We narrowed to 221 results for: PORA

1. Deciphering the Mysteries of Behavior with Viral Vectors

   Type

   Blog Post

   Published

   Sept. 17, 2019, 12:40 p.m.

   ...of viral vectors to fine tune gene expression temporally and spatially, exciting times are ahead for behavioral...

2. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

   Type

   Blog Post

   Published

   Aug. 13, 2019, 12:58 p.m.

   ...PMCID: PMC6247916. Felker, A., and C. Mosimann. "Contemporary zebrafish transgenesis with Tol2 and application...

3. Save Time with Transient Plant Leaf Transformations

   Type

   Blog Post

   Published

   July 25, 2019, 12:03 p.m.

   ...protein half-lives then GUS and are therefore good temporal reporters to measure promoter activity, for example...

4. New Tools Enable CRISPRa for Neuroscience Applications

   Type

   Blog Post

   Published

   July 2, 2019, 12:51 p.m.

   ...of this construct is Cre-dependent, enabling spatiotemporal control over its expression. Constitutive or...

5. Antibodies 101: The Four ELISAs and When to Use Them

   Type

   Blog Post

   Published

   July 25, 2024, 1:15 p.m.

   ...antibody and a conjugated secondary antibody. Incorporating a secondary antibody increases the sensitivity...

6. Viral Vectors 101: Preparing Pooled Libraries

   Type

   Blog Post

   Published

   July 13, 2023, 1:15 p.m.

   ...this guide, we hope you feel more confident incorporating them into your research. And, of course, if ...

7. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

   Type

   Blog Post

   Published

   Jan. 24, 2023, 2:15 p.m.

   ... penetration depth as well as high spatial and temporal resolution, making it ideal for visualizing gene...

8. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

   Type

   Blog Post

   Published

   Oct. 13, 2022, 1:45 p.m.

   ... particular gene isn’t feasible. For example, temporary repression or knockdown allow you to study essential...

9. Plasmids 101: Control Plasmids

   Type

   Blog Post

   Published

   April 9, 2015, 3:29 p.m.

   ...Figure 3), we can continue with our experiment, incorporating additional positive and negative controls, performing...

10. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type

    Blog Post

    Published

    May 5, 2020, 1:15 p.m.

    ...results in fluorescence and this approach can be incorporated into a lateral-flow readout slip that allows...

11. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    Published

    Jan. 4, 2022, 2:15 p.m.

    ...That can mean a willingness to make yourself temporarily uncomfortable for the sake of a common good. ...

12. Filming Science Videos in the Age of Social Distancing

    Type

    Blog Post

    Published

    May 20, 2021, 1:15 p.m.

    ...things return to relative normalcy, I’ll be incorporating that mobile monitor stand into every shoot. ...

13. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type

    Blog Post

    Published

    May 13, 2025, 1:15 p.m.

    ...the resulting double-stranded DNA transgene is incorporated into a large complex of both viral and cellular...

14. Antibodies 101: Beyond Surface Labeling

    Type

    Blog Post

    Published

    June 27, 2024, 1:15 p.m.

    ...bromodeoxyuridine (BrdU) can be used. These dyes are incorporated into the DNA during DNA synthesis and then diluted...

15. Fluorescent Proteins 101: When GFP lets you down

    Type

    Blog Post

    Published

    Aug. 23, 2018, 12:05 p.m.

    ...non-natural fluorescent amino acids and their incorporation into proteins are steps in that direction (Chatterjee...

16. Adeno-associated Viruses (AAVs) for Genome Editing

    Type

    Blog Post

    Published

    March 27, 2018, 1:32 p.m.

    ...without a nuclease Although the mechanism of incorporation is not well understood, researchers have been...

17. Hot Biosensors 2022: Year-End Roundup

    Type

    Blog Post

    Published

    Dec. 6, 2022, 2:15 p.m.

    ...plasmids here!   Farahani PE, et al. pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling...

18. Plasmids 101: Biotinylation

    Type

    Blog Post

    Published

    Nov. 15, 2018, 1:50 p.m.

    ...biotinylated primer. Biotin can also be enzymatically incorporated during PCR using a biotin labelled dUTP. There...

19. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    Published

    Sept. 14, 2016, 2:30 p.m.

    ...found in your gene of interest) and prevent incorporation of extensive sequences used for cloning into...

20. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    Published

    March 1, 2016, 3:30 p.m.

    ...primers for PCR amplification of fragments to incorporate such regions of homology. This video gives a ...