Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 340 results for: abo.4

Showing: 181 - 200 of 340 results

  1. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...distributed – have changed so dramatically in the past 4 years. I kept busy for the whole poster session, talking...It's been about 14 years since I last attended a Keystone Meeting – far too long. Holding these meetings...CRISPR technology in just 2 years and excitement about how much more there is left to do with the characterization...downstream output. Tuesday morning's talks were also about circuitry and synthetic biology, and I found myself...plasmid sharing to those who were anxious to hear about what was coming next. I also got to show off our... updated Synbio page to people who were curious about these newest features of our website.  I want to...

  2. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ... and technology for bioprocesses (1989): 272-278. 4. Ozturk, Sadettin S., and Bernhard O. Palsson. "Effect...resources on Addgene.org Learn about Addgene's viral service Learn about viral production Find viral protocols... By now you have probably heard about Addgene’s viral service but did you know that Addgene also performs... approach can be adapted by vector cores and laboratories alike as an easy and inexpensive method to improve...Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and ...

  3. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...fields not solely the specific area the writer works.  4. Practice writing everyday Overall, the most important...successful writer. When I originally talked to my PI about becoming a better writer, I was unsure if I should... a manuscript and critiquing it, were not too laborious, but helped build my understanding of how to generate...and the writing workshop. SWPI's writing workshop About a month later I received another email notifying... manuscript, scientists have a tendency to be elaborate and over explain the material. The point of writing...

  4. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ... you can use to express your protein of interest. 4. Plasmids 101: Blue-White Screening Getting a lot ...eyes open for any new technologies that will bring about the next revolution in biological research. We’re...Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need for your next ...Addgene Browse Our CRISPR Resources Learn More about Viral Vectors Browse the Repository  ...

  5. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...cytotoxicity against tumor targets. Nat Med. 2000 Apr;6(4):443-6. doi: 10.1038/74704. PMID: 10742152. Dunkelberger...phagocytosis.  Other functions We’re still learning about Fc effector functions and all that antibodies can...isotypes and Fc receptors that we don’t know much about yet. Who knows what kinds of functions they may ...

  6. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...be collected from heterozygous individuals. Only 1/4 of the descendants of the heterozygous plants, therefore... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...resistant disease. (RUBY-red: You can’t beet it!) About the Author  Andrew Willoughby is a graduate student...

  8. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...for individual cells. Each cell is labeled with 3-4 tags to increase the diversity of the CellTag library...Additional resources on the Addgene blog Learn more about induced pluripotent stems cells (iPSCS) and reprogramming...

  10. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...within a few hundred nanometers of the coverslip (4). Essentially, an optical section is achieved that...biophysics 48, 178-243 (2015). PubMed PMID: 25998828. 4. Axelrod D. Total internal reflection fluorescence...call Epi-fluorescence microscopes. (See left image above for an example image). Point Scanning Confocal Microscopy... plane to reach the detector and all light from above or below is excluded. Parallelized Confocal Microscopy...due to limitations in light penetration mentioned above (5). Thick static samples Ex: Fixed tissue sections...sectioning. Point scanning confocal microscopes (see above) often provide the highest quality images for this...

  11. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...Cre-mediated marker excision, it takes approximately 3-4 weeks to obtain a homozygous, outcrossed knock-in ...another similar co-conversion marker (repair of a zen-4(cle10ts) allele), increasing experimental options....learn more about genome editing with CRISPRs? Check-out Addgene's CRISPR Resources Learn about gRNA design...interest, I encountered low efficiency (0.13%) and laborious handling (screening 768 F1 animals). Recovering...

  12. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...become your article title.       Figure 4: Add plasmids to the article by clicking “Add Plasmid... cloning information for the gene or insert. Step 4: Growth and Distribution Please indicate which strain...annotate on this page. Figure 7: Enter information about the gene/insert(s). Step 3: Cloning Information ...

  13. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...grant writing Job changes can happen regularly (every 4-6 years is common), but this can also bring new opportunities—projects...students Collaborations may be less “complicated” - for example, easy to initiate a collaboration without...up the pace Constant worry about grants and renewals What is different about non-academia? Scientific...make a decision about something like this without sufficient data? Starting to learn about the diverse options...with scientists just like you What is different about academia? Publishing in peer reviewed journals ...without having to worry about proprietary information Work hours are long, but there is more flexibility with...paths to pursue. Spend some time really thinking about your motivations and what parts of being a scientist...

  14. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...your role as a lab leader, and then on to Chapter 4 for advice on staffing your lab. Let us know if you...start their own academic research laboratory. How does one even go about starting a lab? Obviously, funding...always helps. But how does one build a strong collaborative research team motivated by similar visions and...booklet. What has been the most surprising thing about becoming a PI? Tom Ellis has a one word answer: ... has to deal with. My inbox currently stands at about 65,000 messages and dealing with email can be half...the first couple of hires to build a strong, collaborative team. And take advantage of the many resources...Medical School) for taking the time to speak with us about adding new scientists to their labs. Further Reading...

  15. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ......perhaps you get the idea. What will you start? 4. Do informational interviews (on a schedule) I hope...mentoring group (see above). However, now that we are all so good with video calls, how about trying your hand...blog or podcast, initiated and maintained a collaborative project, served on the board of a professional...appointments with people all over the world. Think about contacting alumni of your lab or university or use...suggest you speak with. See this post that’s all about informational interviews, for more details on how...progress on both building relationships and thinking about future career options. 5. Write a grant - even if...

  16. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...Schmidt H, Krause MW, Seydoux G. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014...just one injection step! The entire protocol takes about 2-3 weeks but requires less than eight hours worth... this species, namely, the robust HDR mentioned above, an accessible germline, and multiple selectable...modular system that requires very little hands-on labor and could facilitate the tagging of every protein...

  17. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...same name. The tag has a net negative charge with 4 negatively charged and 1 positively charged amino ...Tags have a lot of major benefits – as discussed above – but there are a few instances when they should... hindrances, and destabilization can be brought about by the tag so it’s important to validate tagged ...

  18. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...Next-generation Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Maynard...share the procedures and Python scripts with laboratories and viral vector cores routinely producing AAV...on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols for working...

  20. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ...formation as well as increased Zfp462 expression within 4 hours of blue light induction (6-fold faster than ... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...neurons. Earlier this year, the Hegemann lab and collaborators report their discovery of a new family of seven...
Showing: 181 - 200 of 340 results