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We narrowed to 468 results for: SEC

Showing: 181 - 200 of 468 results

  1. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...summer of 2014 (after extensive discussions with biosecurity experts, ecologists, and national authorities... Holm, D. G. & Suzuki, D. T. Genetic Control of Insect Populations: I. Cage Studies of Chromosome Replacement...

  2. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ... pC5Kan-P2A P2A Insect pUltra P2A and T2A Lentiviral Ac5-STABLE2-neo T2A Insect   For the co-expression...

  3. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own proteins, making the purification...production of Fab fragments where variable chains are secreted into the periplasmic space to be oxidized and ...

  4. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...plants, microbes, blood or mammalian cells in 30 seconds or less without using a pipette.  Here’s how this... from plants, animals and microbes in under 30 seconds. PLoS biology. https://doi.org/10.1371/journal....

  5. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...topic. For example, it may be useful to adapt the second version (multiple presenter, figure by figure) ... seemed to be of use, it’s worth scheduling the second and other subsequent meetings. The number of participants...

  6. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...to say “no, that’s not in my wheelhouse”, take a second to reflect. For example, say your boss approaches...Maybe you also showed critical thinking skills by dissecting their problem and piecing together a really great...

  7. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...necessary to create a replication-competent virus. Second, Tat is eliminated through the addition of a chimeric...us know how you use lentivirus in the comments section below! References 1. In vivo gene delivery and...

  8. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...-like endonuclease domain, but they lack Cas9’s second HNH endonuclease domain. Cpf1 cleaves DNA in a ...to cleave again and possibly mediate HDR. This “second chance” mechanism might improve the frequency of...

  9. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...is transferred to a 2D barcoded tube (see next section) and every time something is removed from this ...deposit. Using barcoded tubes is part of Addgene’s secret to success. It helps us quickly process samples...

  10. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...results of any experiment I’ve run in under thirty seconds.” That’s exactly what you would want to talk about...possible, send it to a mentor or colleague for a second look and helpful feedback!  What if I’m not a good...

  11. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...shared server. The blue box labeled “A5” on the second rack in the corner freezer sounds easy to remember... some things I could pay attention to, so my dissections go smoother next time?” or more generally “How...

  12. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...expression to appreciable levels. We wanted to create second-generation mARGs with higher expression, a smaller... structural gene gvpA marked with EBFP2 and the second cassette contains the P2A-linked accessory genes...

  13. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...2020). Further, this group also characterized a second Cas9 variant, SpRY, which cuts at NRN PAMs with...double strand break.   Prime editors are the second generation of base modifiers available. This system...

  14. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ... date with their third party libraries and any security updates. Most of the time, updating these libraries... which means there will be no further updates, security or otherwise. This might sound like Greek to you...

  15. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ... google “how to write a scientific review.” The second thing I did was stare in horror at the limited ...paper. I also learned to appreciate the discussion sections of the primary literature as a resource that both...

  16. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...include surgery to remove cancerous masses (tumor resection), systemic chemotherapy to kill cancerous cells...added to the treatment recommendations for non-resectable mesothelioma by the National Comprehensive Cancer...

  17. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ... approach can lead to new directions, while the second approach could terminate with the rejection of ... His interests include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization...

  18. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...non-optimal codons are associated with specific secondary structures in 10 closely related yeast strains...sequences Restriction sites Sequences prone to create secondary structures in RNA transcripts Effects on transcription...

  19. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...quantifying AAV: qPCR efficiency can be affected by second-order structure of the AAV genome, caused by repeats...2010). In addition to the AAV RSM, we include a second AAV reference sample of known titer, which is at...

  20. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...keeping in touch by email and social media (see the section on “Maintaining Relationships” below). Expand: ...learning and, in my experience, mistakes and insecurities are more easily excused. When you reach out ...
Showing: 181 - 200 of 468 results