring expressed Cas9 protein, transcribed sgRNA, and optional donor DNA. Chris Richardson... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m....points. At Addgene, we like to do this live on the screen using a Google Doc. Action items: Before leaving... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog PostPublishedOct. 9, 2014, 3 p.m....has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer comparing a standard... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog PostPublishedAug. 28, 2014, 3:34 p.m....time-intensive, so we recommend a variety of ways to screen and verify your plasmids. Here, we'll cover restriction... -
Hot Plasmids - May 2022
TypeBlog PostPublishedMay 26, 2022, 1:15 p.m....expression of the fluorescent protein by a specific transcription response element (TRE) or kinase translocation... -
AAV Q&A with Tim Miles
TypeBlog PostPublishedMay 17, 2022, 1:15 p.m....Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors Blog Posts ... -
Antibodies 101: ChIP
TypeBlog PostPublishedMarch 10, 2022, 3:25 p.m....your protein of interest is a low abundance transcription factor or cofactor. In such cases, the gentler... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog PostPublishedFeb. 2, 2021, 2:15 p.m....consider the advice below: 1. Eligibility check Scrutinize every opportunity prior to starting an application... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....next-gen sequencing (NGS), and high-throughput screening. While some developers had science backgrounds... -
New and Upcoming Viral Vectors - May 2020
TypeBlog PostPublishedMay 28, 2020, 1:15 p.m.... genes and is used for genome-wide inhibition screening. Find the Dolcetto lentiviral prep here Control...