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Showing: 181 - 200 of 565 results
  1. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...different aspects of manufacturing and quality, especially with regard to compliance with health authority...cancer cells under drug treatment.” Stegmeier especially finds the value in the wealth of resources on...
  2. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ... is positively regulated by RNA or proteins, respectively. A plasmid's copy number has to do with the ... contribute as well. These considerations are especially useful to keep in mind if you are planning to...
  3. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...Since the start of this crisis, we’ve been very responsive to the mood of the Addgenies — and they were ...said CZI Head of Science, Cori Bargmann. "CZI is especially proud to support Addgene now, when their distribution...
  4. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ... time, the use of episome seemed appropriate, especially since the Fertility, or F-factor discovered by...scientific community. Prior to this, bacteriophage, especially lambda, was the tool of choice for molecular ...
  5. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...explore the functional differences among mutations responsible for the disease known as Alternating Hemiplegia...disorder.” In 2012, researchers identified the gene responsible for the disease as ATP1A3. The gene encodes a...
  6. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...equivalent of a “chemistry kit” for topics in biology, especially those at the molecular and cellular level. The... of this is conducive to a classroom setting, especially for a school with a limited budget. While there...
  7. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...classify unknown, or ‘orphan’, TFs into their respective families. Given the number of poorly studied ...cellular fates were then matched with candidate TFs responsible for that differentiation.   Fig. 2: Single...
  8. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...by glutamate, and tweaking it to improve its responsiveness, they showed that iGluSnFR could detect neuronal...sensitive and better localized for synaptic glutamate response than its predecessors. The detailed characterization...
  9. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ... can and do play this role, and we take our responsibility to push for clear communication of science ...broader role as Vice President. Her current responsibilities encompass overall leadership of the Cell Press...
  10. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...specific parameters: jGCaMP7s is the most sensitive responder to action potentials. For a single action potential...brighter than GCaMP6s. jGCaMP7f is the fastest responding variant. jGCaMP7b exhibits the brightest resting...
  11. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...they provide rapid access to validated reagents, especially during a time when these materials may not be...of K18-hACE2 Mice Infected with Severe Acute Respiratory Syndrome Coronavirus. JVI 81:813–821 . https:...
  12. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...using CRISPR/Cas9. They are targeting the genes responsible for both early onset and late onset AD. In this...ability to influence editing efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists...
  13. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...is a variant with increased PAM flexibiliy and eSpCas9/SpCas9-HF1 have increased targeting specificity...CRISPR/Cas systems have a variety of enzymes responsible for processing foreign DNA as well as the RNA...
  14. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...rate than that of bacteria (~24 h vs 20 min, respectively). Therefore, it is not always mission critical...plasmids containing the viral EBV or SV40 ORIs, respectively. The presence of these viral components greatly...
Showing: 181 - 200 of 565 results