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We narrowed to 405 results for: Lif;

Showing: 181 - 200 of 405 results

  2. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...two-step staining with antibodies. Nanobodies also simplify multicolor staining experiments because they don...epitope-mapping and native protein complex isolation. eLife. PubMed PMID: 26633879 PubMed Central PMCID: PMC4755751...

  3. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...park, part time work, bills, (at times) a social life and rest. I need it just as much in my current role... team. All while still paying bills and having a life outside my job. The same can be said about teamwork...

  4. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...- they relieved some of the pressure of start up life and maintained their sanity by throwing fun into...      Additional Resources Read other posts about life at Addgene!Read more of our careers posts!    ...

  5. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    Published
    Sept. 9, 2025, 1:15 p.m.
    ...to understanding some of the basic principles of life and health and works to advance foundational research...communication with gene promoters. Cellular and Molecular Life Sciences, 78(19–20), 6453–6485. https://doi.org/...

  6. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...experimental purposes. But what now? MoClo really simplifies vector construction by combining restriction ...process to produce a multigene construct. Figure exemplifies the extraction and use of a coding sequence of...

  7. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...staining. Direct ELISAs also lack the signal amplification afforded by secondary antibodies and thus are... bind to a single primary antibody and thereby amplify the signal. Since the primary antibody does not...

  8. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...to that of a professional email. Notes from real-life hiring managers In preparation for updating this...that stage, cover letters can be a barrier for qualified applicants. In short, while a cover letter can...

  9. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...photostable than GFP. In addition, antibody-based amplification of their signals is weak and increases background...sites for primary antibodies which can be used to amplify the direct smFP signal within sub-cellular structures...

  11. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...University and became a lecturer in the College of Life Sciences that same year. Her research is focused...studying biochemistry at the University of Southern California. He is also an intern at the Yang Lab of the ...

  12. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...knowledge of the software, sharing knowledge allows for life to happen without impacting the overall work. Each...and tidy it up. Conclusion While none of us are life science majors, we do our best to understand the...

  13. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...two other areas that could potentially improve the life expectancy of mesothelioma patients: early detection...use, they will have a much greater impact on the life expectancy and overall survival of mesothelioma ...

  14. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...possible, especially if the infection is serious or life-threatening, treatment will often begin before this...development of resistance; it’s a natural process of life and one which has allowed bacteria to evolve and...

  15. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...expertise in science careers, women in STEM, work-life negotiation, and mentorship, we decided to have ...questions about science careers, women in STEM, work-life negotiation, and mentorship. We can direct people...

  16. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...base pair sequence surrounding your gRNA. Once amplified, you can insert this region into a pCAG-EGXXFP...to the day you have your F0 generation (barring life’s little accidents and stuff ups). This is a huge...

  17. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...acceptor photobleaching method and the fluorescence lifetime imaging microscopy (FLIM) method. Acceptor photobleaching...not to destroy the donor molecule. Fluorescence lifetime imaging microscopy method FLIM has been developed...

  18. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...involving genomic screens, the data stream is so prolific that raw information is made available to everyone...types? And how long should data be archived? Simplifying data access Some of the above mentioned issues...

  19. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ... by a ribonuclease called Dicer. In a great simplification of the process, Dicer chops off the loop of...10.1038/35888  Iwakawa, H., & Tomari, Y. (2021). Life of RISC: Formation, action, and degradation of RNA-induced...

  20. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...figure out all the ways a given ELN can make your lab life easier. If you can talk to an ELN support team directly...more tools for reproducibility 5 great apps for lab life Developing Lab Management Software for Biology ...
Showing: 181 - 200 of 405 results