... SNAPf. eDHFR: The Virginia Cornish and Michael Sheetz labs demonstrated in 2005 that proteins fused with...j.chembiol.2019.01.003 Gallagher, S. S., Sable, J. E., Sheetz, M. P., & Cornish, V. W. (2009). An In Vivo Covalent...doi.org/10.1021/cb800025k Miller, L. W., Cai, Y., Sheetz, M. P., & Cornish, V. W. (2005). In vivo protein...10.1038/s41467-023-40788-8 Ulrichs, H., Gaska, I., & Shekhar, S. (2023). Multicomponent regulation of actin ...

...genes across the genome. Although this tool was published in 2011, it was only recently made available through...outer mitochondrial membrane. Sequestration is accomplished by knocking down your endogenous protein of ...shRNA. This system requires less work than using established methods and minimizes some of the technical issues...the CRUSH vector in transgenic mice, the lab established a dual-color RNAi “sensor” mouse strain in which...and involve many steps. The Drew Endy and Keith Shearwin labs have developed a new, streamlined approach...

...Zhang’s lab recently developed a platform called SHERLOCK (specific high-sensitivity enzymatic reporter ...more. Read the paper in Science Find the new SHERLOCK plasmids More precision with dual-nuclease Cas9...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...

...world. 4)      The Annals of Internal Medicine published systematic reviews on an agent used to promote... into account. 7)     Science’s John Bohannon published an article describing a sting operation in which...submitted a phony paper to a list of 304 open access publishers. By the time the magazine went to press, no less...

... LaManna, published Mar 8, 2016. The updated and expanded version by Nyla Naim was published Sept 3, 2020...dCas9 Initial photoactivatable CRISPR systems published in early 2015 focused on using light to control... and repeatable. Since these initial studies published in 2015, more split systems have been developed...

...listed in the table has been used effectively in a published article. This blog post will serve as a guide ...deposited gRNA plasmids or from submission of a spreadsheet containing the validated gRNA sequences alone... and we’ll add them to this list. Access the spreadsheet for submitting your gRNA sequences here. Features...

...better for you) and place them on an easy to reach shelf. Make sure all notebooks are visibly labeled on ...you’re looking for. Tip 3: Share protocols and spreadsheets with lab members Now that you’ve organized your... may also want to consider moving your paper spreadsheets for reagent storage locations, such as the list...

...microbial DNA from groundwater. Now, they’ve recently published data demonstrating that CasX is active in both...for cancer research The Sidi Chen lab recently published two CRISPR knockout libraries for cancer research...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...

...Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems...E. coli and T. citrea scarless editing plasmids Sheng Yang’s lab describes a two-plasmid system that combines...the above CRISPRi section, the Marraffini Lab published a robust tool for CRISPRi and CRISPRa using RNAP-Omega-dCas9...systems is base editing. This process is generally accomplished by fusing an enzyme capable of converting one...

...Coroadinha, 2006) and retroviral production systems (Shen, 2011). To our knowledge the effect of hyperosmolarity...37.10 (1991): 989-993. PubMed PMID: 18597326. 5. Shen, Chun Fang, and Amine Kamen. "Hyperosmotic pressure...22154564. 6. Stoneham, Charlotte A., Michael Hollinshead, and Amin Hajitou. "Clathrin-mediated endocytosis...

...editing and prime editing approaches. Recently, Xue Sherry Gao’s lab at Rice University has developed DAP ...tRNAs Luckily, a fun feature of tRNA was fully unleashed by the Gao Lab’s work. Led by PhD student Qichen... Qichen Yuan is a PhD student in the lab of Xue Sherry Gao at Rice University.    References and Resources...

... is universally useful. 4. Chemical Safety Data Sheets by ThatsMyStapler Safety always comes first with...including this MSDS app. The Chemical Safety Data Sheets app has a search function that allows the user ...Restriction Enzyme Tool by Promega Material Safety Data Sheet by RSS-ICTD Amino Acid Structure Quiz/Card by Andrey...

...Imaging Improving Well-established High-throughput assays Improving Well-established Biological sources...describing the cloning of firefly luciferase was published in 1985, several studies utilized luciferase as...

...and joined each fragment with one part of an established pair of proteins (iLID and SspB) that undergo.... PubMed PMID: 29300010. miRNA reporter vectors shed new light on mechanisms of miRNA-mediated gene regulation... to be fully elucidated. Ron Weiss’s lab at MIT pushed the field further with the creation of the largest...

...Sonication provides a truly random digestion, but is harsher and requires more optimization. Enzymatic digestion...more of the protein:DNA complexes. Once you’ve finished fragmenting the DNA, spin down the samples to ... Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay, let’s do a quick ...

... question. Three different labs have recently published work that provides a solution to this problem....genomic deletions In PRIME-del, developed by Jay Shendure’s Lab, paired pegRNAs work with the PE2 enzyme ...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...

... that can be referenced. In addition the data published in microPublications is curated and added to other...but there are Drosophila and zebrafish articles published there as well.   Worm Development Dynamics Database...used for introducing point mutations that was published in a microPublication (Schwartz and Jorgensen,...

...Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some enzymatic fusions... is a graduate student in the laboratory of Dr. Moshe Szyf at McGill University, working to develop CRISPR-based...Feinberg A, Vogelstein B (1983) Hypomethylation distinguishes genes of some human cancers from their normal...

... an increase in the complexity. In 2018, they published a paper profiling four base overhang ligation ... to generate high-fidelity reactions. (Need a refresher? The five rules are: (1) don’t use the same overhang...fragments may be near the upper limit of what can be accomplished with Golden Gate Assembly—even when getting ...

...those tips for you in one place! First, a quick refresher: prime editing is a “search and replace” genome...j.cell.2021.09.018 Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, ...Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & ...