We narrowed to 316 results for: Mut
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TypeBlog PostPublishedMarch 21, 2017, 2:30 p.m....active area of research due to the possibility that mutation or recombination with human retroviruses could...Efficient gene transfer Potential for insertional mutagenesis: Replication-incompetent lentiviruses with human...
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9 tips for a successful postdoctoral experience
TypeBlog PostPublishedOct. 9, 2018, 12:21 p.m....with senior investigators. Discussions can reveal mutual scientific interests, complementary perspectives...research to search committees, and can lead to mutually beneficial collaborations. 7. You cannot get too... -
Important Considerations When Using AAVs
TypeBlog PostPublishedJune 13, 2017, 2:30 p.m....them. There are suspected cases of insertional mutagenesis in humans (1) and AAVs are non-enveloped viruses... Russell, David W. "AAV vectors, insertional mutagenesis, and cancer." Molecular Therapy 15.10 (2007):... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog PostPublishedApril 16, 2024, 1:15 p.m.... most often consist of an enzyme that has been mutated to block its catalytic activity and instead become... later work showed that introducing a Leu28Cys mutation in eDHFR does lead to covalent binding of the ... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m.... into question. Specifically, CRISPR-generated mutants believed to be knockouts can maintain partial function...process is tedious and time consuming, as RNAi mutants with a reduced growth phenotype will require additional... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog PostPublishedMarch 21, 2024, 1:15 p.m....especially for G-protein-coupled receptors (GPCRs). Mutations in receptor sites can affect the fluorescence ....org/10.1038/s41592-021-01338-6 Grimm, J. B., Muthusamy, A. K., Liang, Y., Brown, T. A., Lemon, W. C.,... -
Imaging Tools and Following a Passion for Basic Biology: Interview with Joachim Goεdhart
TypeBlog PostPublishedMarch 9, 2018, 2 p.m....creation of improved fluorescent proteins through mutagenesis. Chapter 6 - The future of FRET and fluorescent... -
New Podcast Segment: Hot Plasmids
TypeBlog PostPublishedMay 17, 2017, 3:37 p.m.... new tool for making diverse, localized point mutations. Listen to Our Segment on CRISPR X Hot Plasmid... -
Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More
TypeBlog PostPublishedNov. 15, 2016, 3:30 p.m.... focusing on safety and the ability to generate mutant microbial strains faster than ever before. 29:54... -
CRISPR Cheat Sheet
TypeBlog PostPublishedJan. 27, 2021, 2 p.m....multiple genes in a cell population. The resulting mutant cells are screened for phenotypes of interest. ... -
New Viral Vectors - Summer 2024
TypeBlog PostPublishedJuly 9, 2024, 1:15 p.m....viral service with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV9 Biosensors (Control) Li New viral service... -
What's New in CRISPR - May 2019
TypeBlog PostPublishedMay 28, 2019, 12:49 p.m..... Thus, they engineered two CBE variants with mutations in the rat APOBEC1 that decreases the number of... -
What's New in CRISPR - Spring 2019
TypeBlog PostPublishedMarch 21, 2019, 12:47 p.m....DNA strand. The lab then found gain-of-function mutations that allows it to be repurposed for double strand... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog PostPublishedNov. 29, 2018, 1:53 p.m....infectious organisms (ex: SARS-CoV-2) and genetic mutations. By providing a gRNA specific of a viral sequences... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog PostPublishedAug. 7, 2017, 1:58 p.m.... protein’s structure and also discovered many mutations that alter and improve its function and shift ... -
What's New in CRISPR - December 2019
TypeBlog PostPublishedDec. 17, 2019, 2:37 p.m....Single base editor that induces C-to-T and A-to-G mutations Nozomu Yachie’s lab recently developed a base ... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog PostPublishedMarch 22, 2016, 2:30 p.m....pathways, they identified a set of one to three mutant complementary DNAs (cDNAs) representing core nodes... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog PostPublishedOct. 31, 2019, 1:06 p.m....discovered in Drosophila melanogaster (Gilbert, 2004). Mutations in these genes are lethal, associated with defective... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog PostPublishedDec. 12, 2023, 2:15 p.m....adrenergic receptor; nLightG contains circularly permuted green fluorescent protein (GFP), and nLightR uses... -
What's New in CRISPR - September 2019
TypeBlog PostPublishedSept. 10, 2019, 12:56 p.m....activation and genome editing by fusing Cas9 to a mutated human estrogen receptor. This protein fusion stays...